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Automatic quantification of cell nuclei in immunostained images is highly desired by pathologists in diagnosis. In this paper, we present a new approach for the segmentation of severely clustered overlapping nuclei. The proposed approach first involves applying a combined global and local threshold method to extract foreground regions. In order to segment clustered overlapping nuclei in the foreground regions, seed markers are obtained by utilizing morphological filtering and intensity based region growing. Seeded watershed is then applied and clustered nuclei are separated. As pixels corresponding to stained cellular cytoplasm can be falsely identified as belonging to nuclei, a post processing step identifying positive nuclei pixels is added to eliminate these false pixels. This new approach has been tested on a set of manually labeled Tissue Microarray (TMA) and Whole Slide Images (WSI) colorectal cancers stained for the biomarker P53. Experimental results show that it outperformed currently available state of the art methods in nuclei segmentation.

We have developed a new face hallucination framework termed from local pixel structure to global image super-resolution (LPS-GIS). Based on the assumption that two similar face images should have similar local pixel structures, the new framework first uses the input low-resolution (LR) face image to search a face database for similar example high-resolution (HR) faces in order to learn the local pixel structures for the target HR face. It then uses the input LR face and the learned pixel structures as priors to estimate the target HR face. We present a three-step implementation procedure for the framework. Step 1 searches the database for K example faces that are the most similar to the input, and then warps the K example images to the input using optical flow. Step 2 uses the warped HR version of the K example faces to learn the local pixel structures for the target HR face. An effective method for learning local pixel structures from an individual face, and an adaptive procedure for fusing the local pixel structures of different example faces to reduce the influence of warping errors, have been developed. Step 3 estimates the target HR face by solving a constrained optimization problem by means of an iterative procedure. Experimental results show that our new method can provide good performances for face hallucination, both in terms of reconstruction error and visual quality; and that it is competitive with existing state-of-the-art methods.

Image patches of different spatial frequencies are likely to have different perceptual significance as well as reflect different physical properties. Incorporating such concept is helpful to the development of more effective image retrieval techniques. We introduce a method which separates an image into layers, each of which retains only pixels in areas with similar spatial frequency characteristics and uses simple low-level features to index the layers individually. The scheme associates indexing features with perceptual and physical significance thus implicitly incorporating high level knowledge into low level features. We present a computationally efficient implementation of the method, which enhances the power and at the same time retains the simplicity and elegance of basic color indexing. Experimental results are presented to demonstrate the effectiveness of the method.

This paper presents a new application of a well-studied image coding technique, namely block truncation coding (BTC). It is shown that BTC can not only be used for compressing color images, it can also be conveniently used for content-based image retrieval from image databases. From the BTC compressed stream (without performing decoding), we derive two image content description features, one termed the block color co-occurrence matrix (BCCM) and the other block pattern histogram (BPH). We use BCCM and BPH to compute the similarity measures of images for content-based image retrieval applications. Experimental results are presented which demonstrate that BCCM and BPH are comparable to similar state of the art techniques.