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Methods to improve the efficiency of bioremediation of hydrocarbon-contaminated sites involves the assessment of microbial consortia in culture. In the present study, the cooperative capabilities of indigenous bacteria, yeast and fungi, as naturally-occurring consortia isolated from chronically contaminated sites in Trinidad, were screened to determine the most efficient associations that resulted in maximum oil clearance. Thirty naturally-occurring consortia involving both known biosurfactant-producing and non-producing isolates were screened. A total of sixteen combinations of yeast with fungi and bacteria with fungi were found to be the most efficient at crude oil deterioration based on >70% a zone of clearance around the original inoculum site on different media. The study revealed a difference in degradation action of strain-specific combinations which confirmed that the development of microbial consortia is both strain- and site-specific. Those consortia composed of biosurfactant-producing members were the most efficient at crude oil removal. [ABSTRACT FROM AUTHOR]

Background and Aim: Access to specialized cardiac surgery is a problem in emerging countries. Here, we reflect on the approach we used to establish a cardiac surgery unit in Trinidad and Tobago. Methods: The program started in 1993 with monthly visits by a team from Bristol Heart Institute. A group of local doctors, nurses, and perfusionists were identified for training, and a senior nurse moved to the island to start a teaching program. The visiting support was gradually reduced, and the local team gained independence in managing the service in 2006. Results: The initial low volume surgery increased to around 380 cases a year with the implementation of comprehensive service in 2006. Most patients required coronary artery bypass graft (CABG). In‐hospital mortality declined from 5% in the nascent years to below 2% thereafter. In the last 5 years (2015‐2019), 1764 patients underwent surgery (mean age 59.6 ± 10.8 years, 66% male). The majority were East‐Indian‐Caribbean (79.1%) or Afro‐Caribbean (16.7%), half had diabetes, and two‐thirds hypertension (EuroScore II 1.8 ± 1.9). The majority (1363 patients) underwent CABG (99.5% off‐pump; conversion to on‐pump 1.5%). The mean number of grafts was 2.5 ± 0.7 with 98.5% and 23.1% receiving one and two or more arterial grafts, respectively. In‐hospital mortality was 1.1%, re‐exploration for bleeding 2%, stroke 0.1%, mediastinitis 0.2%. The length of the postoperative hospital stay was 5.8 ± 2 days. Conclusion: Frequent outside visits complemented by training in an overseas center, and transfer of knowledge proved to be an effective strategy to develop a cardiac surgery unit in an emerging country with results comparable to accepted international standards. [ABSTRACT FROM AUTHOR]

Background: Mannosylerythritol lipids (MELs) belong to the class of glycolipid biosurfactants and are produced by members of the Ustilago and Moesziomyces genera. Production of MELs is regulated by a biosynthetic gene cluster (MEL BGC). Extracellular lipase activity is also associated with MEL production. Most microbial glycolipid-producers are isolated from oil-contaminated environments. MEL-producing yeast that are capable of metabolizing crude oil are understudied, and there is very limited data on indigenous strains from tropical climates. Analysis of the MEL BGC and lipase genes in Trinidad M. antarcticus strains, using a gene-targeted approach, revealed a correlation between their intrinsic capability to degrade crude oil and their adaptation to survive in a chronically polluted terrestrial environment. Results: M. antarcticus was isolated from naturally-occurring crude oil seeps and an asphaltic mud volcano in Trinidad; these are habitats that have not been previously reported for this species. Genus identification was confirmed by the large-subunit (LSU) and the small-subunit (SSU) sequence comparisons and species identification was confirmed by ITS sequence comparisons and phylogenetic inference. The essential genes (Emt1, Mac1, Mac2, Mmf1) of the MEL BGC were detected with gene-specific primers. Emt1p, Mac1p and Mmf1p sequence analyses confirmed that the Trinidad strains harboured novel synonymous amino acid (aa) substitutions and structural comparisons revealed different regions of disorder, specifically for the Emt1p sequence. Functionality of each protein sequence was confirmed through motif mining and mutation prediction. Phylogenetic relatedness was inferred for Emt1p, Mac1p and Mmf1p sequences. The Trinidad strains clustered with other M. antarcticus sequences, however, the representative Trinidad M. antarcticus sequences consistently formed a separate, highly supported branch for each protein. Similar phylogenetic placement was indicated for LipA and LipB nucleotide and protein sequences. The Trinidad strains also demonstrated lipolytic activity in culture, with an ability to utilize different carbon sources. Comparative evolution of MEL BGC and LipA gene suggested early and late duplication events, depending on the gene, followed by a number of speciation events within Ustilaginaceae. M. antarcticus and M. aphidis were separated from all other members of Ustilaginaceae and two gene homologues were detected, one for each species. Conclusions: Sequence analyses was based on a novel gene-targeted approach to analyze the essential genes of the MEL BGC and LipA and LipB genes of M. antarcticus strains from Trinidad. The findings indicated that these strains accumulated nucleotide mutations to a threshold level that did not affect the function of specific proteins encoded by the MEL BGC and LipA and LipB genes. The biosurfactant and lipase enzymes secreted by these Trinidad M. antarcticus strains facilitated their survival in oil-contaminated terrestrial environments. These findings suggest that the Trinidad strains should be explored as promising candidates for the commercial production of MEL biosurfactants and lipase enzymes. [ABSTRACT FROM AUTHOR]

