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Phalsa is an important berry fruit in Asia, but little is known of its postharvest characteristics and especially changes in fruit composition during storage. Investigations were made for nutritional and antioxidative enzymatic changes in the juice extracted from two different maturity stages (M 1 : pink to red or M 2 : dark red to purple) during low temperature storage for 0, 2, 4, 6, 8 and 10 days at 5 ± 1 °C and 65 ± 5% RH. The fruit showed significant gradual declines in ascorbic acid (Vitamin C), total phenolics (TPC), total antioxidant capacity (TAC), total carotenoids, anthocyanins, moisture%, crude protein, crude fiber and fat content and the activity of superoxide dismutase (SOD) during storage. However, the total soluble solids (TSS), pH, ash content, and the activity of catalase (CAT) and peroxidase (POD) increased significantly. The polynomial regression analysis indicated that these changes were either quadratic or linear; and the Pearson's correlation among the studied parameters was significant. More mature fruit had higher concentrations of TSS, ascorbic acid, TPC, TAC, total carotenoids and anthocyanins, more juice and less weight-loss compared with less mature fruit. Overall, significant nutritive functions and antioxidative enzymes were found in both maturity stages of phalsa fruit, which experienced significant changes during postharvest storage. • The juice of ripe phalsa fruit has better nutritional properties than early mature. • Various biochemicals, bioactive compounds and natural pigments in phalsa juice significantly reduce under storage. • The proximate composition of phalsa fruit juice is influenced by fruit harvest maturity and low temperature storage. • POD enzyme activity in phalsa fruit juice increases while SOD and CAT decline during storage. [ABSTRACT FROM AUTHOR]

Abstract: In any finish milling process the minimization of surface roughness is the prime objective, and if the workpiece belongs to the category of ultra-hard steels then the objective of maximizing tool life also gains considerable importance. In this research work, the effects of four parameters, namely, hardened steel''s microstructure, workpiece inclination angle, cutting speed, and radial depth of cut were studied upon tool life and surface roughness (in directions of feed and pick-feed). The milling was performed under environment of minimum quantity of lubrication (MQL), using coated carbide ball-nose end mills. The quantification of the aforementioned effects was done using a new response surface methodology known as the D-optimal method. For tool life, workpiece material was found as the most influential parameter followed by the rotational speed of tool. High values of tool''s rotational speed proved unfavorable for tool life but favorable for surface finish. In addition, the effects of workpiece inclination angle and radial depth of cut were analyzed upon effective cutting speed and cusp height and, subsequently, upon surface roughness. SEM and EDS analyses of the worn-out tools were carried out in order to study the effects of different levels of parameters selected upon the severity of different tool damage modes. The major tool damage mechanisms detected were notch wear, adhesion, and chipping. The severity of chipping was relatively smaller as compared to that of adhesion and of notch wear because of reduced effective cutting speeds and feed rate employed. [Copyright &y& Elsevier]

Abstract: The main focus of research in hard-milling domain has been the enhancement of tool life and the improvement in workpiece surface quality. This paper deals with the application of expert system technology in order to use the experimental data for optimization of milling parameters so as to achieve targets of enhancing tool life and improving workpiece surface finish. Hard-milling experiments were conducted to study the effects of workpiece material hardness, cutter’s helix angle, milling orientation and coolant upon tool life, workpiece surface roughness, and cutting forces. The experimental data were converted to useful information using ANOVA and numeric optimization, and this information was used to develop the knowledge-base in form of IF–THEN rules. Expert system utilized fuzzy logic for its reasoning mechanism, while, fuzzy data sets and crisp sets were freely mixed in antecedents and consequents of the rules. Effectiveness of the expert system was based upon two modules, namely optimization module and prediction module, with each of them operating upon different set of rules. Optimization module provides the optimal selection and combination of aforementioned milling parameters according to the desired objective, while the prediction module provides the prediction of performance measures for the combination of parameters finalized by the optimization module. [Copyright &y& Elsevier]

