1. Optimal Production of Ganoderma formosanum Mycelium with Anti-Melanogenic Activity.
- Author
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Liu, Yen-Wenn, Liu, Chi-Mei, Chen, Hung-Yueh, Khumsupan, Darin, Hsu, Hsien-Yi, Lin, Hui-Wen, Hsieh, Chang-Wei, and Cheng, Kuan-Chen
- Subjects
MELANINS ,MYCELIUM ,GANODERMA ,ETHYL acetate ,PHENOL oxidase ,HYPOPIGMENTATION - Abstract
Ganoderma formosanum is a medicinal mushroom endemic to Taiwan. The extraction of G. formosanum mycelium using ethyl acetate showed a significant reduction of melanin activity due to the inhibition of tyrosinase. To optimize the production of G. formosanum mycelium with anti-melanogenic activity, different variables (carbon sources and concentrations, initial pH value, and temperature) were investigated. According to the results, the optimal conditions included the utilization of 50 g/L lactose as the carbon source at pH 7 and 25 °C for 9 days. Afterward, the G. formosanum ethanolic extracts-optimized (GFE-O) was used to study the anti-melanogenic activity in vitro and in vivo. In B16-F10 melanoma cells treated with GFE-O (0.1 mg/mL), the intracellular melanin content was reduced to 76% compared to the control group. By applying GFE-O (0.05 mg/mL) in vivo on zebrafish embryo, hypopigmentation was observed, and the melanin content was reduced to 62% compared to the control with no toxicological effects. The results showed that the optimal inoculation conditions can provide the basis for future large-scale production of G. formosanum mycelium to maximize the extraction of anti-melanogenic ingredients. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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