1. Proteomics-based identification of plasma biomarkers in oral squamous cell carcinoma.
- Author
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Tung CL, Lin ST, Chou HC, Chen YW, Lin HC, Tung CL, Huang KJ, Chen YJ, Lee YR, and Chan HL
- Subjects
- Adult, Areca toxicity, Biomarkers blood, Biomarkers chemistry, Carcinoma, Squamous Cell etiology, Disease Progression, Female, Head and Neck Neoplasms blood, Head and Neck Neoplasms etiology, Humans, Male, Middle Aged, Mouth Neoplasms etiology, Nuts toxicity, Peptide Mapping, Proteomics methods, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Squamous Cell Carcinoma of Head and Neck, Substance-Related Disorders physiopathology, Taiwan, Two-Dimensional Difference Gel Electrophoresis, Vitamin D-Binding Protein chemistry, Carcinoma, Squamous Cell blood, Down-Regulation, Mouth Neoplasms blood, Vitamin D-Binding Protein blood
- Abstract
Oral squamous cell carcinoma (OSCC) is an aggressive cancer and its occurrence is closely related to betel nut chewing in Taiwan. However, there are few prognostic and diagnostic biomarkers for this disease especially for its association with betel nut chewing. Recent progresses in quantitative proteomics have offered opportunities to discover plasma proteins as biomarkers for tracking the progression and for understanding the molecular mechanisms of OSCC. In present study, plasma samples from OSCC patients with at least 5-year history of betel nut chewing and healthy donors were analyzed by fluorescence 2D-DIGE-based proteomic analysis. Totally, 38 proteins have been firmly identified representing 13 unique gene products. These proteins mainly function in inflammatory responses (such as fibrinogen gamma chain) and transport (Apolipoprotein A-I). Additionally, the current quantitative proteomic approach has identified numerous OSCC biomarkers including fibrinogen (alpha/beta/gamma) chain, haptoglobin, leucine-rich alpha-2-glycoprotein and ribosomal protein S6 kinase alpha-3 (RSK2) which have not been reported and may be associated with the progression and development of the disease. In summary, this study reports a comprehensive patient-based proteomic approach for the identification of potential plasma biomarkers in OSCC. The potential of utilizing these markers for screening and treating OSCC warrants further investigations., (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Published
- 2013
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