Abstract Background Aedes aegypti mosquitoes are the main vectors of dengue viruses to humans. Understanding their biology and interactions with the pathogen are prerequisites for development of dengue transmission control strategies. Mosquito salivary glands are organs involved directly in pathogen transmission to vertebrate hosts. Information on the spatial distribution of gene expression in these organs is expected to assist in the development of novel disease control strategies, including those that entail the release of transgenic mosquitoes with impaired vector competence. Results We report here the hybridization in situ patterns of 30 transcripts expressed in the salivary glands of adult Ae. aegypti females. Distinct spatial accumulation patterns were identified. The products of twelve genes are localized exclusively in the proximal-lateral lobes. Among these, three accumulate preferentially in the most anterior portion of the proximal-lateral lobe. This pattern revealed a salivary gland cell type previously undescribed in Ae. aegypti, which was validated by transmission electron microscopy. Five distinct gene products accumulate in the distal-lateral lobes and another five localize in the medial lobe. Seven transcripts are found in the distal-lateral and medial lobes. The transcriptional product of one gene accumulates in proximal- and distal-lateral lobes. Seven genes analyzed by quantitative PCR are expressed constitutively. The most abundant salivary gland transcripts are those localized within the proximal-lateral lobes, while previous work has shown that the distal-lateral lobes are the most active in protein synthesis. This incongruity suggests a role for translational regulation in mosquito saliva production. Conclusions Transgenic mosquitoes with reduced vector competence have been proposed as tools for the control of dengue virus transmission. Expression of anti-dengue effector molecules in the distal-lateral lobes of Ae. aegypti salivary glands has been shown to reduce prevalence and mean intensities of viral infection. We anticipate greater efficiency of viral suppression if effector genes are expressed in all lobes of the salivary glands. Based on our data, a minimum of two promoters is necessary to drive the expression of one or more anti-dengue genes in all cells of the female salivary glands.

Developing an efficient, robust, and noble metal-free electrocatalyst that can catalyse oxygen evolution reactions (OER) remains a significant challenge. CoS 2 , a representative of pyrite form transition metal dichalcogenides, has recently been identified as an economical catalyst. Here, an incredibly quick and scalable technique for novel catalysts synthesized with the use of the microwave method was introduced. Manganese-doped cobalt sulphide (Mn-CoS 2 ) showed outstanding OER with a very low overpotential of 227 mV at 10 mA cm -2 . Exposure of surface atoms resulted in high electrochemical activity, where the defects facilitated charge and mass transfer along the nanostructure, allowing surface dependent electrochemical reactions to be performed more efficiently. The electronic properties of pristine and transition-metal-doped CoS 2 structures were also investigated using density functional theory (DFT). To better understand transition metal's dependent impact on crystal structure, orbital electronic participation, charge density, and charge transformation in both pristine and Mn-dopedCoS 2 frameworks were calculated and analysized. Our synthesis approach is primarily commercial and extensible, overcoming synthesis challenge of transition metal sulphide nanostructures with prime quality and implying a potential for commercial uses., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Background: Mosquito Control Programs are articulated to control Mosquito Borne Diseases and success of such programs depends on the activities of field workers, and their adherence to the standard operating procedures (SOP's) is governed by their knowledge, attitudes, and practices (KAP). Present study was intended to assess the KAP of mosquito control workers of Pakistan to get an exact depiction of prevailing situation., Methods: A cross-sectional descriptive study was conducted in March-April 2020. Questionnaire containing 30 closed and open-ended questions were administrated to participants. Knowledge and practices were evaluated using a scoring system i.e., by giving 1 point to each correct answer while attitude questions were analyzed individually and expressed in percentage for each response., Results: Total 639 workers were interviewed, mean age was 29.8 (SD ±7.87) years, majority (65.1%) was in age group of 18-30 years. Mean knowledge score was 6.96±1.28 (range 0-9) with 77.36% correct answers (P= 0.073). Mean practices score was 7.00±1.62 (range 2-9) with 77.83% appropriate answers (P< 0.001). Both knowledge and practices scores were higher for permanent employees, practices score increased with increase in job experience. Very weak positive correlation (r= 0.127) was observed between knowledge and practice scores., Conclusion: Appropriate practice correlates with better knowledge and positive attitude towards control activities. Hence, training on protection and protective measures for having a positive attitude among healthcare workers is necessary against the fight with mosquitoes., Competing Interests: Conflict of interest statement The authors declare there is no conflict of interests., (Copyright © 2023 The Authors. Published by Tehran University of Medical Sciences.)

