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1. Detection of Salmonella strains and Escherichia coli O157:H7 in feces of small ruminants and their isolation with various media.

Author

Pao S, Patel D, Kalantari A, Tritschler JP, Wildeus S, and Sayre BL

Subjects
Animals, Bacteriological Techniques, Goat Diseases microbiology, Goats, Immunoenzyme Techniques, Male, Salmonella typhimurium isolation & purification, Sheep, Sheep Diseases microbiology, Virginia, Culture Media, Escherichia coli Infections veterinary, Escherichia coli O157 isolation & purification, Feces microbiology, Ruminants microbiology, Salmonella isolation & purification, Salmonella Infections, Animal microbiology
Abstract

Salmonella strains and Escherichia coli O157:H7 were detected in 17 and 5 small ruminants in Virginia, respectively, of 287 tested. Background microflora interfered with the fecal analysis. The combination of Salmonella enzyme immunoassay (EIA) detection and xylose-lysine-deoxycholate agar isolation was satisfactory. Modifying enrichment to a 1:100 dilution enabled effective E. coli O157:H7 detection by EIA and isolation by sorbitol-MacConkey agar with cefixime-tellurite.

Published
2005
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