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Presently, the prevalence of antibiotic resistance genes (ARGs) is regarded as an emerging environmental issue, and many studies have illuminated biogeographical patterns of the antibiotic resistome. However, few studies have investigated elevational biogeography and associated assembly mechanisms of ARGs in natural river systems. Accordingly, in the present study, we used metagenomics approaches to analyze the biogeographical pattern of ARGs along the pristine Yarlung Tsangpo River on the Tibetan Plateau. Our study retrieved the baseline profiles of ARGs in the pristine river and showed that the ARGs were dominated by bacA, which was resistant to bacitracin and represented more than 91% of total ARGs. The diversity and abundance of ARGs in the pristine river were lower than those in the human-impacted area, suggesting that the antibiotic resistome evolved and was promoted in a human-impacted environment. Furthermore, an elevational distance-decay relationship of ARGs was observed along the pristine Yarlung Tsangpo River, which provided strong evidence of ARG dissimilarity under the elevational variation. Elevational gradients could lead to changes in environmental variables and spatial factors, and consequently alter ARG composition. Elevational gradients could influence the assembly processes of ARGs. The deterministic and stochastic assembly processes both played critical roles and equally participated in shaping ARG composition at the watershed scale along the Yarlung Tsangpo River, and with increasing elevational variation along the river, the ecological processes of ARG assembly shifted from deterministic to stochastic. Moreover, abundant and rare ARGs were tentatively separated to investigate the difference and similarity in their distributions. Although abundant and rare ARGs presented similar distance-decay relationships, rare ARGs were more diverse and vulnerable to the dispersal process and mutation. Overall, our study provides valuable ecological insights to profile the large-scale elevational patterns of ARGs in a pristine river system, thereby providing important information for public health and environmental management., (Copyright © 2021. Published by Elsevier Ltd.)

Human activities can accelerate the antibiotic resistome prevalence and pose threats to ecological safety and public health globally. However, antibiotic resistance gene (ARG) mobility and dissemination into bacterial pathogens under anthropogenic disturbances are still poorly understood. Here, we used a metagenomic approach to profile the biogeography of ARGs and pathogenic antibiotic resistant bacteria (PARB) under anthropogenic disturbances along the Yarlung Tsangpo River. Results showed the ARGs was dominated by bacA gene along the Yarlung Tsangpo River on the Tibetan Plateau. The ARG composition was differently impacted by rapid urbanization and dam construction, which urbanization could promote ARGs resistant to sulfonamide and tetracycline, whereas dam construction could elevate the resistance to chloramphenicol and aminoglycoside. Land use pattern was identified as a critical factor influencing ARG composition under anthropogenic disturbances, as it could directly reflect the land degradation level and indicate the inputs of ARG-selective chemicals of different human activities. Moreover, despite of the lack of variation in ARG relative abundance, PARB were highly promoted by anthropogenic activities, indicating increasing ARG dissemination to pathogen. We found that human-impacted environments harbored high proportion of mobile genetic elements (MGEs), and the MGE carrying ARGs also increased under anthropogenic disturbances in the pathogenic hosts, which confirmed that anthropogenic activities could promote ARG horizontal gene transfer. Furthermore, anthropogenic activities could influence PARB assembly processes. Basically, stochastic processes dominated PARB assembly along the river, and with increasing level of anthropogenic activities, these processes shifted from undominated stochastic processes to dispersal limitation. In summary, this study provides useful strategies in watershed resistome management and reduction of ARG dissemination to pathogens, which should consider the mode and intensity of human activity and its potential influence on horizontal gene transfer and assembly processes., (Copyright © 2021. Published by Elsevier Ltd.)

River systems have critical roles in the natural water environment and the transportation of nutrients. Anthropogenic activities, including wastewater discharge and river damming, raise adverse impacts on ecosystem and continuum of rivers. An increasing amount of attention has been paid to riverine bacterioplankton as they make vital contributions to biogeochemical nutrient cycle. A comprehensive study was conducted on the bacterioplankton community along the Yarlung Tsangpo River, which is the longest plateau river in China and is suffering from various anthropogenic impacts. The results indicated that nutrient variations corresponded to anthropogenic activities, and silica, nitrogen and phosphorus were retained by the dam. River damming influenced the biomass and diversity of the bacterioplankton, but significant alterations in the community structure were not observed between upstream and downstream of the dam. Moreover, the spatial distribution of the bacterioplankton community changed gradually along the river, and the dominant bacterioplankton in the upstream, midstream and downstream portions of the river were Firmicutes, Bacteroidetes and Proteobacteria, respectively. Soluble reactive phosphorus, elevation, ammonium nitrogen, velocity and turbidity were the main environmental factors that shape the bacterioplankton community. Our study offers the first insights into the variation of a bacterioplankton community of a large river in plateau region.