1. Matrix metalloproteinase-3 promoter polymorphisms but not dupA-H. pylori correlate to duodenal ulcers in H. pylori-infected females.
- Author
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Yeh YC, Cheng HC, Chang WL, Yang HB, and Sheu BS
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Asian People genetics, Duodenal Ulcer enzymology, Duodenal Ulcer microbiology, Female, Genetic Predisposition to Disease, Genotype, Helicobacter Infections enzymology, Helicobacter Infections microbiology, Helicobacter pylori isolation & purification, Helicobacter pylori metabolism, Humans, Male, Matrix Metalloproteinase 3 metabolism, Matrix Metalloproteinases genetics, Matrix Metalloproteinases metabolism, Middle Aged, Sex Factors, Taiwan, Virulence Factors metabolism, Young Adult, Duodenal Ulcer genetics, Helicobacter Infections genetics, Helicobacter pylori genetics, Matrix Metalloproteinase 3 genetics, Polymorphism, Genetic, Virulence Factors genetics
- Abstract
Background: This study investigated if the H. pylori dupA genotype and certain host single nucleotide polymorphisms (SNPs) of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs), including MMP-3, MMP-7, MMP-9, TIMP-1 and TIMP-2, might correlate with ulcer risk of H. pylori-infected Taiwanese patients., Results: Of the 549 H. pylori-infected patients enrolled, 470 patients (265 with gastritis, 118 with duodenal ulcer, and 87 with gastric ulcer) received SNPs analysis of MMP-3-1612 6A > 5A, MMP-7-181 A > G, MMP-9exon 6 A > G, TIMP-1372 T > C and TIMP-2-418 G > C by PCR-RFLP. The 181 collected H. pylori isolates were detected for the dupA genotype by PCR. The rates of dupA-positive H. pylori infection were similar among patients with duodenal ulcer (22.8%), gastric ulcer (20.0%), and gastritis (25.5%) (p > 0.05). Males had higher rates of duodenal ulcer and gastric ulcer than females (p < 0.01). Of H. pylori-infected patients, the MMP-3 6A6A genotype were more common in patients with duodenal ulcers than in those with gastritis (87.7% vs. 74.9%, p < 0.05) in females. This genotype had a 2.4-fold (95% CI: 1.02-5.66) increased risk of duodenal ulcer, compared to those with the 5A carrier. Combining the MMP-3/TIMP-1 genotype as 6A6A/CC, the risk of duodenal ulcer increased up to 3.6 fold (p < 0.05) in H. pylori-infected females., Conclusions: The MMP-3 promoter polymorphism, but not the dupA-status, may correlate with susceptibility to duodenal ulcer after H. pylori infection in Taiwanese females.
- Published
- 2010
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