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Porcine epidemic diarrhea (PED) is characterized by acute enteritis, watery diarrhea, weight loss, dehydration, and death, with high mortality in neonatal piglets. In this study, 3 virus isolates collected in Vietnam between 2016 and 2017 were propagated successfully in Vero cells at high virus titers. Sequence analysis of the fulllength spike (S) gene showed that all 3 isolates belong to genogroup 2b, which is closely related to other prevalent Asian strains. A comparison of the amino acid sequence revealed a 98.19% to 99.13% homology with the Vietnam isolates circulating during 2013–2015, suggesting that field PED viruses (PEDVs) are evolving continuously. Experiments in animals showed that the antisera from guinea pigs immunized with the vaccine strain resulted in higher levels (5 log2) of neutralizing antibodies against the homologous strain and a relatively moderate level of neutralizing antibodies against the field isolates. This finding would be helpful in selecting a PEDV strain for vaccine development. [ABSTRACT FROM AUTHOR]

Background: While early detection and early containment are key to controlling the African swine fever (ASF) pandemic, the lack of practical testing methods for use in the field are a major barrier to achieving this feat. Objectives: To describe the development of a rapid and sensitive point‐of‐care test (POCT) for ASF, and its evaluation using swine whole blood samples for field settings. Methods: In total, 89 swine whole blood samples were collected from Vietnamese swine farms and were performed the POCT using a combination of crude DNA extraction and LAMP (loop‐mediated isothermal amplification) amplification. Results: The POCT enabled crude DNA to be extracted from swine whole blood samples within 10 min at extremely low cost and with relative ease. The entire POCT required a maximum of 50 min from the beginning of DNA extraction to final judgment. Compared to a conventional real‐time PCR detection, the POCT showed a 1 log reduction in detection sensitivity, but comparable diagnostic sensitivity of 100% (56/56) and diagnostic specificity of 100% (33/33). The POCT was quicker and easier to perform and did not require special equipment. Conclusions: This POCT is expected to facilitate early diagnosis and containment of ASF invasion into both regions in which it is endemic and eradicated. [ABSTRACT FROM AUTHOR]

Investigation of the role of animals that have recovered and survived from African swine fever (ASF) in carrying the ASF virus is currently intense and ongoing. However, no clear definition of the carrier stage has been established. The aim of the present study was to establish criteria to elucidate a clear status of survival in naturally ASF-infected domestic pigs in Vietnam. Seroconversion from previous infection was confirmed by serological assay, and the absence of the viral genome in various organs was also assured by molecular analysis of a partial p72 gene. We recognized that histopathological evidence could benefit from further insights into the status and role of the surviving animals; therefore, we performed a histopathological study on four pigs from farms with a history of ASF outbreak. We found fibrotic changes in the reparative process as the main finding in all four pigs. Immunohistochemical detection of viral protein revealed an interesting result. Despite the negative result from viral genome detection, the p30 protein gave a positive signal in the tonsils, lung, and stomach. This raises the possibility of stress-induced viral reactivation in long-term survivors and the risk of further outbreaks from human handling of contaminated carcasses. [ABSTRACT FROM AUTHOR]

Porcine epidemic diarrhea (PED) is characterized by acute enteritis, watery diarrhea, weight loss, dehydration, and death with high mortality in neonatal piglets. In this study, 3 virus isolates collected in Vietnam between 2016 and 2017 were successfully propagated in Vero cells at high virus titers. Sequence analysis of the full-length spike (S) gene revealed that all 3 isolates belong to genogroup 2a, which is closely related to other prevalent Asian strains. Amino acid sequence comparisons revealed 98.19% to 99.13% homology with the Vietnam isolates circulating during 2013–2015, suggesting that field PED viruses (PEDVs) evolve continuously. Experiments in animals demonstrated that antisera from guinea pigs immunized with the vaccine strain resulted in higher levels (5 log2) of neutralizing antibody against the homologous strain, and showed a relatively lower level of neutralizing antibody against the field isolates. This finding would be helpful in choosing a PEDV strain for vaccine development. [ABSTRACT FROM AUTHOR]

BACKGROUND Siblings play an important role in a child's life. However, many children often experience sibling bullying. This study investigates differences in sibling victimization by sex, age, a parent's absence from the home due to employment, or a child's privacy and the relationship between sibling victimization, peer victimization, and the child's well-being. PARTICIPANTS AND PROCEDURE Participants were Vietnamese children participating in the third wave of the International Survey of Children's Well-Being. The study included 1537 children (811 boys and 726 girls) attending public schools, age 10-14 years (M = 11.29, SD = 1.15). RESULTS. The results show that over half of children with siblings in this study reported being victimized by a sibling. Younger children were bullied more often than older children. Children whose father worked away from home reported an increase in bullying behavior from their siblings. Children sharing a room with siblings reported being bullied more by siblings. CONCLUSIONS The results indicated a positive correlation between sibling victimization and peer victimization and a negative relationship between being bullied and a child's subjective well-being. [ABSTRACT FROM AUTHOR]