Background: Camel is vital to daily life as a source of food and means of transportation, and just as importantly, its milk and urine have been used as medicine for diverse ailments since ancient times for people living in the desert region., Aim: The current study was planned to investigate the effect of different watering regimes on physiological and hematological parameters of lactating Marecha camels during the summer season., Methods: For this purpose, 12 lactating she-camels of almost similar age (8-13 years) and parity (2-5) were selected in a completely randomized design with four animals per treatment at the Camel Breeding and Research Station Rakh Mahni District Bhakkar. The trial was completed in 60 days, while 7 days were given as an acclimatization period for adaptation. The treatments were as follows: Group-1 (G-1) considered as the control had access to water once in a day; Group-2 (G-2) had access to water once in 4 days, and Group-3 (G-3) had access to water once in 6 days. Animals were housed individually for feeding and watering in an intensive management system. The same feed consisting of green fodder ( Lucerne ) and gram straw ( Cicer arientinum ) was offered to all animals. The average ambient temperature and relative humidity were 39°C-41°C and 55%-63%, respectively., Result: The mean values ( p < 0.001) of water intake were found to be 82.94 ± 1.34 l in G-3, which was higher than G-1 and G-2. Blood analysis showed that packed cell volume, white blood cells, and hemoglobin were affected significantly ( p < 0.001, p < 0.01, and p < 0.05, respectively)., Conclusion: No serious changes in complete blood count and physiological parameters were reported in this study. So, this shows that the camel is unique in its physiological adaptation in hot and hostile environments., Competing Interests: The authors declare that there is no conflict of interest.

Healthcare workers are at the highest risk of contracting novel coronavirus disease 2019 (COVID-19) and, therefore, require constant protection. This study assesses access to personal protective equipment (PPE), availability of adequate information about PPE use, self-reported ability to correctly wear and remove (donning and doffing) PPE, and risk perceptions associated with COVID-19 among frontline healthcare workers in Pakistan. Using a structured and validated questionnaire, an online survey was conducted from May 9 to June 5, 2020. Responses were received from 453 healthcare workers. Of these, 218 (48.12%) were doctors, 183 (40.40%) were nurses, and 52 (11.48%) were paramedical staff. Only 129 (28.48%) healthcare workers reported having adequate access to PPE at all times, whereas 156 (34.44%) never had access to PPE and 168 (37.09%) had access to PPE occasionally. Lack of access to PPE led the majority (71.74%) of healthcare workers to use coping strategies such as reuse of N95 and surgical masks. A total of 312 (68.87%) respondents believed that the risk of contracting COVID-19 in the work environment was high and the majority (62.69%) adopted precautionary measures at home to keep their families safe. A significantly high (n = 233, 51.43%, P  = .03) number of respondents reported self-medicating. Of all the respondents, only 136 (30.02%) were tested for COVID-19 at least once, of which 32 (23.53%) ever tested positive. These findings suggest that healthcare workers in Pakistan had limited access to PPE. Adequate provision and training is vital to protect the healthcare workforce during the COVID-19 pandemic.

In developed and underdeveloped countries, ticks are important vectors transmitting various pathogens that cause diseases of veterinary and public health importance, like babesiosis, theileriosis, Crimean‑Congo haemorrhagic fever (CCHF) and many more. Many species of ticks have been reported in scientific literature from Pakistan, which need to be listed for ready reference. For this purpose, a checklist of tick species recorded in Pakistan is presented here after comprehensive review of the available literature on the subject. Overall, nine genera and 53 species of ticks infesting animals in Pakistan are presented in this checklist.

