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2. Persistent carriage of subpatent Plasmodium falciparum parasites associated with clinical malaria in a low transmission area in Senegal

Author

Sambe, Babacar Souleymane, Sarr, Ibrahima, Diagne, Aissatou, Diatta, Arona Sabène, Faye, Joseph, Diagne, Nafissatou, Diaw, Serigne Ousmane Mbacké, Mbodj, Adja Fatou, Sané, Rokhaya, Wotodjo, Amélé Nyedzie, Diouf, Babacar, Thiam, Alassane, Diamanka, Arfang, Faye, Ngor, Sembène, Papa Mbacké, Sarr, Fatoumata Diene, Dia, Ibrahima, Vigan-Womas, Inès, Sokhna, Cheikh, Toure-Balde, Aissatou, and Niang, Makhtar

Published
2024
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3. One hundred malaria attacks since birth. A longitudinal study of African children and young adults exposed to high malaria transmission

Author

Trape, Jean-François, Diagne, Nafissatou, Diene-Sarr, Fatoumata, Faye, Joseph, Dieye-Ba, Fambaye, Bassène, Hubert, Badiane, Abdoulaye, Bouganali, Charles, Tall, Adama, Ndiaye, Ramatoulaye, Doucouré, Souleymane, Wotodjo, Amélé Nyedzie, Vigan-Womas, Inès, Guillotte-Blisnick, Micheline, Talla, Cheikh, Niang, Makhtar, Touré-Baldé, Aissatou, Perraut, Ronald, Roussilhon, Christian, Druilhe, Pierre, Rogier, Christophe, Mercereau-Puijalon, Odile, Loucoubar, Cheikh, and Sokhna, Cheikh

Published
2024
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10. Molecular detection and quantification of Plasmodium vivax DNA in blood pellet and plasma samples from patients in Senegal

Author

Sambe, Babacar Souleymane, primary, Diagne, Aissatou, additional, Diatta, Hélène Ataume Mawounge, additional, Gaba, Folly Mawulolo, additional, Sarr, Ibrahima, additional, Diatta, Arona Sabène, additional, Diaw, Serigne Ousmane Mbacké, additional, Sané, Rokhaya, additional, Diouf, Babacar, additional, Vigan-Womas, Inès, additional, Mbengue, Babacar, additional, and Niang, Makhtar, additional

Published
2023
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16. A switch in infected erythrocyte deformability at the maturation and blood circulation of Plasmodium falciparum transmission stages

Author

Tibúrcio, Marta, Niang, Makhtar, Deplaine, Guillaume, Perrot, Sylvie, Bischoff, Emmanuel, Ndour, Papa Alioune, Silvestrini, Francesco, Khattab, Ayman, Milon, Geneviève, David, Peter H., Hardeman, Max, Vernick, Kenneth D., Sauerwein, Robert W., Preiser, Peter R., Mercereau-Puijalon, Odile, Buffet, Pierre, Alano, Pietro, and Lavazec, Catherine

Published
2012
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17. Correction to: Fine-scale Spatiotemporal Mapping of Asymptomatic and Clinical Plasmodium falciparum Infections: Epidemiological Evidence for Targeted Malaria Elimination Interventions

Author

Niang, Makhtar, primary, Sandfort, Mirco, additional, Mbodj, Adja Fatou, additional, Diouf, Babacar, additional, Talla, Cheikh, additional, Faye, Joseph, additional, Sane, Rokhaya, additional, Thiam, Laty Gaye, additional, Thiam, Alassane, additional, Badiane, Abdoulaye, additional, Vigan-Womas, Ines, additional, Diagne, Nafissatou, additional, Diene Sarr, Fatoumata, additional, Mueller, Ivo, additional, Sokhna, Cheikh, additional, White, Michael, additional, and Toure-Balde, Aissatou, additional

Published
2022
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20. Feature article: Cell trace far-red is a suitable erythrocyte dye for multi-color Plasmodium falciparum invasion phenotyping assays

Author

Thiam, Laty G, Aniweh, Yaw, Quansah, Evelyn B, Donkor, Jacob K, Gwira, Theresa M, Kusi, Kwadwo A, Niang, Makhtar, and Awandare, Gordon A

Subjects
Erythrocytes, Time Factors, Staining and Labeling, Plasmodium falciparum, Color, Flow Cytometry, Fluorescence, Phenotype, Animals, Humans, Biological Assay, Parasites, Coloring Agents, Original Research
Abstract

