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As a crucial component of the ecological security pattern, ecological source (ES) plays a vital role in providing ecosystem service value (ESV) and conserving biodiversity. Previous studies have mostly considered ES only from either landscape change pattern or ecological function perspectives, and have ignored their integration and spatio-temporal evolutionary modeling. In this study, we proposed a multi-perspective framework for the spatio-temporal characteristics of ES by ESV incorporating landscape aesthetics, carbon sink characteristics, ecological quality, and kernel NDVI (kNDVI). By integrating the revised ESV and the kernel normalized difference vegetation index as a foundation, we employed the spatial priority model to identify ES. This improvement aims to yield a more practical and specific ESV result. Applying this framework to the Three-River Headwaters Region (TRHR), a significant spatio-temporal change in ecological sources has been observed from 2000 to 2020. This performance provided a reference for ecological conservation in the TRHR. The results indicate that this ecological source identification framework has reliable accuracy and efficiency compared with the existing NRs in the TRHR. This method could reveal more precise spatio-temporal distributions of ES, enhancing ecosystem integrity and providing technical modeling support for developing cross-scale spatial planning and management strategies for nature reserve boundaries. The framework proposed in our research could serve as a reference for building ecological networks in other ecologically fragile areas. [ABSTRACT FROM AUTHOR]