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1. [Analysis of the organization of immunodominant epitopes of hepatitis virus E orf2 and orf3 proteins using synthetic peptides].

Author

Dement'eva EV, Semiletov IuA, Iashina TL, and Shibnev VA

Subjects
Antibody Specificity, Disease Outbreaks, Genome, Viral, Hepatitis E epidemiology, Hepatitis E genetics, Humans, Immunodominant Epitopes immunology, Immunoenzyme Techniques, Species Specificity, Tajikistan epidemiology, Uzbekistan epidemiology, Hepatitis E immunology, Hepatitis E virus genetics, Immunodominant Epitopes chemistry, Viral Proteins immunology
Abstract

A set of synthetic peptides of different length derived from proteins encoded by open reading frames (ORF) 1, 2, and 3 of hepatitis E virus (HEV) genome was synthesized and tested for antigenicity in indirect enzyme immunoassay. Peptides 414-433 (ORF2 protein) and 99-119 (ORF3) specifically react with antiHEV-positive sera collected during outbreaks in the Central Asian areas of the former USSR. The antigenic structure of the Central Asian variants and the Burmese types of HEV was found similar.

Published
1997

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