1. Evaluation of Antioxidant, Anti-Inflammatory and Antityrosinase Potential of Extracts from Different Aerial Parts of Rhanterium suaveolens from Tunisia.
- Author
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Chelly S, Chelly M, Occhiuto C, Cimino F, Cristani M, Saija A, Molonia MS, Ruberto G, D'Angelo V, Germanò MP, Siracusa L, Bouaziz-Ketata H, and Speciale A
- Subjects
- Animals, Anti-Inflammatory Agents isolation & purification, Anti-Inflammatory Agents metabolism, Anti-Inflammatory Agents pharmacology, Antioxidants chemistry, Asteraceae metabolism, Caco-2 Cells, Cell Differentiation drug effects, Chromatography, High Pressure Liquid, Coumaric Acids chemistry, Coumaric Acids isolation & purification, Coumaric Acids metabolism, Coumaric Acids pharmacology, Embryo, Nonmammalian cytology, Embryo, Nonmammalian drug effects, Embryo, Nonmammalian metabolism, Enzyme Inhibitors isolation & purification, Enzyme Inhibitors metabolism, Enzyme Inhibitors pharmacology, Flavonols chemistry, Flavonols isolation & purification, Flavonols metabolism, Flavonols pharmacology, Humans, Melanins metabolism, Monophenol Monooxygenase metabolism, Plant Components, Aerial chemistry, Plant Components, Aerial metabolism, Plants, Medicinal chemistry, Plants, Medicinal metabolism, Spectrometry, Mass, Electrospray Ionization, Tunisia, Zebrafish metabolism, Anti-Inflammatory Agents chemistry, Asteraceae chemistry, Enzyme Inhibitors chemistry, Monophenol Monooxygenase antagonists & inhibitors, Plant Extracts chemistry
- Abstract
The genus Rhanterium (Asteraceae) is a widely distributed medicinal plant throughout western North Africa and some Rhanterium species are used in folk medicine. The aim of research was to investigate methanolic extracts from different parts (flowers, leaves, and stems) of Tunisian Rhanterium suaveolens as potential sources of bioactive products useful for healthy purposes. In particular, were analyzed the phenolic composition of these extracts and their antioxidant, anti-inflammatory, and anti-tyrosinase properties. The phytochemical analyses were performed using standard colorimetric procedures, HPLC-DAD and HPLC-DAD-ESI-MS. Then, several in vitro cell-free assays have been used to estimate the antioxidant/free radical scavenging capability of the extracts. Moreover, in vitro, and in vivo anti-melanogenesis activities of these extracts were tested, respectively, with the tyrosinase inhibition assay and the Zebrafish embryo model. Finally, the anti-inflammatory potential of these extracts in an in vitro model of acute intestinal inflammation in differentiated Caco-2 cells was evaluated. The R. suaveolens extracts under study appeared particularly rich in flavonols and hydroxycinnamic acids and all extracts appeared endowed with good antioxidant/free radical scavenging properties, being the flower extracts slightly more active than the others. Moreover, R. suaveolens flowers extract was able to inhibit in vitro tyrosinase activity and exhibited bleaching effects on the pigmentation of zebrafish embryos. Furthermore, all extracts showed good anti-inflammatory activity in intestinal epithelial cells as demonstrated by the inhibition of TNF-α-induced gene expression of IL-6 and IL-8. R. suaveolens aerial parts may be considered as a potential source of whitening agents, as well as of agents for the treatment of disorders related to oxidative stress and inflammation., (© 2021 The Authors. Chemistry & Biodiversity published by Wiley-VHCA AG, Zurich, Switzerland.)
- Published
- 2021
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