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Background/Aims Helicobacter pylori has been studied as an original bacteria to attack gastric mucosa and cause chronic infections such as gastritis, ulcer and cancer. The clinic has analyzed positive rate of H. pylori by CLOtest conducted with biopsy about abnormal lesion observed during transnasal endoscopy because it is important to understand H. pylori in Korea where is high rate of gastric cancer and H. pylori infection. Methods We had examined H. pylori positive or negative on CLOtest 1,627 cases conducted with biopsy of gastric lesion using transnasal endoscope from February 3, 2016 to July 31, 2019. The used equipments were EG-530N, 5.9 mm diameter, transnasal endoscopy and CLOtest kit. Results The 52.5% rate of positive was reported since 855 cases out of 1,627 cases on CLOtest were positive. Gastric cancer 0.67% and gastric atypia 5.16% were reported since 11 cases and 84 cases out of 1,627 cases. Conclusions The existence of H. pylori or not from the analysis of relation between biopsy result and H. pylori infection on CLOtest conducted with gastric lesion during gastric endoscopy will be an important factor for the follow up observation to find gastric cancer early and prevent cancer through H. pylori eradication. [ABSTRACT FROM AUTHOR]