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The relationship between the type of agar used to prepare solid nutrient media and the growth of Bl. trispora strains (+)8A and (-)8A was under study. Bl. trispora culture virtually failed to grow in the media prepared with powder agar, GOST 16280-70: it died after 2-month storage with 2 reinoculations. Bl. trispora grew fairly well in culture media with white frozen lamellar agar (GOST 16280-70) or with white frozen microbiologic agar, GOST 17206-71; the characteristic features of the culture were preserved after 10 monthly reinoculations.