Plasmodium falciparum erythrocyte invasion phenotyping assays are a very useful tool for assessing parasite diversity and virulence, and for characterizing the formation of ligand–receptor interactions. However, such assays need to be highly sensitive and reproducible, and the selection of labeling dyes for differentiating donor and acceptor erythrocytes is a critical factor. We investigated the suitability of cell trace far-red (CTFR) as a dye for P. falciparum invasion phenotyping assays. Using the dyes carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) and dichloro dimethyl acridin one succinimidyl ester (DDAO-SE) as comparators, we used a dye-dilution approach to assess the limitations and specific staining procedures for the applicability of CTFR in P. falciparum invasion phenotyping assays. Our data show that CTFR effectively labels acceptor erythrocytes and provides a stable fluorescent intensity at relatively low concentrations. CTFR also yielded a higher fluorescence intensity relative to DDAO-SE and with a more stable fluorescence intensity over time. Furthermore, CTFR did not affect merozoites invasion of erythrocytes and was not toxic to the parasite’s intraerythrocytic development. Additionally, CTFR offers flexibility in the choice of combinations with several other DNA dyes, which broaden its usage for P. falciparum erythrocyte invasion assays, considering a wider range of flow cytometers with various laser settings. IMPACT STATEMENT: In recent years, flow cytometry has become a cornerstone in investigating P. falciparum phenotypic diversity using multiple dyes to discriminate between donor and acceptor erythrocytes. To broaden the applicability of such assays, we optimized the staining conditions of a newly developed cytoplasmic dye, cell trace far-red (CTFR), and assessed its suitability for use in P. falciparum invasion phenotyping assays. We showed that CTFR has a very narrow emission peak excited by red lasers. Furthermore, CTFR labeling of target erythrocytes, achieved even at low concentrations, is stable over time and did not impair parasite development. P. falciparum erythrocyte invasion phenotyping assays revealed that CTFR is suitable for use in combination with several DNA dyes in multiplex assays. This will allow for high throughput phenotyping of parasites as well as facilitate the evaluation of preference of erythrocytes by merozoites. Altogether, these make screening for potential invasion-blocking interventions possible.

Published
2020

21. Fine-scale Spatiotemporal Mapping of Asymptomatic and Clinical Plasmodium falciparum Infections: Epidemiological Evidence for Targeted Malaria Elimination Interventions

Author

Niang, Makhtar, primary, Sandfort, Mirco, additional, Mbodj, Adja Fatou, additional, Diouf, Babacar, additional, Talla, Cheikh, additional, Faye, Joseph, additional, Sane, Rokhaya, additional, Thiam, Laty Gaye, additional, Thiam, Alassane, additional, Badiane, Abdoulaye, additional, Vigan-Womas, Ines, additional, Diagne, Nafissatou, additional, Diene Sarr, Fatoumata, additional, Mueller, Ivo, additional, Sokhna, Cheikh, additional, White, Michael, additional, and Toure-Balde, Aissatou, additional

Published
2021
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22. Cross-Reactivity of SARS-CoV-2 Laboratory Diagnostics to Endemic Diseases in Africa: A Diagnostic Accuracy Study

Author

Diagne, Cheikh Tidiane, primary, Ndiaye, Oumar, additional, Talla, Cheikh, additional, Dia, Fatou, additional, Faye, Rokhaya, additional, Diouf, Babacar, additional, Tall, Billo, additional, Mbengue, Alassane, additional, Diop, Mamadou, additional, Diallo, Khardiata, additional, Cisse, Vivianne Marie Pierre, additional, Ndoye, Amadou Moustapha, additional, Barry, Aliou, additional, Niang, Makhtar, additional, Loucoubar, Cheikh, additional, Dia, Ndongo, additional, Bei, Amy K., additional, Fitchett, Joe, additional, Taieb, Fabien, additional, Faye, Ousmane, additional, Peeling, Rosanna W., additional, Vigan-Womas, Inés, additional, and Sall, Amadou, additional

Published
2021
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23. Resistance of Plasmodium falciparum field isolates to in-vitro artemether and point mutations of the SERCA-type PfATPase6

Author

Jambou, Ronan, Legrand, Eric, Niang, Makhtar, Khim, Nimol, Lim, Pharath, Volney, Beatrice, Ekala, Marie Therese, Bouchier, Christiane, Esterre, Philippe, Fandeur, Thierry, and Mercereau-Puijalan, Odile

Subjects
Malaria -- Drug therapy, Artemisia -- Research, Drug resistance -- Research, Antimalarials -- Dosage and administration, Antimalarials -- Evaluation
Published
2005