Asthma, a major cause of disability and reduced quality of life, has a high global prevalence and burden of death. Despite the propitious guidelines, a substantial portion of asthmatics reportedly have poorly controlled disease. In the current study, we have examined risk factors for uncontrolled asthma in specialty clinics and its association with impaired quality of life. A multicentre cross-sectional survey of asthma patients, 18 years and older, was conducted in Trinidad. Asthma Control Test (ACT) and the Juniper Mini Asthma Quality of Life Questionnaire (Mini AQLQ–J) were used to assess the disease control and quality of life, respectively. Data were analyzed using the Chi-square test and multivariable logistic regression controlling for gender. Of a total of 428 patients included, asthma was uncontrolled in 72.4% and asthma related quality of life was moderate to severely impaired in 86% of the studied population. In the multivariate regression models, poorly controlled asthma was associated with obesity (OR 2.25; 95% CI 1.30–3.39), late-onset asthma (OR 1.72; 95% CI 1.04–2.84), features of sleep apnea (OR 1.77; 95% CI 1.01–3.07) and depression (OR 2.01; 95% CI 1.04–3.86). Impaired quality of life was associated with Indo-Caribbean ethnicity (OR 3.19; 95% CI = 1.68–6.06). In this Caribbean population, uncontrolled asthma was independently associated with obesity, late-onset disease, and comorbidities of sleep apnea and depression. Poor asthma-related quality of life was independently associated with Indo-Caribbean ethnicity. [ABSTRACT FROM AUTHOR]

Mayaro virus, which can often go undetected due to its clinical manifestations and intimate alignment with dengue and chikungunya viruses, is one of the most neglected arboviruses. The virus has been found in several outbreaks, where a moderate-to-severe and potentially incapacitating joint disease has been observed. MAYV usually circulates in a sylvan cycle of forest mosquitoes and vertebrates, causing sporadic sylvatic infections to humans, and some outbreaks in sub-urban areas. This study focuses on the demonstration of the possible co-circulation of Mayaro virus with chikungunya virus and Zika virus during the outbreaks that occurred in Trinidad and Tobago in 2014 and 2016, respectively. Acute samples from patients who previously tested negative for chikungunya, dengue, and Zika, and specifically exhibiting joint pain were selected and investigated for the presence of Mayaro virus genome using real-time RT-PCR techniques. Nine persons were shown to be positive for Mayaro virus during the chikungunya outbreak of 2014, while no one during the Zika outbreak in 2016. Five results correspond to persons living in highly urbanized areas across Trinidad. These findings provide evidence that multiple arboviral circulations are possible and could easily go undetected especially during outbreak situations. Our study is the first to demonstrate the possible co-circulation of Mayaro and chikungunya viruses and the occurrence of human cases for both diseases during an outbreak in the Caribbean. A possible change in the pattern of distribution of human cases to more urbanized areas is also discussed. [ABSTRACT FROM AUTHOR]

