1. Genetic diversity and classification of Tibetan yak populations based on the mtDNA COIII gene.
- Author
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Song QQ, Chai ZX, Xin JW, Zhao SJ, Ji QM, Zhang CF, Ma ZJ, and Zhong JC
- Subjects
- Animals, Cattle classification, DNA, Mitochondrial genetics, Evolution, Molecular, Haplotypes, Phylogeny, Phylogeography, Polymorphism, Single Nucleotide, Sequence Analysis, DNA, Tibet, Cattle genetics, Electron Transport Complex IV genetics, Genetic Variation, Genetics, Population
- Abstract
To determine the level of genetic diversity and phylogenetic relationships among Tibetan yak populations, the mitochondrial DNA cytochrome c oxidase subunit 3 (COIII) genes of 378 yak individuals from 16 populations were analyzed in this study. The results showed that the length of cytochrome c oxidase subunit 3 gene sequences was 781 bp, with nucleotide frequencies of 29.2, 29.4, 26.1, and 15.2% for T, C, A, and G, respectively. A total of 26 haplotypes were identified, with 69 polymorphic sites, including 11 parsimony-informative sites and 58 single-nucleotide polymorphism sites. No deletions/insertions were found in sequence comparison, indicating that nucleotide mutation types were transitions and transversions. Haplotype and nucleotide diversities were 0.562 and 0.00138, respectively, indicating a high level of genetic diversity in Tibetan yak populations. Phylogenetic relationship analysis indicated that Tibetan yak populations are divided into 2 groups.
- Published
- 2015
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