24. Field evaluation of a mobile biosafety laboratory in Senegal to strengthen rapid disease outbreak response and monitoring

Author

Fall, Cheikh, primary, Cappuyns, Aurélie, additional, Faye, Oumar, additional, Pauwels, Steven, additional, Fall, Gamou, additional, Dia, Ndongo, additional, Diagne, Moussa M., additional, Diagne, Cheikh T., additional, Niang, Makhtar, additional, Mbengue, Alassane, additional, Faye, Martin, additional, Dieng, Idrissa, additional, Gningue, Babacar, additional, Bousso, Abdoulaye, additional, Faye, Ousmane, additional, Pauwels, Rudi, additional, and Sall, Amadou A., additional

Published
2020
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25. Immune tolerance to asymptomatic submicroscopic Plasmodium falciparum infections in adults living in malaria endemic areas

Author

Diouf, Babacar BD, primary, Diop, Fode, additional, Dieye, Yakhya, additional, Mbodji, Adja Fatou, additional, Talla, Cheikh, additional, Thiam, Alassane, additional, Dia, Ibrahima, additional, Faye, Joseph, additional, Sembène, Mbacké, additional, Vigan-Womas, Inès, additional, Sarr, Fatoumata Diène, additional, Perraut, Ronald, additional, Toure-Balde, Aïssatou, additional, and Niang, Makhtar, additional

Published
2020
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31. Low genetic diversity and complexity of submicroscopic Plasmodium falciparum infections among febrile patients in low transmission areas in Senegal

Author

Sane, Rokhaya, primary, Talla, Cheikh, additional, Diouf, Babacar, additional, Sarr, Fatoumata Diene, additional, Diagne, Nafissatou, additional, Faye, Joseph, additional, Badiane, Abdoulaye, additional, Sembène, Pape Mbacké, additional, Sokhna, Cheikh, additional, Toure-Balde, Aissatou, additional, and Niang, Makhtar, additional

Published
2019
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33. Chronic infection during placental malaria is associated with up-regulation of cycloxygenase-2

Author

Niang Makhtar, Brahim Hamoud O, Gaye Alioune, Frealle Emilie, Marrama Laurence, Aldebert Delphine, Sarr Demba, Dangou Jean, Mercereau-Puijalon Odile, Lehesran Jean, and Jambou Ronan

Subjects
Arctic medicine. Tropical medicine, RC955-962, Infectious and parasitic diseases, RC109-216
Abstract

Abstract Background Placental malaria (PM) is associated with poor foetal development, but the pathophysiological processes involved are poorly understood. Cyclooxygenase (COX) and lipoxygenase (LOX) which convert fatty acids to prostaglandins and leukotrienes, play important roles in pregnancy and foetal development. COX-2, currently targeted by specific drugs, plays a dual role as it associates with both pre-eclampsia pathology and recovery during infection. The role of COX during PM was questioned by quantifying at delivery COX-1, COX-2, 15-LOX, and IL-10 expression in two groups of malaria infected and uninfected placenta. Methods Placental biopsies were collected at delivery for mRNA isolation and quantification, using real time PCR. Results COX-2 and IL-10 mRNAs increased mainly during chronic infections (nine- and five-times, respectively), whereas COX-1 transcripts remained constant. COX-2 over-expression was associated with a higher birth weight of the baby, but with a lower rate of haemoglobin of the mother. It was associated with a macrophage infiltration of the placenta and with a low haemozoin infiltration. In the opposite way, placental infection was associated with lower expression of 15-LOX mRNA. A high degree of haemozoin deposition correlates with low birth weight and decreased expression of COX-2. Conclusion These data provide evidence that COX-2 and IL-10 are highly induced during chronic infection of the placenta, but were not associated with preterm delivery or low birth weight. The data support the involvement of COX-2 in the recovery phase of the placental infection.

Published
2010
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34. Characterization of the repertoire diversity of the Plasmodium falciparum stevor multigene family in laboratory and field isolates

Author

Holder Anthony A, Marsh Kevin, Niang Makhtar, Blythe Jane E, Langhorne Jean, and Preiser Peter R

Subjects
Arctic medicine. Tropical medicine, RC955-962, Infectious and parasitic diseases, RC109-216
Abstract

