1. Rapid molecular detection and genotyping of West Nile Virus lineages 1 and 2 by real time PCR and melting curve analysis.
- Author
-
Faggioni G, De Santis R, Pomponi A, Fantini M, Savini G, Monaco F, Polci A, Bei R, and Lista F
- Subjects
- DNA Primers genetics, Europe epidemiology, Genome, Viral, Oligonucleotide Probes genetics, RNA, Viral genetics, United States epidemiology, West Nile Fever epidemiology, West Nile virus genetics, Genotyping Techniques methods, Real-Time Polymerase Chain Reaction methods, Transition Temperature, West Nile Fever virology, West Nile virus classification, West Nile virus isolation & purification
- Abstract
Following its spread in the USA, West Nile Virus (WNV) has reemerged in the Mediterranean basin with a renewed pathogenicity. The introduction of WNV lineage 2 in Europe and its co-circulation with lineage 1 has resulted in a continuously changing epidemiological scenario, highlighting the importance of differential detection of the two lineages. The paper describes a new real-time PCR method for the detection and genotyping of the two main lineages of WNV. The method requires a single pair of primers and probes and is based on the analysis of highly conserved consensus sequences detected in the 5' terminus of the viral genome., (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF