1. Development of an enzyme-linked immunosorbent assay for octylphenol.
- Author
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Li, Zhuokun, Liu, Liqiang, Song, Shanshan, Guo, Shidong, Kuang, Hua, and Xu, Chuanlai
- Subjects
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ENZYME-linked immunosorbent assay , *NONYLPHENOL , *HAPTENS , *CARRIER proteins , *MONOCLONAL antibodies , *QUANTITATIVE research - Abstract
An indirect enzyme-linked immunosorbent assay (ELISA) was developed for the quantitative analysis of octylphenol. A linear carboxylated analogue of 4-nonylphenol was synthesised and characterised as hapten. The hapten was coupled to the carrier protein to produce a specific antibody. An indirect competitive ELISA method was established based on the polyclonal antibody, which exhibited an IC50value of 51 ng/mL for octylphenol. A monoclonal antibody was produced with an IC50value of 76 ng/mL for octylphenol. The immunoassay used with the monoclonal antibodies was applied to analyse water samples from Tai Lake in China. [ABSTRACT FROM PUBLISHER]
- Published
- 2014
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