Abstract Background The evasion of host immune response by the human malaria parasite Plasmodium falciparum has been linked to expression of a range of variable antigens on the infected erythrocyte surface. Several genes are potentially involved in this process with the var, rif and stevor multigene families being the most likely candidates and coding for rapidly evolving proteins. The high sequence diversity of proteins encoded by these gene families may have evolved as an immune evasion strategy that enables the parasite to establish long lasting chronic infections. Previous findings have shown that the hypervariable region (HVR) of STEVOR has significant sequence diversity both within as well as across different P. falciparum lines. However, these studies did not address whether or not there are ancestral stevor that can be found in different parasites. Methods DNA and RNA sequences analysis as well as phylogenetic approaches were used to analyse the stevor sequence repertoire and diversity in laboratory lines and Kilifi (Kenya) fresh isolates. Results Conserved stevor genes were identified in different P. falciparum isolates from different global locations. Consistent with previous studies, the HVR of the stevor gene family was found to be highly divergent both within and between isolates. Importantly phylogenetic analysis shows some clustering of stevor sequences both within a single parasite clone as well as across different parasite isolates. Conclusion This indicates that the ancestral P. falciparum parasite genome already contained multiple stevor genes that have subsequently diversified further within the different P. falciparum populations. It also confirms that STEVOR is under strong selection pressure.

Published
2009
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36. Modelling dynamic change of malaria transmission in holoendemic setting (Dielmo, Senegal) using longitudinal measures of antibody prevalence to Plasmodium falciparum crude schizonts extract

Author

Niass, Oumy, primary, Saint-Pierre, Philippe, additional, Niang, Makhtar, additional, Diop, Fode, additional, Diouf, Babacar, additional, Faye, Michel Matar, additional, Sarr, Fatoumata Diène, additional, Faye, Joseph, additional, Diagne, Nafissatou, additional, Sokhna, Cheikh, additional, Trape, Jean-François, additional, Perraut, Ronald, additional, Tall, Adama, additional, Diongue, Abdou Kâ, additional, and Toure Balde, Aïssatou, additional

Published
2017
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37. Substantial asymptomatic submicroscopic Plasmodium carriage during dry season in low transmission areas in Senegal: Implications for malaria control and elimination

Author

Niang, Makhtar, primary, Thiam, Laty Gaye, additional, Sane, Rokhaya, additional, Diagne, Nafissatou, additional, Talla, Cheikh, additional, Doucoure, Souleymane, additional, Faye, Joseph, additional, Diop, Fode, additional, Badiane, Abdoulaye, additional, Diouf, Babacar, additional, Camara, Diogop, additional, Diene-Sarr, Fatoumata, additional, Sokhna, Cheikh, additional, Richard, Vincent, additional, and Toure-Balde, Aissatou, additional

Published
2017
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38. Temporal analysis of IgG antibody responses to Plasmodium falciparum antigens in relation to changing malaria epidemiology in a West African setting

Author

Niang, Makhtar, primary, Niass, Oumy, additional, Diagne, Nafissatou, additional, Sarr, Fatoumata Diene, additional, Faye, Michel Matar, additional, Diop, Fode, additional, Diouf, Babacar, additional, Faye, Joseph, additional, Badiane, Abdoulaye, additional, Perraut, Ronald, additional, Sokhna, Cheikh, additional, Trape, Jean-François, additional, Tall, Adama, additional, and Toure-Balde, Aissatou, additional

Published
2017
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40. The variant STEVOR protein of Plasmodium falciparum is a red cell binding protein important for merozoite invasion and rosetting

Author

Niang, Makhtar, Bei, Amy Kristine, Madnani, Kripa Gopal, Pelly, Shaaretha, Dankwa, Selasi, Kanjee, Usheer, Gunalan, Karthigayan, Amaladoss, Anburaj, Yeo, Kim Pin, Bob, Ndeye Sakha, Malleret, Benoit, Duraisingh, Manoj, and Preiser, Peter Rainer

Subjects
Erythrocytes, Merozoites, Virulence Factors, Plasmodium falciparum, Protozoan Proteins, Antigens, Protozoan, Receptors, Cell Surface, Article, parasitic diseases, COS Cells, Chlorocebus aethiops, Host-Pathogen Interactions, Animals, Humans, Glycophorins
Abstract

Variant surface antigens play an important role in Plasmodium falciparum malaria pathogenesis and in immune evasion by the parasite. Although most work to date has focused on P. falciparum Erythrocyte Membrane Protein 1 (PfEMP1), two other multigene families encoding STEVOR and RIFIN are expressed in invasive merozoites and on the infected erythrocyte surface. However, their role during parasite infection remains to be clarified. Here we report that STEVOR functions as an erythrocyte-binding protein that recognizes Glycophorin C (GPC) on the red blood cell (RBC) surface and that its binding correlates with the level of GPC on the RBC surface. STEVOR expression on the RBC leads to PfEMP1-independent binding of infected RBCs to uninfected RBCs (rosette formation), while antibodies targeting STEVOR in the merozoite can effectively inhibit invasion. Our results suggest a PfEMP1-independent role for STEVOR in enabling infected erythrocytes at the schizont stage to form rosettes and in promoting merozoite invasion.

