1. An international standardization programme towards the application of gene expression profiling in routine leukaemia diagnostics: the Microarray Innovations in LEukemia study prephase.
- Author
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Kohlmann A, Kipps TJ, Rassenti LZ, Downing JR, Shurtleff SA, Mills KI, Gilkes AF, Hofmann WK, Basso G, Dell'orto MC, Foà R, Chiaretti S, De Vos J, Rauhut S, Papenhausen PR, Hernández JM, Lumbreras E, Yeoh AE, Koay ES, Li R, Liu WM, Williams PM, Wieczorek L, and Haferlach T
- Subjects
- Adenocarcinoma genetics, Breast Neoplasms genetics, Carcinoma genetics, Europe, Female, Gene Expression Profiling methods, Humans, Leukemia diagnosis, Liver Neoplasms genetics, Oligonucleotide Array Sequence Analysis methods, Pilot Projects, RNA, Reference Standards, Reproducibility of Results, Singapore, United States, Gene Expression Profiling standards, Leukemia genetics, Oligonucleotide Array Sequence Analysis standards
- Abstract
Gene expression profiling has the potential to enhance current methods for the diagnosis of haematological malignancies. Here, we present data on 204 analyses from an international standardization programme that was conducted in 11 laboratories as a prephase to the Microarray Innovations in LEukemia (MILE) study. Each laboratory prepared two cell line samples, together with three replicate leukaemia patient lysates in two distinct stages: (i) a 5-d course of protocol training, and (ii) independent proficiency testing. Unsupervised, supervised, and r(2) correlation analyses demonstrated that microarray analysis can be performed with remarkably high intra-laboratory reproducibility and with comparable quality and reliability.
- Published
- 2008
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