351. Effect of interaction of magnetic nanoparticles of Fe₃O₄ and artesunate on apoptosis of K562 cells.
- Author
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Wang Y, Han Y, Yang Y, Yang J, Guo X, Zhang J, Pan L, Xia G, and Chen B
- Subjects
- Analysis of Variance, Artemisinins chemistry, Artesunate, Blotting, Western, Caspase 3 metabolism, Dose-Response Relationship, Drug, Down-Regulation drug effects, Drug Interactions, Flow Cytometry, Humans, Inhibitor of Apoptosis Proteins metabolism, K562 Cells, Magnetite Nanoparticles chemistry, Proto-Oncogene Proteins c-bcl-2 metabolism, Survivin, Apoptosis drug effects, Artemisinins pharmacology, Magnetite Nanoparticles administration & dosage
- Abstract
The present study evaluated whether the magnetic nanoparticles of Fe(3)O(4) (MNPs-Fe(3)O(4)) could enhance the activity of artesunate (ART), and to explore its potential mechanisms. Cytotoxicity of the copolymer of ART with MNPs-Fe(3)O(4) on K562 cells was detected by MTT assay and the apoptosis rate of K562 cells was measured by flow cytometry. Protein expression levels of bcl-2, bax, bcl-rambo, caspase-3, and survivin in K562 cells were measured by Western blot. After being incubated with the copolymer of ART with MNPs-Fe(3)O(4) for 48 hours, the growth inhibition rate of K562 cells was significantly increased compared with that of K562 cells treated with ART alone (P < 0.05), and the apoptosis rate of K562 cells was increased significantly compared with that of K562 cells treated with ART alone, suggesting that MNPs-Fe(3)O(4) can enhance the activity of ART. Interestingly, the copolymer-induced cell death was attenuated by caspase inhibitor Z-VAD-FMK. Our results also showed that treatment with the copolymer of MNPs-Fe(3)O(4) and ART increased the expression of bcl-2, bax, bcl-rambo, and caspase-3 proteins, and decreased the expression of survivin protein in K562 cells compared with ART treatment alone. These results suggest that MNPs-Fe(3)O(4) can enhance ART-induced apoptosis, which may be related to the upregulation of bcl-rambo and downregulation of survivin.
- Published
- 2011
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