1. Variations in the core promoter/pre-core region in HBV genotype C in Japanese and Northern Vietnamese patients.
- Author
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Truong BX, Yano Y, Seo Y, Phuong TM, Tanaka Y, Kato H, Miki A, Utsumi T, Azuma T, Trach NK, Mizokami M, Hayashi Y, and Kasuga M
- Subjects
- Adult, Carcinoma, Hepatocellular etiology, Cross-Sectional Studies, DNA, Viral blood, Female, Genotype, Hepatitis B complications, Hepatitis B virology, Humans, Japan epidemiology, Liver Cirrhosis etiology, Liver Neoplasms etiology, Male, Middle Aged, Molecular Epidemiology, Phylogeny, Polymorphism, Restriction Fragment Length, Risk Factors, Vietnam epidemiology, Hepatitis B epidemiology, Hepatitis B genetics, Hepatitis B e Antigens blood, Hepatitis B virus genetics, Promoter Regions, Genetic
- Abstract
Hepatitis B virus (HBV) subgenotypes Cs (C1) and Ce (C2) are common in East Asia. To investigate the genomic difference of HBV genotype C between two separated regions, 50 subgenotype Cs-infected Vietnamese and 70 subgenotype Ce-infected Japanese patients were enrolled for analysis. The patients were categorized to either a hepatocellular carcinoma group (HCC) or a non-HCC group including liver cirrhosis, chronic hepatitis, and asymptomatic carriers. HBV serology, HBV-DNA level, and variations in core promoter/pre-core region were examined. Phylogenetic analysis based on the full genome sequences and nucleotide sequences partly in the S gene and in the P gene revealed that all Japanese strains (70/70) were subgenotype Ce, and nearly all of the Vietnamese strains (50/51) were subgenotype Cs, excluding one subgenotype C5. C1858 and G1775 were common in the Vietnamese (64% and 40%) but not in the Japanese (0%). The prevalence of C/A1753 in Vietnamese was higher than that in the Japanese (32% vs. 17.1%), however the frequency of A1896 in the Japanese was significantly higher (32.9% vs. 12%, P < 0.05). Most of the Vietnamese patients with HCC had a high level of HBV-DNA, the Japanese HCC had a relatively low level. In the Vietnamese, C/A1753 and C1858 were associated closely with T1762A1764, higher HBV-DNA levels and higher HCC incidence. The multivariate analysis revealed that male, T1653 and C/A1753 were independent risk factors for HCC. The subgenotypes and unique mutations of HBV genotype C in the Vietnamese and Japanese differed, and C/A1753 and C1858 variants might play a role in the pathogenesis of liver disease in Vietnamese patients.
- Published
- 2007
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