Published
2014

42. A molecular survey of acute febrile illnesses reveals Plasmodium vivax infections in Kedougou, southeastern Senegal

Author

Niang, Makhtar, primary, Thiam, Laty Gaye, additional, Sow, Abdourahmane, additional, Loucoubar, Cheikh, additional, Bob, Ndeye Sakha, additional, Diop, Fode, additional, Diouf, Babacar, additional, Niass, Oumy, additional, Mansourou, Annick, additional, Varela, Marie Louise, additional, Perraut, Ronald, additional, Sall, Amadou A, additional, and Toure-Balde, Aissatou, additional

Published
2015
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44. Three Is a Crowd - New Insights into Rosetting in Plasmodium falciparum.

Author

Xue Yan Yam, Niang, Makhtar, Madnani, Kripa Gopal, and Preiser, Peter R.

Subjects
*PLASMODIUM falciparum, *PARASITES, *IMMUNE response, *ANTIGENS, *HOMEOSTASIS, *ERYTHROCYTES
Abstract

The intracellular malaria parasites extensively modify host erythrocytes to allow nutrient uptake, ensure homeostasis, and evade the host's immune response. To achieve this, the parasite exports several proteins to the erythrocyte surface. In Plasmodium falciparum, the parasite responsible for the most severe form of human malaria, three major variant surface antigen families - PfEMP1, STEVOR, and RIFIN - have been implicated in contributing to immune evasion, parasite sequestration, and parasite-mediated rosetting of uninfected erythrocytes. Sequestration and rosetting have been linked to parasite-mediated pathology, making the variant surface antigens of P. falciparum major virulence factors. Here we review our current understanding of rosetting mechanism, recent findings of STEVOR, RIFIN-mediated rosetting, and their implication on the severity and pathology of the disease. [ABSTRACT FROM AUTHOR]

Published
2017
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45. Asymptomatic Plasmodium vivaxinfections among Duffy-negative population in Kedougou, Senegal

Author

Niang, Makhtar, Sane, Rokhaya, Sow, Abdourahmane, Sadio, Bacary, Chy, Sophy, Legrand, Eric, Faye, Ousmane, Diallo, Mawlouth, Sall, Amadou, Menard, Didier, and Toure-Balde, Aissatou

Abstract

In the southeastern Senegal, the report of Plasmodium vivaxinfections among febrile patients in Kedougou constitutes a new emerging health problem. Samples from 48 asymptomatic schoolchildren sampled twice a year over 2 years were used to explore the reservoir of P. vivaxparasite infections in this region. Both Duffy genotyping and Plasmodiumspecies diagnostic assays were performed. PCR assays detected Plasmodiumgenomic DNA in 38.5% (74/192) of samples. Pure P. falciparumand P. vivaxinfections were identified in 79.7% (59/74) and 20.3% (15/74) of samples, respectively. All schoolchildren were classified as Duffy-negative by genotyping. P. vivaxinfections were detected in five children: in two children during both years, in one child in 2010 and on May 2011, and only in 2010 for the remaining two children. This unexpectedly high proportion of P. vivaxinfections in asymptomatic Duffy-negative children highlights to consider vivax malaria as an emerging problem in Senegal.

Published
2018
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48. Geographic Structuring of the Plasmodium falciparum Sarco(endo)plasmic Reticulum Ca2+ ATPase (PfSERCA) Gene Diversity

Author

Jambou, Ronan, primary, Martinelli, Axel, additional, Pinto, João, additional, Gribaldo, Simonetta, additional, Legrand, Eric, additional, Niang, Makhtar, additional, Kim, Nimol, additional, Pharath, Lim, additional, Volnay, Béatrice, additional, Ekala, Marie Therese, additional, Bouchier, Christiane, additional, Fandeur, Thierry, additional, Berzosa, Pedro, additional, Benito, Agustin, additional, Ferreira, Isabel Dinis, additional, Ferreira, Cynthia, additional, Vieira, Pedro Paulo, additional, Alecrim, Maria das Graças, additional, Mercereau-Puijalon, Odile, additional, and Cravo, Pedro, additional

Published
2010
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