572 results on '"Sokolov, Vladimir"'
Search Results
552. Synthesis of 3-fluoro-2-(diethoxyphosphoryl)imidazo[1,2-a]pyridine
- Author
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Aksinenko, Alexei Yu., Goreva, Tatyana V., Epishina, Tatyana A., and Sokolov, Vladimir B.
- Subjects
- *
PHOSPHORYL group , *IMIDAZOLES , *ORGANIC synthesis , *PYRIDINE , *CHEMICAL reactions , *PHOSPHITES - Abstract
Abstract: The first representative of 3-fluoro-2-(dialkoxyphosphoryl)imidazo[1,2-a]pyridines has been synthesized by the reaction of N-(pyridin-2-yl)-2,2,2-trifluoroacetimidoyl chloride with triethyl phosphite. [Copyright &y& Elsevier]
- Published
- 2012
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553. New Drosophila promoter-associated architectural protein Mzfp1 interacts with CP190 and is required for housekeeping gene expression and insulator activity.
- Author
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Sokolov V, Kyrchanova O, Klimenko N, Fedotova A, Ibragimov A, Maksimenko O, and Georgiev P
- Subjects
- Animals, Heterochromatin metabolism, Heterochromatin genetics, Genes, Essential, Transcription Factors metabolism, Transcription Factors genetics, Protein Binding, Gene Expression Regulation, Euchromatin metabolism, Euchromatin genetics, DNA-Binding Proteins metabolism, DNA-Binding Proteins genetics, Microtubule-Associated Proteins, Drosophila Proteins metabolism, Drosophila Proteins genetics, Promoter Regions, Genetic, Insulator Elements genetics, Drosophila melanogaster genetics, Drosophila melanogaster metabolism, Nuclear Proteins metabolism, Nuclear Proteins genetics
- Abstract
In Drosophila, a group of zinc finger architectural proteins recruits the CP190 protein to the chromatin, an interaction that is essential for the functional activity of promoters and insulators. In this study, we describe a new architectural C2H2 protein called Madf and Zinc-Finger Protein 1 (Mzfp1) that interacts with CP190. Mzfp1 has an unusual structure that includes six C2H2 domains organized in a C-terminal cluster and two tandem MADF domains. Mzfp1 predominantly binds to housekeeping gene promoters located in both euchromatin and heterochromatin genome regions. In vivo mutagenesis studies showed that Mzfp1 is an essential protein, and both MADF domains and the CP190 interaction region are required for its functional activity. The C2H2 cluster is sufficient for the specific binding of Mzfp1 to regulatory elements, while the second MADF domain is required for Mzfp1 recruitment to heterochromatin. Mzfp1 binds to the proximal part of the Fub boundary that separates regulatory domains of the Ubx and abd-A genes in the Bithorax complex. Mzfp1 participates in Fub functions in cooperation with the architectural proteins Pita and Su(Hw). Thus, Mzfp1 is a new architectural C2H2 protein involved in the organization of active promoters and insulators in Drosophila., (© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.)
- Published
- 2024
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554. Transcriptional Readthrough Interrupts Boundary Function in Drosophila.
- Author
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Kyrchanova O, Sokolov V, Tikhonov M, Manukyan G, Schedl P, and Georgiev P
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- Animals, Drosophila genetics, Drosophila metabolism, Homeodomain Proteins genetics, Genes, Insect, Drosophila melanogaster genetics, Drosophila melanogaster metabolism, Drosophila Proteins genetics, Drosophila Proteins metabolism, RNA, Long Noncoding
- Abstract
In higher eukaryotes, distance enhancer-promoter interactions are organized by topologically associated domains, tethering elements, and chromatin insulators/boundaries. While insulators/boundaries play a central role in chromosome organization, the mechanisms regulating their functions are largely unknown. In the studies reported here, we have taken advantage of the well-characterized Drosophila bithorax complex (BX-C) to study one potential mechanism for controlling boundary function. The regulatory domains of BX-C are flanked by boundaries, which block crosstalk with their neighboring domains and also support long-distance interactions between the regulatory domains and their target gene. As many lncRNAs have been found in BX-C, we asked whether readthrough transcription (RT) can impact boundary function. For this purpose, we took advantage of two BX-C boundary replacement platforms, Fab-7
attP50 and F2attP , in which the Fab-7 and Fub boundaries, respectively, are deleted and replaced with an attP site. We introduced boundary elements, promoters, and polyadenylation signals arranged in different combinations and then assayed for boundary function. Our results show that RT can interfere with boundary activity. Since lncRNAs represent a significant fraction of Pol II transcripts in multicellular eukaryotes, it is therefore possible that RT may be a widely used mechanism to alter boundary function and regulation of gene expression.- Published
- 2023
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555. Transcriptional read through interrupts boundary function in Drosophila.
