1. Isolation and characterization of Escherichia coli O157 from retail beef and bovine feces in Thailand.
- Author
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Vuddhakul V, Patararungrong N, Pungrasamee P, Jitsurong S, Morigaki T, Asai N, and Nishibuchi M
- Subjects
- Animals, Antibodies, Bacterial blood, Bacterial Toxins genetics, Cattle, Culture Media, DNA Fingerprinting, Escherichia coli Infections microbiology, Escherichia coli O157 classification, Escherichia coli O157 genetics, Escherichia coli O157 immunology, Food Handling, Humans, Immunomagnetic Separation, O Antigens immunology, Plasmids genetics, Shiga Toxins, Thailand, Escherichia coli O157 isolation & purification, Feces microbiology, Meat microbiology
- Abstract
Antibody to Escherichia coli O157 lipopolysaccharide was detected in the sera of healthy individuals more frequently in Southern Thailand than in Japan. The result suggested possible exposure of Thai people to E. coli O157. E. coli O157:H7 or O157:H(-) was isolated from four of 95 retail beef and one of 55 bovine feces samples collected in Southern Thailand by enrichment culture followed by immunomagnetic bead separation. Four of the five strains carried the stx(2) gene alone or in combination with the stx(1) gene. The strains were shown to be genetically distinct by an arbitrarily primed PCR method.
- Published
- 2000
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