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1. Evaluation of a Low-Cost Method, the Guava EasyCD4 Assay, to Enumerate CD4-Positive Lymphocyte Counts in HIV-lnfected Patients in the United States and Uganda.

Author

Spacek, Lisa A., Shihab, Hasan M., Lutwama, Fred, Summerton, Jean, Mayanja, Harriet, Ronald, Allan, Margolick, Joseph B., Nilles, Tricia L., and Quinn, Thomas C.

Subjects
*IMMUNOASSAY, *IMMUNODIAGNOSIS, *CD4 antigen, *VIRAL receptors, *LYMPHOCYTE receptors, *HIV infections, *HIV-positive persons
Abstract

The article focuses on the evaluation of the low-cost EasyCD4 assay to enumerate CD4 lymphocytes in resource-limited settings in a cross-sectional study conducted in the U.S. and Uganda. The researchers have compated cell counts on replicate samples from HIV-infected patients by the EasyCD4 assay, a microcapillary flow-based system, and by standard flow cytometry or FACSCount with linear regression and the Bland-Altman method. Finally, the results suggest that EasyCD4 may be applied with high positive and negative predictive value in resource-limited settings.

Published
2006
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