1. Virome of crab-eating (Cerdocyon thous) and pampas foxes (Lycalopex gymnocercus) from southern Brazil and Uruguay.
- Author
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Weber MN, Mosena ACS, da Silva MS, Canova R, de Lorenzo C, Olegário JC, Budaszewski RF, Baumbach LF, Soares JF, Sonne L, Varela APM, Mayer FQ, de Oliveira LGS, and Canal CW
- Subjects
- Anelloviridae classification, Anelloviridae genetics, Animals, Bocavirus classification, Bocavirus genetics, Brazil, DNA Viruses classification, DNA Viruses genetics, DNA, Viral, Distemper Virus, Canine classification, Distemper Virus, Canine genetics, High-Throughput Nucleotide Sequencing, Lymph Nodes virology, Metagenomics, Paramyxoviridae classification, Paramyxoviridae genetics, Parvoviridae classification, Parvoviridae genetics, Parvovirus, Canine classification, Parvovirus, Canine genetics, Phylogeny, RNA, Viral, Spleen virology, Uruguay, Virus Diseases veterinary, Virus Diseases virology, Viruses isolation & purification, Dogs virology, Foxes virology, Virome, Viruses classification, Viruses genetics
- Abstract
Crab-eating (Cerdocyon thous) and Pampas foxes (Lycalopex gymnocercus) are wild canids distributed in South America. Domestic dogs (Canis lupus familiaris) and wild canids may share viral pathogens, including rabies virus (RABV), canine distemper virus (CDV), and canine parvovirus 2 (CPV-2). To characterize the virome of these wild canid species, the present work evaluated the spleen and mesenteric lymph node virome of 17 crab-eating and five Pampas foxes using high-throughput sequencing (HTS). Organ samples were pooled and sequenced using an Illumina MiSeq platform. Additional PCR analyses were performed to identify the frequencies and host origin for each virus detected by HTS. Sequences more closely related to the Paramyxoviridae, Parvoviridae and Anelloviridae families were detected, as well as circular Rep-encoding single-stranded (CRESS) DNA viruses. CDV was found only in crab-eating foxes, whereas CPV-2 was found in both canid species; both viruses were closely related to sequences reported in domestic dogs from southern Brazil. Moreover, the present work reported the detection of canine bocavirus (CBoV) strains that were genetically divergent from CBoV-1 and 2 lineages. Finally, we also characterized CRESS DNA viruses and anelloviruses with marked diversity. The results of this study contribute to the body of knowledge regarding wild canid viruses that can potentially be shared with domestic canids or other species., (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Published
- 2020
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