1. Genetic diversity of Dirofilaria spp. isolated from subcutaneous and ocular lesions of human patients in Ukraine.
- Author
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Rossi, Alice, Peix, Álvaro, Pavlikovskaya, Tamara, Sagach, Olga, Nikolaenko, Svetlana, Chizh, Nina, Kartashev, Vladimir, Simón, Fernando, and Siles-Lucas, Mar
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DIROFILARIA , *CLADISTIC analysis , *RNA , *BIOLOGICAL variation , *GENE amplification - Abstract
This short communication describes the phylogenetic analysis of 48 Dirofilaria worms isolated from human patients in Ukraine. 102 cases were both of subcutaneous (47; 46.1%) and ocular (54; 52.9%) locations. Worms from 44 patients (15 subcutaneous and 29 ocular) were subjected to DNA extraction and amplification of a specific fragment of the 12S rRNA subunit, and sequences were used for phylogenetic analysis. Results showed that 13.8% of the ocular cases analyzed at molecular level were caused by Dirofilaria immitis . Very few cases of ocular human dirofilariosis due to D. immitis have been described in the literature to date, majority of them attributed to Dirofilaria repens . Our results show that ocular dirofilariosis cannot be excluded in areas of low endemicity for D. repens were D. immitis is also present. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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