This article examines the long-run relationship among energy exports, manufacturing exports, and gross domestic product (GDP) per capita in Trinidad and Tobago using annual data from 1971 to 2015. Using the autoregressive distributed lag bounds test approach to determine cointegration and long-run estimates among the variables and the Toda-Yamamoto test to examine longrun causality between the variables, we find a positive relationship between energy exports and GDP per capita and a negative relationship between manufacturing exports and GDP per capita. We also find evidence of both unidirectional causality and bidirectional causality among the variables, a finding that has implications for policy recommendations. [ABSTRACT FROM AUTHOR]

Background and Aim: The coronavirus disease 2019 (COVID‐19) pandemic has seen the cancellation of elective cardiac surgeries worldwide. Here we report the experience of a cardiac surgery unit in a developing country in response to the COVID‐19 crisis. Methods: From 6th April to 12th June 2020, 58 patients underwent urgent or emergency cardiac surgery. Data was reviewed from a prospectively entered unit‐maintained cardiac surgery database. To ensure safe delivery of care to patients, a series of strict measures were implemented which included: a parallel healthcare system maintaining a COVID‐19 cold site, social isolation of patients for one to 2 weeks before surgery, polymerase chain reaction testing for COVID‐19, 72 hours before surgery, discrete staff assigned only to cardiac surgical cases socially isolated for 2 weeks as necessary. Results: The mean age at surgery was 59.7 ± 11 years and 41 (70.7%) were male. Fifty‐two patients were hypertensive (90%), and 32 were diabetic (55.2%). There were three emergency type A aortic dissections. Forty‐seven patients underwent coronary artery bypass graft surgery with all but three performed off‐pump. Fourteen cases required blood product transfusion. One patient had postoperative pneumonia associated with chronic obstructive pulmonary disease. The median length of stay was 5.7 ± 1.8 days. All patients were discharged home after rehabilitation. There were no cases of COVID‐19 infection among healthcare workers during the study period. Conclusion: These strategies allowed us to maintain a service for urgent and emergency procedures and may prove useful for larger countries when there is decrease in COVID‐19 cases and planning for the restart of elective cardiac surgery. [ABSTRACT FROM AUTHOR]

Colletotrichum species complexes are among the top 10 economically important fungal plant pathogens worldwide because they can infect climacteric and nonclimacteric fruit at the pre and/or postharvest stages. C. truncatum is the major pathogen responsible for anthracnose of green and red bell pepper fruit worldwide. C. brevisporum was recently reported to be a minor pathogen of red bell pepper fruit in Trinidad, but has recently been reported as pathogenic to other host species in other countries. The ability of these phytopathogens to produce and secrete cutinase is required for dismantling the cuticle of the host plant and, therefore, crucial to the necrotrophic phase of their infection strategy. In vitro bioassays using different lipid substrates confirmed the ability of C. truncatum and C. brevisporum isolates from green and red bell peppers to secrete cutinase. The diversity, structure and organization and synteny of the cutinase gene were determined among different Colletotrichum species. Cluster analysis indicated a low level of nucleotide variation among C. truncatum sequences. Nucleotide sequences of C. brevisporum were more related to C. truncatum cutinase nucleotide sequences than to C. gloeosporioides. Cluster patterns coincided with haplotype and there was evidence of significant positive selection with no recombination signatures. The structure of the cutinase gene included two exons with one intervening intron and, therefore, one splice variant. Although amino acid sequences were highly conserved among C. truncatum isolates, diversity "hot spots" were revealed when the 66‐amino acid coding region of 200 fungal species was compared. Twenty cutinase orthologues were detected among different fungal species, whose common ancestor is Pezizomycotina and it is purported that these orthologues arose through a single gene duplication event prior to speciation. The cutinase domain was retained both in structure and arrangement among 34 different Colletotrichum species. The order of aligned genomic blocks between species and the arrangement of flanking protein domains were also conserved and shared for those domains immediately located at the N‐ and C‐terminus of the cutinase domain. Among these were an RNA recognition motif, translation elongation factor, signal peptide, pentatricopeptide repeat, and Hsp70 family of chaperone proteins, all of which support the expression of the cutinase gene. The findings of this study are important to understanding the evolution of the cutinase gene in C. truncatum as a key component of the biotrophic–necrotrophic switch which may be useful in developing gene‐targeting strategies to decrease the pathogenic potential of Colletotrichum species. [ABSTRACT FROM AUTHOR]

