1. Complete genome sequence of a new achyranthes virus A isolate from Achyranthes bidentata in China.
- Author
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Wu, Xinyang, Lai, Yuchao, Lv, Lanqing, Han, Kelei, Chen, Ziqiang, Lu, Yuwen, Peng, Jiejun, Lin, Lin, Chen, Jianping, Zheng, Hongying, and Yan, Fei
- Subjects
REVERSE transcriptase polymerase chain reaction ,NUCLEOTIDE sequencing - Abstract
We have determined the complete genomic sequence of a potyvirus from Achyranthes bidentata in Zhejiang, China, using reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) PCR. The genomic RNA is 9482 nucleotides (nt) long excluding the 3′-terminal poly(A) tail and encodes a putative large polyprotein with 3073 amino acids (aa). It has 75.4–53.5% nt sequence identity and 84.0–49.1% polyprotein sequence identity to other potyviruses and is probably a distantly related isolate of the same species as the recently reported achyranthes virus A isolate from South Korea (AcVA-SK). This is the first report of the occurrence of a potyvirus infecting A. bidentata in China. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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