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The article presents a study which validated the applied biosystems MicroSEQ real-time polymerase chain reaction method to detect Escherichia coli (E. coli) O157:H7 in several good matrixes. The test method was compared to the U.S. Department of Agriculture's "Microbiology Laboratory Guidebook" 5.04 reference method for detecting E. coli in ground beef and beef trim and to the ISO 16654 reference method for detecting E. coli in spinach, orange juice and apple juice.

Elective cosmetic surgeries are increasing in the American population with reasons linked to body image disturbance and body dysmorphic disorder (BDD). Little research exists documenting the continuum of body image disturbance and its relationship to seeking surgery. The present research examined data from 544 participants, 55 of whom were diagnosable with BDD. Using assessments for body image disturbance, problematic coping strategies, and BDD symptomatology, results provided evidence for a continuum of body image distress. Logistic regression analysis supported the hypothesis that increased levels of body image disturbance and one type of problematic coping strategy (Appearance Fixing) predicted consideration of cosmetic surgery. Of participants diagnosable with BDD, those who considered cosmetic surgery showed more severe body image disturbance and problematic coping than those who did not consider surgery. These results have implications for pre-surgical assessment as well as psychological interventions rather than invasive medical interventions., (Copyright © 2011 Elsevier Ltd. All rights reserved.)