- Author
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Kyrchanova O, Sokolov V, Tikhonov M, Schedl P, and Georgiev P
- Abstract
In higher eukaryotes enhancer-promoter interactions are known to be restricted by the chromatin insulators/boundaries that delimit topologically associated domains (TADs); however, there are instances in which enhancer-promoter interactions span one or more boundary elements/TADs. At present, the mechanisms that enable cross-TAD regulatory interaction are not known. In the studies reported here we have taken advantage of the well characterized Drosophila Bithorax complex (BX-C) to study one potential mechanism for controlling boundary function and TAD organization. The regulatory domains of BX-C are flanked by boundaries which function to block crosstalk with their neighboring domains and also to support long distance interactions between the regulatory domains and their target gene. As many lncRNAs have been found in BX-C, we asked whether transcriptional readthrough can impact boundary function. For this purpose, we took advantage of two BX-C boundary replacement platforms, Fab-7
attP50 and F2attP , in which the Fab-7 and Fub boundaries, respectively, are deleted and replaced with an attP site. We introduced boundary elements, promoters and polyadenylation signals arranged in different combinations and then assayed for boundary function. Our results show that transcriptional readthrough can interfere with boundary activity. Since lncRNAs represent a significant fraction of Pol II transcripts in multicellular eukaryotes, it is possible that many of them may function in the regulation of TAD organization., Author Summary: Recent studies have shown that much genome in higher eukaryotes is transcribed into non-protein coding lncRNAs. It is though that lncRNAs may preform important regulatory functions, including the formation of protein complexes, organization of functional interactions between enhancers and promoters and the maintenance of open chromatin. Here we examined how transcription from promoters inserted into the Drosophila Bithorax complex can impact the boundaries that are responsible for establishing independent regulatory domains. Surprisingly, we found that even a relatively low level of transcriptional readthrough can impair boundary function. Transcription also affects the activity of enhancers located in BX-C regulatory domains. Taken together, our results raise the possibility that transcriptional readthrough may be a widely used mechanism to alter chromosome structure and regulate gene expression.- Published
- 2023
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556. Mechanisms of Interaction between Enhancers and Promoters in Three Drosophila Model Systems.
- Author
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Kyrchanova O, Sokolov V, and Georgiev P
- Subjects
- Animals, Promoter Regions, Genetic, Drosophila melanogaster genetics, Mammals metabolism, Drosophila genetics, Drosophila metabolism, Drosophila Proteins metabolism
- Abstract
In higher eukaryotes, the regulation of developmental gene expression is determined by enhancers, which are often located at a large distance from the promoters they regulate. Therefore, the architecture of chromosomes and the mechanisms that determine the functional interaction between enhancers and promoters are of decisive importance in the development of organisms. Mammals and the model animal Drosophila have homologous key architectural proteins and similar mechanisms in the organization of chromosome architecture. This review describes the current progress in understanding the mechanisms of the formation and regulation of long-range interactions between enhancers and promoters at three well-studied key regulatory loci in Drosophila.
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- 2023
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557. Reactivity of Transition Metal Gallylene Complexes Toward Substrates with Multiple Carbon-Element Bonds.