The aim of this research was to find the extent to which the literacy levels in reading comprehension of students entering a secondary school with low levels of reading and writing, would improve through content enhancement to aid in their cognitive development. The research was conducted in a secondary school in an educational district in Trinidad and Tobago that has one of the lowest primary school performance in the country. Form 1 Geography students at the secondary school, aged 11-13, expressed frustration in understanding geographical terms and concepts, thereby preventing them becoming proficient in the subject. They felt that teachers should help them understand the complex jargon in the subject. The Qualitative Reading Inventory-4 (QRI-4) diagnostic test was conducted prior to the intervention, to assess literacy levels for grade levels 4-6. Results from the diagnostic test indicated that the students' literacy level was below grade 7, which is the required level for Form 1. An action research approach, using three literacy strategies, namely, the Frayer Model, Audience-Centred teaching and Learning Logs, were implemented as the intervention during and at the end of each lesson over five weeks. Numerical and nonnumerical data on students' performance and attitude to reading were collected and analysed. Overall, students improved in their reading comprehension, which resulted in an elevated level of writing through Audience-Centred Teaching, advancement in vocabulary use with the Frayer model, and student self-reflection by using Learning Logs. They made connections between text in the lesson, other subjects and their real life. They were also more engaged in class as they began to read, write and construct meaning in Geography. The main implication of this study is that all teachers should consider integrating literacy strategies within their classroom practice to assist their students in becoming proficient in reading comprehension. [ABSTRACT FROM AUTHOR]

Purpose: A situational analysis of breast cancer (BC) early detection services was carried out to investigate whether Trinidad and Tobago (T&T) has the framework for successful organized national screening.Methods: An online survey was designed to assess the availability, accessibility, quality control and assurance (QC&A), and monitoring and evaluation (M&E) mechanisms for public and private BC early detection. A focus group with local radiologists (n = 3) was held to identify unaddressed challenges and make recommendations for improvement.Results: Major public hospitals offer free detection services with wait times of 1-6 months for an appointment. Private institutions offer mammograms for TTD$240 (USD$37) at minimum with same day service. Both sectors report a lack of trained staff. Using 1.2 mammograms per 10,000 women ≥40 years as sufficient, the public sector's rate of 0.19 mammograms per 10,000 women ≥40 years for screening and diagnosis is inadequate. Program M&E mechanisms, QC&A guidelines for machinery use, delays in receipt of pathology reports, and unreliable drug access are further unaddressed challenges.Conclusion: T&T must first strengthen its human and physical resources, implement M&E and QC&A measures, strengthen cancer care, and address other impediments to BC early detection before investing in nationally organized BC screening. [ABSTRACT FROM AUTHOR]