Plant-derived natural products are of great interest due to their diversity in modern drug discovery. Sarcococca saligna has been used for the treatment of different diseases. The present study was aimed at isolating phytochemical constituents including Alkaloid-C (a), Dictyophlebine (b), Sarcovagine-D (c) and Saracodine (d) Holaphylline (e) from Sarcococca saligna to investigate the anticancer effect of these compounds. These compounds were evaluated for inhibition of aromatase enzyme of breast cancer in assistance by molecular docking simulations to understand molecular interaction between the enzyme and ligands. The IC 50 values of compound 1 and 5 were found 138.27 ± 0.01 µl and 12.91 ± 0.01 µl, respectively, and both were found active due to their bulky structures in comparison to the active site of aromatase enzyme. The standard drug exemestane showed potent activity in comparison with the test compounds, having IC 50 values of 0.052 ± 0.01 µl. Both compounds showed favorable electrostatic interactions with the active site of aromatase enzyme but the shape and steric bulk of the compounds was the limiting factor in their inhibitory effects. New lead compounds could be generated after extensive modifications guided by computational and experimental tools as a possible anticancer agents by targeting aromatase enzyme., (© 2019 John Wiley & Sons A/S.)

Indian siris, Albizia lebbeck (L.) Benth. (Fabaceae: Mimosoideae) has significant importance to human beings for its multipurpose use. Insects play a crucial role in the pollination biology of flowering plants. In the current study, we studied the pollination biology of A. lebbeck with special reference to insect floral visitors. The effectiveness of floral visitors was investigated in term of visitation frequency, visitation rate and pollen load during 2012 and 2013. In the second experiment, effect of pollinators on yield of A. lebbeck was studied in open and cage pollination experiments. Floral visitor fauna of A. lebbeck included eight-bees, two wasps, two flies, and two butterflies species. Among them, Apis dorsata , Apis florea , Amegilla cingulata, and Nomia oxybeloides had maximum abundance ranging from 349-492, 339-428, 291-342 and 235-255 numbers of individuals, respectively during two flowering seasons. A. dorsata had the highest visitation frequency (6.44 ± 0.49-8.78 ± 0.48 visits/flower/5min) followed by Amegilla cingulata (6.03 ± 0.43-7.99 ± 0.33 visits/flower/5min) and A. florea (3.61 ± 0.31-4.44 ± 0.18 visits/flower/5min). A. dorsata , N. oxybeloides, and Amegilla cingulata had the highest visitation rates (18.904 ± 1.53-11.43 ± 1.17 flower visited/min) and pollen load (15333 ± 336.22-19243 ± 648.45 pollen grains). The open pollinated flowers had significantly higher capsule weight (4.97 ± 0.21 g), seed weight (1.04 ± 0.05 g), seed numbers per pod (9.80 ± 0.34) and seed germination percentage (84.0 ± 1.78%) as compared to caged flowers. The results suggested bees especially A. dorsata , N. oxybeloides and Amegilla cingulata could be effective pollinators of A. lebbeck ., (© 2018 Production and hosting by Elsevier B.V. on behalf of King Saud University.)

Objective: To evaluate the psychological well-being of substance use patients in comparison of combined religious therapy and pharmacotherapy effects with that of pharmacotherapy effects alone and also to assess the psychological well-being of drug addicts in comparison of demographics characteristics., Methods: This experimental study was conducted at Department of Psychology, University of Gujrat from July 5 th 2016 to July 25 th 2017. A sample of 140 drug addicted patients was taken from different hospital. The pre and post-test of experimental and control group was done. Experimental group received standard pharmacotherapy along with religious therapy while patients in the control group only induced standard pharmacotherapy. The effectiveness of therapy was judged on their psychological well-being using California Psychological Inventory Well-being sub-scale.., Results: The results of the study confirmed that there was significant difference in the psychological well-being of control and experimental group (p-value <0.01). After the treatment a difference exists in the means of control and experimental (16.24 and 26.44 respectively) groups. An increase in the psychological well-being of those having religious therapy comparing to those not having religious therapy was observed. Further, comparing the demographic variables the means indicated that treatment affected all age groups, marital status and education level equally. Whereas, in the rural area (mean, 27.04) psychological well-being was better than the urban (mean=26.11) and with the income levels of 21,000 to 30,000 (mean, 27.57) there was more improvement as compared with other income levels (mean, 26.35 and mean, 26.03)., Conclusion: The religious therapy had a significant therapeutic effect on psychological well-being of the substance use patients and it is equally effective for all age group, marital status and educational level.