- Author
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Dodonov VA, Sokolov VG, Baranov EV, Skatova AA, Xu W, Zhao Y, Yang XJ, and Fedushkin IL
- Abstract
Reactivity of transition metal complexes containing the redox-active gallylene (dpp-bian)Ga ligand (dpp-bian = 1,2-bis[(2,6-diisopropylphenyl)imino]acenaphthene) toward isocyanide, isocyanate, isothiocyanate, and ketene substrates is described. The reaction of [(dpp-bian)GaCr(CO)
5 ] ( 1 ) with tBuNC results in a dative complex [(dpp-bian)Ga(CNtBu)Cr(CO)5 ] ( 2 ), while compound [(dpp-bian)GaCr(CO)5 ]2 [Na(THF)2 ]2 ( 3 ) reacts with tBuNC to give the coordination polymer [(dpp-bian)GaCr(CO)5 ][Na(CNtBu)(THF)]n ( 5 ). Treatment of [(dpp-bian)GaCr(CO)5 ]2 [Na(THF)2 ]2 with an excess of PhNCO results in trimerization of the latter and formation of complex [(dpp-bian)GaCr(CO)5 ][Na(PhNCO)3 (Et2 O) (DME)] ( 4 ). [(dpp-bian)GaFeCp(CO)2 ] ( 7 ) treated with Ph2 CCO or PhNCS results in cycloaddition products [(dpp-bian)(Ph2 CCO)GaFeCp(CO)2 ] ( 8 ) and [(dpp-bian)(PhNCS)GaFeCp(CO)2 ] ( 9 ). The formation of 2 and 9 was found to be reversible, which offers a means for facile regulation of transition metal center reactivity and cooperative substrate activation. New compounds were characterized by EPR ( 2 ), NMR ( 4 , 8 , and 9 ), and IR spectroscopy ( 2 , 4 , 5 , 8 , and 9 ). The molecular structures of 2 , 4 , 5 , 8 , and 9 were established by single-crystal X-ray diffraction analysis. Electronic structures of the compounds have been examined by DFT calculations.- Published
- 2022
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558. Reversible Addition of Carbon Dioxide to Main Group Metal Complexes at Temperatures about 0 °C.
- Author
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Koptseva TS, Sokolov VG, Ketkov SY, Rychagova EA, Cherkasov AV, Skatova AA, and Fedushkin IL
- Abstract
The reaction of dialane [LAl-AlL] (1; L=dianion of 1,2-bis[(2,6-diisopropylphenyl)imino]acenaphthene, dpp-bian) with carbon dioxide results in two different products depending on solvent. In toluene at temperatures of about 0 °C, the reaction gives cycloadduct [L(CO
2 )Al-Al(O2 C)L] (2), whereas in diethyl ether, the reaction affords oxo-bridged carbamato derivative [L(CO2 )(Et2 O)Al(μ-O)AlL] (3). The DFT and QTAIM calculations provide reasonable explanations for the reversible formation of complex 2 in the course of two subsequent (2+4) cycloaddition reactions. Consecutive transition states with low activation barriers were revealed. Also, the DFT study demonstrated a crucial effect of diethyl ether coordination to aluminium on the reaction of dialane 1 with CO2 . The optimized structures of key intermediates were obtained for the reactions in the presence of Et2 O; calculated thermodynamic parameters unambiguously testify the irreversible formation of the product 3., (© 2021 Wiley-VCH GmbH.)- Published
- 2021
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559. Influence of the quick-to-court gene deletion on courtship behaviour of Drosophila melanogaster .
- Author
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G Belkina E, E Lazebny O, G Gornostaev N, S Mikhailov V, V Danilenkova L, G Besedina N, V Bragina J, G Kamyshev N, V Sokolov V, and I Kravchuk O
- Subjects
- Animals, Courtship, Drosophila melanogaster physiology, Female, Gene Deletion, Male, Mutation genetics, Sexual Behavior, Animal physiology, Behavior, Animal physiology, Drosophila Proteins genetics, Drosophila melanogaster genetics, Reproduction genetics
- Abstract
Using an original method, we have received Drosophila melanogaster with a deficiency including a complete sequence of quick-to-court gene. In this report, we describe the behavioural features of this new deletion mutant. There were no serious deviations from the normal mating behaviour in flies with the deletion, but the behaviour of deletion mutants still had some features. Of all the elements, only the frequency of licking significantly increased in mutants. The duration of mating elements did not change in flies with deletion, and the latent period decreased only for following the female and licking. We have found that mutant males produce more courtship song than control males when courting Oregon R females as estimated by the pulse song index. In our experiment, mutant females provoked much less pulse song production by Oregon R males than control females do. Moreover, Oregon R males initiate courtship song towards mutant females later than towards control females. In other words, the study of pulse song production showed that the deficiency in females leads to a decrease in the intensity of courtship of wild-type males, whereas the deficiency in males leads to more intensive care for wild-type females.
