1. Genes coding for Shiga-like toxins in bovine verotoxin-producing Escherichia coli (VTEC) strains belonging to different O:K:H serotypes.
- Author
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Blanco M, Blanco J, Blanco JE, González EA, Gomes TA, Zerbini LF, Yano T, and de Castro AF
- Subjects
- Animals, Cattle Diseases microbiology, DNA Probes, Diarrhea microbiology, Diarrhea veterinary, Disease Reservoirs, Enterotoxins genetics, Escherichia coli classification, Escherichia coli pathogenicity, Humans, Serotyping, Shiga Toxin 1, Shiga Toxin 2, Spain, Bacterial Toxins biosynthesis, Bacterial Toxins genetics, Cattle microbiology, Escherichia coli genetics, Genes, Bacterial
- Abstract
Forty-six verotoxin-producing Escherichia coli (VTEC) strains isolated from diarrhoeic and healthy calves in Spain were examined for DNA sequences homologous to genes for verotoxins (VT1 and VT2) and enterotoxins (LT-I, LT-II, STaH, STaP and STb). Hybridisation showed that 26 (57%) of VTEC strains carried VT1 genes, 13 (28%) possessed VT2 genes, and 7 (15%) carried both VT1 and VT2 genes. No VTEC strains hybridised with DNA probes for enterotoxins. A correlation was found between the serotype and type of VT produced. Thus, all strains of serotypes O26:K-:H11 (13 strains), O103:K-:H2 (3 strains) and O128:K?:H- (4 strains) hybridised with the VT1 probe only, whereas all strains of serotypes O4:K-:H4 (3 strains) and O113:K-:H21 (4 strains) were positive with the VT2 probe only. By contrast, O81:K?:H28 (2 strains) and O157:K-:H- (2 strains) strains hybridised with both VT1 and VT2 probes. One strain of serotype O157:K-:H7 was VT2 positive.
- Published
- 1994
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