1. Direct Detection of Pathogens in Bloodstream During Sepsis: Are We There Yet?
- Author
-
Samuel L
- Subjects
- Anti-Infective Agents pharmacology, Anti-Infective Agents therapeutic use, Bacteria genetics, Bacteria isolation & purification, Blood Culture instrumentation, Blood Culture methods, DNA, Bacterial isolation & purification, DNA, Fungal isolation & purification, DNA, Viral isolation & purification, Diagnostic Test Approval, Drug Resistance, Microbial, False Negative Reactions, False Positive Reactions, Fungi genetics, Fungi isolation & purification, Humans, Molecular Diagnostic Techniques instrumentation, Molecular Diagnostic Techniques methods, RNA, Viral isolation & purification, Sensitivity and Specificity, Sepsis blood, Sepsis drug therapy, Sepsis microbiology, United States, United States Food and Drug Administration legislation & jurisprudence, Viruses genetics, Viruses isolation & purification, Blood Culture trends, Molecular Diagnostic Techniques trends, Reagent Kits, Diagnostic, Sepsis diagnosis
- Abstract
Background: Advances in medicine have improved our understanding of sepsis, but it remains a major cause of morbidity and mortality. The detection of pathogens that cause sepsis remains a challenge for clinical microbiology laboratories., Content: Routine blood cultures are time-consuming and are negative in a large proportion of cases, leading to excessive use of broad-spectrum antimicrobials. Molecular testing direct from patient blood without the need for incubation has the potential to fill the gaps in our diagnostic armament and complement blood cultures to provide results in a timely manner. Currently available platforms show promise but have yet to definitively address gaps in sensitivity and specificity., Summary: Significant strides have been made in the detection of pathogens directly from blood. A number of hurdles, however, remain before this technology can be adapted for routine use., (© 2018 American Association for Clinical Chemistry.)
- Published
- 2019
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