- Published
- 2021
560. Low-coordinate Sm(II) and Yb(II) complexes derived from sterically-hindered 1,2-bis(imino)acenaphthene (Ar BIG -bian).
- Author
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Lukina DA, Skatova AA, Sokolov VG, Baranov EV, Demeshko S, Ketkov SY, and Fedushkin IL
- Abstract
Reduction of ArBIG-bian (1, 1,2-bis[(2,6-dibenzhydryl-4-methylphenyl)imino]acenaphthene) with an excess of samarium in tetrahydrofuran (thf) and crystallization of the crude product from a thf/toluene mixture affords [(ArBIG-bian)Sm]·C7H8 (2a), which is free of the coordinating solvent. The use of 1,2-dimethoxyethane (dme) as the reaction medium resulted in the same product, [(ArBIG-bian)Sm]·C4H10O2 (2b), which differs from 2a only in the crystal lattice solvent. Reduction of 1 with an excess of ytterbium in dme gives compound [(ArBIG-bian)Yb(dme)]·2.5C7H8 (3), containing a coordinated dme molecule. All three products consist of dianionic ArBIG-bian ligands whose bulky 2,6-(Ph2CH)2-4-Me-C6H2 groups shield effectively the metal atoms. The newly prepared compounds 2a, 2b and 3 were characterized by 1H NMR and IR spectroscopy. The molecular structures of complexes 2a, 2b and 3 have been established by single crystal X-ray analysis. The intramolecular interactions were analysed on the basis of DFT calculations. The temperature dependence of the magnetic susceptibility of 2b and 3 in the region of 2-300 K was studied. The magnetic moments of complex 2b correspond to divalent samarium.
- Published
- 2020
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561. Pharmacological Sequestration of Mitochondrial Calcium Uptake Protects Neurons Against Glutamate Excitotoxicity.
- Author
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Angelova PR, Vinogradova D, Neganova ME, Serkova TP, Sokolov VV, Bachurin SO, Shevtsova EF, and Abramov AY
- Subjects
- Animals, Astrocytes drug effects, Astrocytes metabolism, Membrane Potential, Mitochondrial drug effects, Mitochondria metabolism, Neurons metabolism, Neuroprotective Agents pharmacology, Rats, Rats, Sprague-Dawley, Rats, Wistar, Calcium metabolism, Calcium Channels metabolism, Glutamic Acid pharmacology, Mitochondria drug effects, Neurons drug effects
- Abstract
Neuronal excitotoxicity which is induced by exposure to excessive extracellular glutamate is shown to be involved in neuronal cell death in acute brain injury and a number of neurological diseases. High concentration of glutamate induces calcium deregulation which results in mitochondrial calcium overload and mitochondrial depolarization that triggers the mechanism of cell death. Inhibition of mitochondrial calcium uptake could be potentially neuroprotective but complete inhibition of mitochondrial calcium uniporter could result in the loss of some physiological processes linked to Ca
2+ in mitochondria. Here, we found that a novel compound, TG-2112x, can inhibit only the lower concentrations mitochondrial calcium uptake (induced by 100 nM-5 μM) but not the uptake induced by higher concentrations of calcium (10 μM and higher). This effect was not associated with changes in mitochondrial membrane potential and cellular respiration. However, a pre-treatment of neurons with TG-2112x protected the neurons against calcium overload upon application of toxic concentrations of glutamate. Thus, sequestration of mitochondrial calcium uptake protected the neurons against glutamate-induced mitochondrial depolarization and cell death. In our hands, TG-2112x was also protective against ionomycin-induced cell death. Hence, low rate mitochondrial calcium uptake plays an underestimated role in mitochondrial function, and its inhibition could protect neurons against calcium overload and cell death in glutamate excitotoxicity.- Published
- 2019
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562. Pro-neurogenic, Memory-Enhancing and Anti-stress Effects of DF302, a Novel Fluorine Gamma-Carboline Derivative with Multi-target Mechanism of Action.
