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The reactions of 37 cultivars and two candidate lines of barley to five isolates of Rhynchosporium secalis and their mixtures were determined in assays under greenhouse conditions. Fungal isolates were collected from Ankara province and surrounding areas in Turkey. There were clear differences in the reactions of the cultivars to the fungal isolates and isolates differed in pathogenicity for each cultivar. Of all genotypes tested, seven cultivars (cvs Erginel 90, Şahin 91, Kıral 97, Akhisar 98, Çetin 2000, Çumra 2001, Avcı 2002) and one candidate line (candidate 4) were found to be resistant to all isolates and their mixtures. A5 was found to be the most virulent fungal isolate. [ABSTRACT FROM AUTHOR]