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Die Samen von Digitalis purpurea L. enthalten ein stark ungesattigtes Ol, mit Olsaure, Linolsaure und Linolensaure als Bestandteile der flussigen Fettsauren. Die Analysenresultate sowie die angewandten Untersuchungsmethoden sind wiedergegeben. The Seed-Oil of Digitalis purpurea L. Seeds of digitalis purpurea L. contain a highly unsaturated oil with oleic acid, linoleic acid and linolenic acid as components of the liquid fatty acids. Analytical values are given and the methods of analysis described. more...

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The sugar and chlorophyll content of alcoholic extracts of oven-dried and lyophilized leaves of Digitalis purpurea and Digitalis lutea are reported. Freeze-drying preserves more total chlorophyll and chlorophyll “a” than oven-drying. Little difference in chlorophyll “b” was observed with material dried by either method. Oven-drying increases the-amount of sugars extracted with alcohol U. S. P. from Digitalis lutea. Freeze-drying does not have any more effect than oven-drying on the amount of carbohydrate material extracted with alcohol U. S. P. from Digitalis purpurea. more...

Une intoxication chez un homme de 70 ans, apres absorption d'une infusion d'herbes. Des feuilles de digitale ont ete confondues avec celles de Symphytum

A S MANY of you are aware, digitalis consists of an extract of the leaves of the plant Digitalis purpurea. In this respect it is a comparatively simple biological preparation. Chemically, however, it is exceedingly complex and its exact nature is not yet elucidated. It is known, however, that there are present more than 25 glycosides, some of which, like digitoxin, have been prepared in a pure chemical form, others of which are of incompletely proven constitution. Moreover, two samples of leaf may contain widely different proportions of these 25 or more glycosides and in some samples a few of them may be altogether absent. It is also known that there are often large variations in the potency ratio of two samples of digitalis when the test animal is changed, or when the conditions of the assay in any one species of test animal are changed. In the first case this can be attributed to a varying sensitivity of the animal species to different glycosides which are present in the two samples in different proportions and, in the second place, to quote one example, the different potency ratios obtained in the past between frog assays read at 2 hours and at 24 hours could be attributed to different proportions of a slowly-acting glycoside which produced its effects only after the end of the 2-hour period. In addition to these facts, neither the cat nor the frog is human, and neither the guinea-pig nor the pigeon suffers as far as I am aware from auricular fibrillation. These, then, are the major difficulties and they are indeed formidable. In fact, the assay of such heterogeneous material must always be regarded as a makeshift-as a practical necessity in lieu of something better-and we must always beware of attaching too great a degree of precision or of definition to the final estimate of potency. When it became necessary to establish a Third International Digitalis Standard some time ago an attempt was made to minimise *The full report of the results and analysis of the collaborative assay, a summary of which was circulated to members of the Conference, wvill be published elsewhere. more...

The following anthraquinones have been found in the leaves of D. purpurea; digitolutein and 4-hydroxydigitolutein, digitopurpone (1,4,8-trihydroxy-2-methylanthraquinone), 1,6-dihydroxy-3-methylanthraquinone (phomarin) and its 6-methyl ether, and isochrysophanol (1,8-dihydroxy-2-methylanthraquinone). more...

Excerpt There is no member of the pharmacopeia more fascinating and intriguing than digitalis. The history of this drug is almost a medical epic. Its botanical name, Digitalis purpurea, was given t... more...

SEVERAL of the constituents of Digitalis purpurea have been separated by paper partition chromatography, using ethylene glycol as the stationary phase and chloroform saturated with ethylene glycol as the mobile phase. more...

HYBRIDS have often been obtained between Digitalis purpurea and D. ambigua, but it was not until 1926 that a few F2 seedlings were raised by crossing such hybrids inter se.1

Background Digitalis purpurea is an important ornamental and medicinal plant. There is considerable interest in exploring its transcriptome. Results Through high-throughput 454 sequencing and subsequent assembly, we obtained 23532 genes, of which 15626 encode conserved proteins. We determined 140 unigenes to be candidates involved in cardiac glycoside biosynthesis. It could be grouped into 30 families, of which 29 were identified for the first time in D. purpurea. We identified 2660 mRNA-like npcRNA (mlncRNA) candidates, an emerging class of regulators, using a computational mlncRNA identification pipeline and 13 microRNA-producing unigenes based on sequence conservation and hairpin structure-forming capability. Twenty five protein-coding unigenes were predicted to be targets of these microRNAs. Among the mlncRNA candidates, only 320 could be grouped into 140 families with at least two members in a family. The majority of D. purpurea mlncRNAs were species-specific and many of them showed tissue-specific expression and responded to cold and dehydration stresses. We identified 417 protein-coding genes with regions significantly homologous or complementary to 375 mlncRNAs. It includes five genes involved in secondary metabolism. A positive correlation was found in gene expression between protein-coding genes and the homologous mlncRNAs in response to cold and dehydration stresses, while the correlation was negative when protein-coding genes and mlncRNAs were complementary to each other. Conclusions Through comprehensive transcriptome analysis, we not only identified 29 novel gene families potentially involved in the biosynthesis of cardiac glycosides but also characterized a large number of mlncRNAs. Our results suggest the importance of mlncRNAs in secondary metabolism and stress response in D. purpurea. more...