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The article focuses on a study related to isolation of secondary metabolites from the sponge-associated fungus nigrospora oryzae. It informs that the fungus was isolated from the marine sponge Clathrina luteoculcitella, which is collected off Yongxing Island in the South China Sea. It also informs that dichlorobenzoic acid and diketopiperazines isolated from fungus exhibit diverse and remarkable biological activities, such as antimicrobial, antioxidant, and antitumor effect.