SUMMARY Objectives To assess the attitude towards voluntary non-remunerated blood donation among blood donors in Trinidad and Tobago ( TRT). Background Blood donors in TRT are either family replacement (F/R, 87%) or remunerated (13%). There is chronic blood shortage and high seroreactivity for transfusion-transmissible infections ( TTI) in donors. Converting existing to voluntary non-remunerated donors ( VNRD) reduces the need to recruit news donors in achieving 100% VNRD. Methods A questionnaire-based, cross-sectional survey was conducted at two blood collection centres at an interval of 8 years. Donors were surveyed for sociodemographic characteristics, awareness of the blood shortage, previous donation behaviour, donor-beneficiary linkage if F/R, willingness to become VNRD and choice of motivators for converting to VNRD. Results A total of 400 and 595 donors respectively participated in Surveys 1 and 2, of whom 92·8 and 86·3% were F/R ( P < 0·001), respectively. In both surveys, 52% of participants were unaware of an existing blood shortage ( P = 0·983). Only 9·8 and 9·1% of participants expressed unwillingness to become VNRD ( P = 0·720). The main motivators to convert to VNRD were reminders from the centre (84%) and extended opening hours (78%) in Survey 1 as compared to confidence that donated blood was used properly (73%) and shortened waiting times to donate (73%) in Survey 2. Conclusion Despite low awareness of blood shortage, willingness to become VNRD was high among existing donors. Accountability and donor convenience underpinned the main motivators for converting to VNRD. [ABSTRACT FROM AUTHOR]

Bourdieu describes capital as the political building blocks of social order that give meaning to social accumulation and consumption. Through a combination of Bourdieu’s sociology and critical race theory, this sojourn into Afro-Trinidadian boys’ achievement seeks to elucidate an approach to understanding capital as inherently raced. This is premised on an interrogation of Trinidadian society as pigmentocratically structured, where lightness is rewarded with a myriad of social advantages, and darkness denigrated as illegitimate and ‘other’. The premium placed on lighter skin is manifested interdependently in the forms of social, economic and cultural capital. As such, the operation of capital as politic not only reflects societal structures of power and domination, but importantly also contributes in the maintenance of said structures. The concept of ‘Racialised facilitative capital’ follows as inseparably both facilitator of social status and as racialised process. [ABSTRACT FROM PUBLISHER]

Colletotrichum gloeosporioides sensu lato is widely distributed throughout temperate and tropical regions and causes anthracnose disease in numerous plant species. Development of effective disease management strategies is dependent on, among other factors, an understanding of pathogen genetic diversity and population stratification at the intraspecific level. For 132 isolates of C. gloeosporioides sensu lato collected from papaya in Trinidad, inter-simple-sequence repeat-polymerase chain reaction (ISSR-PCR) generated 121 polymorphic loci from five ISSR primers selected from an initial screen of 22 ISSR primers. The mean percentage of polymorphic loci was 99.18%. Bayesian cluster analysis inferred three genetic subpopulations, where group 1 consisted exclusively of isolates collected in the southern part of Trinidad whereas groups 2 and 3, although genetically distinct, were mixtures of isolates collected from both the northern and southern parts of Trinidad. Principal coordinates analysis and unweighted pair-group method with arithmetic mean phylogeny were concordant with Bayesian cluster analysis and supported subdivision into the three subpopulations. Overall, the total mean gene diversity was 0.279, the mean within-population gene diversity was 0.2161, and genetic differentiation for the Trinidad population was 0.225. Regionally, northern isolates had a lower gene diversity compared with southern isolates. Nei's gene diversity was highest for group I (h = 0.231), followed by group 2 (h = 0.215) and group 3 (h = 0.202). Genotypic diversity was at or near maximum for all three subpopulations after clone correction. Pairwise estimates of differentiation indicated high and significant genetic differentiation among the inferred subpopulations (Weir's 0 of 0.212 to 0.325). Pairwise comparisons among subpopulations suggested restricted gene flow between groups 1 and 2 and groups 1 and 3 but not between groups 2 and 3. The null hypothesis of random mating was rejected for all three inferred subpopulations. These results suggest that pathogen biology and epidemiology as well as certain evolutionary factors may play an important role in population substructuring of C. gloeosporioides sensu lato isolates infecting papaya in Trinidad. [ABSTRACT FROM AUTHOR]

The article focuses on people-centered governance in Trinidad and Tobago. On May 24, 2010, the People's Partnership party, which was led by Kamla Persad-Bissessar, won against the incumbent People's National Movement. The victory was considered historic since it was the first time a female prime minister won after the island gained independence in 1962. A number of safe and ethno-based seats for each major party resulted from the election. Two factors are associated with the notion of safe seats namely the ethnic pattern of voting and race-based However, third parties, coalitions, redrawing of the boundaries, house-padding, leadership and candidates may threaten safe-seat strongholds.