From the Field is a semi-regular column that explores what it means to be a local health professional on the front lines of an emergency. Typically, National Association of County and City Health Officials (NACCHO) members share their stories of preparing for and responding to disasters, epidemics, and other major health issues. Through exploring the analysis of the challenges faced and the solutions developed, readers can learn how these public health champions keep their communities safe even in extreme situations. Readers may submit topics of interest to the column's editor, Meghan McGinty, PhD, MPH, MBA, at mmcginty@naccho.org.

Lithium recovery from an aqueous resource was accelerated by electrochemically driving the transformation of Mn IV /Mn III with a spinel λ-MnO 2 film electrode. A λ-MnO 2 electrode without binders or conductive additives is preferred for achieving a large capacity at high current density and long-term cycling capability. In this study, a film of Mn(OH) 2 was first deposited on the surface of Pt or graphite substrates owing to alkalization near the cathode, then it was oxidized to a Mn 3 O 4 film by air, followed by being hydrothermally lithiated to LiMn 2 O 4 spinel and, finally, it was turned into the λ-MnO 2 film electrode through potentiostatic delithiation. The results show that the charging/discharging electric capacity of the fabricated λ-MnO 2 film electrode was up to ≈100 mAh g -1 at a current density of 50 mA g -1 in 30 mm Li + aqueous solution, twice that of the λ-MnO 2 powder electrode. Also, 82.3 % lithium capacity remained after 100 cycles of an electrochemically assisted lithium recovery process, indicating high availability and good stability of the λ-MnO 2 spinel on the electrode. The energy consumption for each cycle is estimated to be approximately 1.55±0.09 J, implying that only 4.14 Wh is required for recovery of one mole of lithium ions by this method., (© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)

A 66-year-old Caucasian female was referred to the ear, nose and throat outpatient by her general practitioner with complaints of worsening sore throat, hoarseness of voice and productive cough for 3 months. The patient also had a history of rigors and evening temperature. She was using long-term steroids for nephrotic syndrome. The patient was treated for pulmonary tuberculosis as a child. An exophytic left supraglottic mass involving the left aryepiglottic fold, epiglottis and left vocal cord was revealed on fibre-optic laryngoscopy. The diagnosis of laryngeal tuberculosis was confirmed on repeat biopsy. The sputum smear was also positive for acid-fast bacilli. Chest x ray reported fibrosis of the right upper lobe indicating tuberculosis. The patient was started on anti-tuberculous treatment and made a steady recovery. There is reactivation of pulmonary tuberculosis in our case report with secondary involvement of larynx probably due to long-term use of steroids for nephrotic syndrome.

Objectives: To translate The World Health Organization quality of life scale, WHO-QOL BREF, in Urdu and validate it., Design: A descriptive study., Place and Duration of Study: Department of Psychiatry, Postgraduate Medical Institute, Lahore, from July 2002 to October 2002., Materials and Methods: The English version of WHO-QOL BREF was translated in Urdu and later back translated. The process was checked and evaluated at each step by a translation committee comprising of group of bilingual experts. The whole process was carried out in four stages. The translated version was further evaluated statistically in three ways to check linguistic equivalence, concept equivalence and scale equivalence., Results: A significant level of equivalence was seen at all parameters., Conclusion: This study concludes that the Urdu version of WHO-QOL BREF is a reliable and valid version to be used in our population to measure the quality of life in Pakistan.