- Author
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Strekalova T, Bahzenova N, Trofimov A, Schmitt-Böhrer AG, Markova N, Grigoriev V, Zamoyski V, Serkova T, Redkozubova O, Vinogradova D, Umriukhin A, Fisenko V, Lillesaar C, Shevtsova E, Sokolov V, Aksinenko A, Lesch KP, and Bachurin S
- Subjects
- Animals, Fluorine Compounds metabolism, Indoles administration & dosage, Indoles chemistry, Male, Memory physiology, Mice, Mice, Inbred C57BL, Neurogenesis physiology, Rats, Receptors, Serotonin metabolism, Stress, Psychological metabolism, Stress, Psychological psychology, Carbolines administration & dosage, Drug Delivery Systems methods, Fluorine Compounds administration & dosage, Memory drug effects, Neurogenesis drug effects, Stress, Psychological drug therapy
- Abstract
A comparative study performed in mice investigating the action of DF302, a novel fluoride-containing gamma-carboline derivative, in comparison to the structurally similar neuroprotective drug dimebon. Drug effects on learning and memory, emotionality, hippocampal neurogenesis and mitochondrial functions, as well as AMPA-mediated currents and the 5-HT6 receptor are reported. In the step-down avoidance and fear-conditioning paradigms, bolus administration of drugs at doses of 10 or 40 mg/kg showed that only the higher dose of DF302 improved long-term memory while dimebon was ineffective at either dosage. Short-term memory and fear extinction remained unaltered across treatment groups. During the 5-day predation stress paradigm, oral drug treatment over a period of 2 weeks at the higher dosage regimen decreased anxiety-like behaviour. Both compounds supressed inter-male aggression in CD1 mice, the most eminent being the effects of DF302 in its highest dose. DF302 at the higher dose decreased floating behaviour in a 2-day swim test and after 21-day ultrasound stress. The density of Ki67-positive cells, a marker of adult neurogenesis, was reduced in the dentate gyrus of stressed dimebon-treated and non-treated mice, but not in DF302-treated mice. Non-stressed mice that received DF302 had a higher density of Ki67-positive cells than controls unlike dimebon-treated mice. Similar to dimebon, DF302 effectively potentiated AMPA receptor-mediated currents, bound to the 5-HT6 receptor, inhibited mitochondrial permeability transition and displayed cytoprotective properties in cellular models of neurodegeneration. Thus, DF302 exerts multi-target effects on the key mechanisms of neurodegenerative pathologies and can be considered as an optimized novel analogue of the neuroprotective agent dimebon.
- Published
- 2018
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563. Gallium Hydrides with a Radical-Anionic Ligand.
- Author
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Sokolov VG, Koptseva TS, Moskalev MV, Bazyakina NL, Piskunov AV, Cherkasov AV, and Fedushkin IL
- Abstract
The reaction of Cl
2 GaH with a sodium salt of the dpp-Bian radical-anion (dpp-Bian•- )Na (dpp-Bian = 1,2-bis[(2,6-diisopropylphenyl)imino]acenaphthene) affords paramagnetic gallane (dpp-Bian•- )Ga(Cl)H (1). Oxidation of (dpp-Bian2- )Ga-Ga(dpp-Bian2- ) (2) with N2 O results in the dimeric oxide (dpp-Bian•- )Ga(μ2 -O)2 Ga(dpp-Bian•- ) (3). A treatment of the oxide 3 with phenylsilane affords paramagnetic gallium hydrides (dpp-Bian•- )GaH2 (4) and (dpp-Bian•- )Ga{OSi(Ph)H2 }H (5) depending on the reagent's stoichiometry. The reaction of digallane 2 with benzaldehyde produces pinacolate (dpp-Bian•- )Ga(O2 C2 H2 Ph2 ) (6). In the presence of PhSiH3 , the reaction between digallane 2 and benzaldehyde (2: PhSiH3 : PhC(H)O = 1:4:4) affords compound 4. The newly prepared complexes 1, 3-6 consist of a spin-labeled diimine ligand-dpp-Bian radical-anion. The presence of the ligand-localized unpaired electron allows the use of the ESR spectroscopy for characterization of the gallium hydrides reported. The molecular structures of compounds 1, 3-6 have been determined by the single-crystal X-ray analysis.- Published
- 2017
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564. Burns and frostbite in the Red Army during World War II.