Objectives. The purpose of this study was to investigate the presence and serovar identity of Salmonella, at the national level, in farmed Muscovy ducks (Cairina moschata) in Trinidad and Tobago, and to compare the relative benefits of bacterial culture to those of polymerase chain reaction (PCR) for use in the routine detection and surveillance of Salmonella in these ducks. Methods. From March--September 2003, 110 fecal samples were collected from 82 farms across the islands of Trinidad and Tobago. Salmonella was isolated from fresh and frozen samples and the serotype of each was determined through bacterial culture. An in-house, nested PCR that detects all pathogenic Salmonella species was utilized in analyzing the samples. Results. Five samples were positive for Salmonella by bacterial culture, whereas 44 were positive by the nested PCR. Serovars isolated were Kiambu, Orion, Uganda, and two isolates from Group E1 whose H antigens could not be fully characterized. Of the samples, 87 (79%) gave equivalent PCR results for both enrichment broths--28 were positive for both and 59 were negative for both). However, 16 samples were positive for one broth, but not for the other, with the majority (14 of the 16) resulting positive for Selenite broth. PCR results for seven samples were inconclusive due to ambiguous band size or multiple bands near the expected band size. Conclusions. In Trinidad and Tobago, the Muscovy duck does not appear to be a significant source of S. typhimurium or S. enteritidis, but it does harbor other Salmonella species. In-house, nested PCR represents a simple, relatively inexpensive and potentially more sensitive method than bacterial culture for the routine surveillance of pathogenic Salmonella in the Muscovy duck. [ABSTRACT FROM AUTHOR]

The author presents reprints of series of articles discussing the reaction toward writer V. S. Naipaul winning the Nobel Prize in Literature in 2001 in newspapers of his native Trinidad and Tobago and from other scholars and other Nobel laureates. It includes appreciation and criticisms for his works discussed including "A House for Mr Biswas" and "The Enigma of Arrival".

The practice of psychiatry in Trinidad and Tobago has been influenced by the country's history.The influx of many colonizing forces and cultures over the past 500 years have resulted in interesting culturally based treatment modalities. Psychiatry today, remains a unique blend of current scientific knowledge purporting a neurobiological basis of diseases and ThirdWorld traditional practices based on superstition, religion and folk medicine. Under British rule, the march ofWestern psychiatry closely paralleled the management and the treatment of the insane in England.After 50 years of British colonization, the first ordinance was passed in 1849 for the custody at the Royal Gaol of insane persons charged with offences. In 1858, the Belmont Psychiatric Asylum was opened for the accommodation of 80 persons and in 1900 the St Ann's Asylum, that currently houses 900 patients.The first half of the twentieth century was a period of aggressive physical methods of treatment utilizing bromide mixtures, hyperthermia for syphyllis, insulin coma therapy, electronarcosis and transorbital leucotomy.This was discontinued following the introduction of psychotropic drugs in 1954. Following independence from Britain in 1962, a number of plans for the development of psychiatric services were submitted to the Government, but few were implemented. However the, following plans have been implemented: the creation of Psychiatric Units at the two General hospitals, a Sectorization Plan, a Mental Health Act and the recent creation of Regional Health Authorities with a new Mental Health Plan. Research over the past four decades have focused on social psychiatry, particularly alcoholism, attempted suicide and suicide. Over the last decade, research in psychiatry has grown from a trickle to a flood with renewed interest in epidemiology, schizophrenia, depression and biological psychiatry. Notwithstanding some difficulties, psychiatry today remains well organized with greater emphasis on institutional care than on community care.The revised Mental Health Plan now under consideration has shifted the focus to primary integrated care calling for an active intersectoral collaboration and involvement of individuals, families and communities. It is hoped that the new approach will incorporate both indigenous cures of ancient methods of treatment and modern scientific knowledge.The new millennium promises to be an exciting one for the practice of psychiatry in Trinidad and Tobago. [ABSTRACT FROM AUTHOR]

