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Serial crystallography is a powerful technique in structure determination using many small crystals at X‐ray free‐electron laser or synchrotron radiation facilities. The large diffraction data volumes require high‐throughput software to preprocess the raw images for subsequent analysis. ClickX is a program designated for serial crystallography data preprocessing, capable of rapid data sorting for online feedback and peak‐finding refinement by parameter optimization. The graphical user interface (GUI) provides convenient access to various operations such as pattern visualization, statistics plotting and parameter tuning. A batch job module is implemented to facilitate large‐data‐volume processing. A two‐step geometry calibration for single‐panel detectors is also integrated into the GUI, where the beam center and detector tilting angles are optimized using an ellipse center shifting method first, then all six parameters, including the photon energy and detector distance, are refined together using a residual minimization method. Implemented in Python, ClickX has good portability and extensibility, so that it can be installed, configured and used on any computing platform that provides a Python interface or common data file format. ClickX has been tested in online analysis at the Pohang Accelerator Laboratory X‐ray Free‐Electron Laser, Korea, and the Linac Coherent Light Source, USA. It has also been applied in post‐experimental data analysis. The source code is available via https://github.com/LiuLab‐CSRC/ClickX under a GNU General Public License. [ABSTRACT FROM AUTHOR]