1. Immunoinformatics approach to design next-generation epitope-based peptide vaccine against Peste des Petits Ruminants Virus (PPRV).
- Author
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Riaz, Rimsha, Zahid, Saira, and Khan, Muhammad Sarwar
- Subjects
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PESTE des petits ruminants , *PEPTIDES , *T helper cells , *TOLL-like receptors , *KILLER cell receptors , *MOLECULAR docking , *BIOLOGICAL systems , *B cells - Abstract
PPR is a small ruminant infectious illness that is wreaking havoc on economies in South Asia and Africa. Due to several hurdles, such as resistant strains and a limited regulatory framework, existing therapeutic vaccinations have failed to eradicate the disease. To overcome these challenges, a peptide-based vaccination with distinct immunological characteristics and less vulnerability to contamination, autoimmunity, and inflammatory diseases would be useful in accomplishing the suggested eradication goal. The possible CTL and B-cell epitopes of PPRV's four immunogenic proteins (H, F, M, and N) were predicted using a variety of immunoinformatic methods. Protein-protein docking confirmed that the proposed chimeric vaccine has a considerable affinity for the ovine toll-like receptor. In a biological system, the molecular dynamic simulation confirmed the in vivo stability of the protein. The immune simulation predicted the induction of immunoglobulins, interleukins, interferons, helper T cells, and cytotoxic T cells, which is critical for boosting host immunity during infection. Moreover, the translation efficiency was verified through in silico cloning of the vaccine in a bacterial expression system following codon optimization. Thus, our findings suggest that the candidate vaccine will help in provoking a robust and long-lasting immunity in animals against PPRV infection. [Display omitted] • Genotype IV of the PPR virus is currently circulating in Pakistan. • Designing of a multi-epitope vaccine against Peste des Petits Ruminants Virus (PPRV). • Chimeric vaccine has a strong binding affinity with ovine Toll-Like Receptor 6. • Both, molecular dynamic and immune simulations validated the vaccine's effectiveness. [ABSTRACT FROM AUTHOR] more...
- Published
- 2023
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