1. Biomimetic microbioreactor-supramolecular nanovesicles improve enzyme therapy of hepatic cancer.
- Author
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He, Xiaoqian, Feng, Jiao, Yan, Shenglei, Zhang, Yonghong, Zhong, Cailing, Liu, Yuying, Shi, Da, Abagyan, Ruben, Xiang, Tingxiu, and Zhang, Jingqing
- Subjects
ARGININE deiminase ,CANCER treatment ,LIVER cells ,ENZYMES ,POLYETHYLENE glycol ,CYCLODEXTRIN derivatives - Abstract
A novel biomimetic nanovesicle-loaded supramolecular enzyme-based therapeutics has been developed. Here, using a biomimetic lipid-D-α-tocopherol polyethylene glycol succinate (TPGS) hybrid semi-permeable membrane, cyclodextrin supramolecular docking, metal-ion-aided coordination complexing, we combined multiple functional motifs into a single biomimetic microbioreactor-supramolecular nanovesicle (MiSuNv) that allowed effective transport of arginine deiminase (ADI) to hepatic tumor cells to enhance arginine depletion. We compared two intercalated enzyme-carrying supermolecular motifs mainly comprising of 2-hydroxypropyl-β-cyclodextrin and sulfobutyl-ether-β-cyclodextrin, the only two cyclodextrin derivatives approved for injection by the United States Food and Drug Administration. The ADI-specific antitumor effects were enhanced by TPGS (one constituent of MiSuNv, having synergistic antitumor effects), as ADI was separated from adverse external environment by a semi-permeable membrane and sequestered in a favorable internal microenvironment with an optimal pH and metal-ion combination. ADI@MiSuNv contributed to cell cycle arrest, apoptosis and autophagy through the enhanced efficacy of enzyme treatment against Hep3B xenograft tumors in rats. Using a biomimetic lipid-D-α-tocopherol polyethylene glycol succinate hybrid semi-permeable membrane, cyclodextrin supramolecular docking, metal-ion-aided coordination complexing, we combined the multiple functional motifs into a single biomimetic microbioreactor-supramolecular nanovesicle that allowed effective transport of arginine deiminase to hepatic tumor cells to enhance arginine depletion. Unlabelled Image [ABSTRACT FROM AUTHOR]
- Published
- 2021
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