Trinidad and Tobago is the largest producer of oil and natural gas in Central America and the Caribbean. Natural crude oil seeps, in addition to leaking petroleum pipelines, have resulted in chronic contamination of the surrounding terrestrial environments since the time of petroleum discovery, production, and refinement in Trinidad. In this study, we isolated microbes from soils chronically contaminated with crude oil using a culture-dependent approach with enrichment. The sampling of eight such sites located in the southern peninsula of Trinidad revealed a diverse microbial composition and novel oil-degrading filamentous fungi and yeast as single-isolate degraders and naturally occurring consortia, with specific bacterial species not previously reported in the literature. Multiple sequence comparisons and phylogenetic analyses confirmed the identity of the top degraders. The filamentous fungal community based on culturable species was dominated by Ascomycota, and the recovered yeast isolates were affiliated with Basidiomycota (65.23%) and Ascomycota (34.78%) phyla. Enhanced biodegradation of petroleum hydrocarbons is maintained by biocatalysts such as lipases. Five out of seven species demonstrated extracellular lipase activity in vitro. Our findings could provide new insights into microbial resources from chronically contaminated terrestrial environments, and this information will be beneficial to the bioremediation of petroleum contamination and other industrial applications. [ABSTRACT FROM AUTHOR]

The authors offer their views whether or not Trinidad & Tobago should introduce Highly Active Antiretroviral Therapy (HAART) in the primary health care system. They comment on the use of HAART for treating HIV infections. They discuss the lack of continuity of care by primary care physicians on HIV patients.

The Fusarium incarnatum-equiseti species complex (FIESC) consists of 33 phylogenetic species according to multi-locus sequence typing (MLST) and Genealogical Concordance Phylogenetic Species Recognition (GCPSR). A multi-locus dataset consisting of nucleotide sequences of the translation elongation factor (EF-1α), calmodulin (CAM), partial RNA polymerase largest subunit (RPB1), and partial RNA polymerase second largest subunit (RPB2), was generated to distinguish among phylogenetic species within the FIESC isolates infecting bell pepper in Trinidad. Three phylogenetic species belonged to the Incarnatum clade (FIESC-15, FIESC-16, and FIESC-26), and one species belonged to the Equiseti clade (FIESC-14). Specific MLST types were sensitive to 10 µg/mL of tebuconazole fungicide as a discriminatory dose. The EC50 values were significantly different among the four MLST groups, which were separated into two homogeneous groups: FIESC-26a and FIESC-14a, demonstrating the "sensitive" azole phenotype and FIESC-15a and FIESC-16a as the "less sensitive" azole phenotype. CYP51C sequences of the Trinidad isolates, although under positive selection, were without any signatures of recombination, were highly conserved, and were not correlated with these azole phenotypes. CYP51C sequences were unable to resolve the FIESC isolates as phylogenetic inference indicated polytomic branching for these sequences. This data is important to different research communities, including those studying Fusarium phytopathology, mycotoxins, and public health impacts. [ABSTRACT FROM AUTHOR]

Purpose. There are many known variations in the arterial supply to the liver. We sought to document the incidence and details of anomalies of the extrahepatic arteries in an unselected population in the West Indies. Methods. This study spanned 24 months. All 205 CT scans were evaluated at a hepatobiliary referral center in Trinidad and Tobago. We described the anomalies of the arterial supply to the liver using the conventional classification proposed by Michels. Results. 205 CT scans were evaluated, and 112 persons (54.6%) had conventional Type 1 anatomy. However, compared to the incidence in the existing medical literature, we encountered a greater incidence of replaced right hepatic arteries (18.1% vs 11%; P 0.04) and a lower incidence of accessory right hepatic arteries (2.4% vs 7%; P 0.030). Conclusion. Although 54.6% of persons in this West Indian population have conventional hepatic arterial supply, the distribution of anatomic variants of the right hepatic artery is quite different to that seen in North American and European centers. We found a higher incidence of replaced right hepatic arteries and a lower incidence of accessory right hepatic arteries. [ABSTRACT FROM AUTHOR]