1. Identification of sulfoglycolipid bioactivities and characteristic fatty acids of marine macroalgae.
- Author
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Tsai CJ and Sun Pan B
- Subjects
- Antineoplastic Agents pharmacology, Aquatic Organisms, Chlorophyta chemistry, Eicosapentaenoic Acid analysis, Fatty Acids chemistry, Fatty Acids, Unsaturated analysis, Fatty Acids, Unsaturated classification, Hep G2 Cells drug effects, Humans, Inhibitory Concentration 50, Rhodophyta chemistry, Taiwan, Fatty Acids analysis, Glycolipids chemistry, Glycolipids pharmacology, Seaweed chemistry
- Abstract
The fatty acid compositions of 21 species of marine macroalgae, including 5 species of Chlorophyta (green algae), 13 of Rhodophyta (red algae), and 3 of Heterokontophyta (brown algae), were collected from northeastern Taiwan to survey their functional lipids. The lipid contents of green algae ranged from 15.36 to 20.15 mg/g, dry basis (db), and were characterized by a high content of C18:2 and C18:3, red algae (18.57-28.34 mg/g db) were high in C20:4 and C20:5, and brown algae (13.11-19.56 mg/g db) were high in C18:4, C20:4, and C:20:5. All algal lipids contained fatty acids of odd-number carbons, C17:0, and C17:1. Red algae had relatively higher levels of polyunsaturated fatty acids (PUFAs) and were richer in eicosapentaenoic acid (EPA) than green and brown algae. A red alga, Porphyra crispata , was extracted with ethanol and separated on a hydrophobic column (Diaion HP-20 column) to obtain sulfoglycolipids (sulfoquinovosyldiacylglycerols, SQDGs). The main fatty acids in SQDGs were palmitic acid (C16:0), 33.3%; EPA (C20:5), 30.0%; arachidonic acid (C20:4), 12.7%; oleic acid (C18:1), 7.52%; and stearic acid (C18:0), 6.83%. The n-3/n-6 ratio was 1.9, whereas the authentic standard, spinach SQDG, did not contain n-3 fatty acids. Sulfoglycolipids inhibited the growth of human hepatocellular carcinoma cell line (HepG2). The IC50 was 126 μg/mL, which is lower than that of the spinach SQDG (255 μg/mL).
- Published
- 2012
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