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We investigated the contents inside the esophagus and stomach of turtles inside and outside of the Tokyo Bay area, which face high and low risks of microplastic (MP) exposure, respectively. 65 synthetic particles were recovered from 8 out of 22 turtles, using ATR-FTIR followed by density separation with calcium chloride solution. Statistical analysis indicated that turtles in high-risk areas ingested significantly more MPs than those in low-risk areas. As the inflow of MPs from major rivers influences pollution levels in the ocean, the results of this study highlight the importance of major rivers for MP ingestion by turtles. Additionally, we discussed the current methodology's shortcomings and addressed scope for subsequent research, along with suggestions on future conservation., Competing Interests: Declaration of competing interest The authors declare that they have no competing financial interests or personal relationships that may have influenced the work reported in this study., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Nitrogen dioxide (NO 2 ) is an air pollutant discharged from combustion of human activities. Nitrous acid (HONO), measured as NO 2 , is thought to impact respiratory function more than NO 2 . HONO and NO 2 have an equilibrium relationship, and their reaction is affected by climate conditions. This study was conducted to discuss the extent of HONO contained in NO 2 , depending on the level of urbanization. Whether climate conditions that promote HONO production enhanced the level of NO 2 measured was investigated using time series analysis. Climate and outdoor air pollution data measured in April 2009-March 2017 in urban (Tokyo, Osaka, and Aichi) and rural (Yamanashi) areas in Japan were used for the analysis. Air temperature had a trend of negative associations with NO 2 , which might indicate the decomposition of HONO in the equilibrium between HONO and NO 2 . The associations of relative humidity with NO 2 did not have consistent trends by prefecture: humidity only in Yamanashi was positively associated with NO 2 . In high relative humidity conditions, the equilibrium goes towards HONO production, which was observed in Yamanashi, suggesting the proportion of HONO in NO 2 might be low/high in urban/rural areas.