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In the brackish water sedimentary environment of the Junggar Basin in Xinjiang, the organic-rich shale and shale oil formation with relatively low maturity, is characterized by relatively high abundance of C 28 regular steranes. However, the biogenic source of organic matter and favorable sedimentary environment for the type of source rock has been thoroughly classified. In this paper, organic geochemical and petrological, analyses of the Lucaogou Formation shale in the Jimusaer Sag, Junggar Basin in the north of Xinjiang Uygur Autonomy Region were carried out. The source rocks in the Lucaogou Formation are mainly composed of two types of maceral including lamalginite (cyanobacteria) and telalginite (green algae), with the former predominantly fueling the high C 28 regular sterane content. Petrological, organic and inorganic geochemical analyses suggest that the lamalginite was developed in low-salinity water while the telalginite was developed in high-salinity water. Based on geochemical and organic petrological characteristics, both the lamalginite and telalginite have good hydrocarbon-generating potential, but in the lower thermal evolution stage of the Lucaogou Formation source rock, telalginite have higher hydrocarbon-generating rate. • Lamalginite (cyanobacteria) is the primary source of C 28 regular steranes. • The lamalginite (cyanobacteria) is more developed when the salinity is low and the telalginite (green algae) is more developed when the salinity is high. • In the lower thermal evolution stage of the Lucaogou Formation source rock, the lamalginite (cyanobacteria) has lower hydrocarbon-generating rate than the telalginite (green algae). [ABSTRACT FROM AUTHOR]