- Author
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Sokolov V, Biryukov A, Chmyrev I, Tarasenko M, and Kabanov P
- Subjects
- Burns therapy, Frostbite therapy, Humans, USSR epidemiology, Burns epidemiology, Frostbite epidemiology, Military Personnel statistics & numerical data, World War II
- Abstract
The start of World War II (WWII) led to the deployment of combat troops in several continents. Destruction and many casualties among both the military and civilians became an inevitable consequence. A large amount of people injured were in need of life-saving treatment and a speedy return to duty. Intensive studies of the specific issues of diagnosis and treatment of thermal injury were conducted in the Soviet Union before the war. The first special units for patients with burn injuries were created, and the first specialists received their first clinical experience. The contributions of famous Soviet scientists in the development of the treatment of burns and frostbite in WWII are studied in this article. The structure of thermal injuries among military personnel and the results of their treatment are shown. Treatment, classification and quantity frostbite in the structure of sanitary losses during the WWII are studied in this article.
- Published
- 2017
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565. Web application for automatic prediction of gene translation elongation efficiency.
- Author
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Sokolov V, Zuraev B, Lashin S, and Matushkin Y
- Subjects
- Animals, Humans, RNA, Messenger genetics, Gene Expression Regulation physiology, Internet, Models, Biological, Peptide Chain Elongation, Translational physiology, RNA, Messenger metabolism, Software
- Abstract
Expression efficiency is one of the major characteristics describing genes in various modern investigations. Expression efficiency of genes is regulated at various stages: transcription, translation, posttranslational protein modification and others. In this study, a special EloE (Elongation Efficiency) web application is described. The EloE sorts the organism's genes in a descend order on their theoretical rate of the elongation stage of translation based on the analysis of their nucleotide sequences. Obtained theoretical data have a significant correlation with available experimental data of gene expression in various organisms. In addition, the program identifies preferential codons in organism's genes and defines distribution of potential secondary structures energy in 5´ and 3´ regions of mRNA. The EloE can be useful in preliminary estimation of translation elongation efficiency for genes for which experimental data are not available yet. Some results can be used, for instance, in other programs modeling artificial genetic structures in genetically engineered experiments.
- Published
- 2015
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566. Adaptive behavior of a redox-active gallium carbenoid in complexes with molybdenum.
- Author
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Fedushkin IL, Sokolov VG, Piskunov AV, Makarov VM, Baranov EV, and Abakumov GA
- Abstract
A gallium(I) carbenoid derived from redox-active diimine 1,2-bis[(2,6-diisopropylphenyl)imino]acenaphthene (dpp-bian) in complexes with molybdenum may serve either as a neutral [(dpp-bian)Ga:] or an anionic [(dpp-bian)Ga:](-) two-electron donor depending on the electronic state of the transition metal.
- Published
- 2014
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567. Synthesis and biological activity of N-substituted-tetrahydro-γ-carbolines containing peptide residues.
- Author
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Sokolova NV, Nenajdenko VG, Sokolov VB, Vinogradova DV, Shevtsova EF, Dubova LG, and Bachurin SO
- Abstract
The synthesis of novel peptide conjugates of N-substituted-tetrahydro-γ-carbolines has been performed using the sequence of the Ugi multicomponent reaction and Cu(I)-catalyzed click chemistry. The effect of obtained γ-carboline-peptide conjugates on the rat liver mitochondria was evaluated. It was found that all compounds in the concentration of 30 µM did onot induce depolarization of mitochondria but possessed some inhibitory effect on the mitochondria permeability transition. The original N-substituted-tetrahydro-γ-carbolines containing an terminal alkyne group demonstrated a high prooxidant activity, whereas their conjugates with peptide fragments slightly inhibited both autooxidation and the t-BHP-induced lipid peroxidation.
- Published
- 2014
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568. CD4(+)CD25(high)CD127(low) regulatory T cells in patients with stable angina and their dynamics after intracoronary sirolimus-eluting stent implantation.
- Author
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Potekhina AV, Provatorov SI, Sokolov VO, Pylaeva EA, Masenko VP, Noeva EA, Kukhtina NB, Krasnikova TL, and Arefieva TI
- Subjects
- Aged, Angina Pectoris immunology, Angioplasty, Balloon, Laser-Assisted, C-Reactive Protein immunology, CD4-Positive T-Lymphocytes immunology, Female, Humans, Interleukin-2 Receptor alpha Subunit immunology, Interleukin-7 Receptor alpha Subunit immunology, Lymphocyte Subsets drug effects, Lymphocyte Subsets immunology, Male, Middle Aged, Risk Factors, Time Factors, Angina Pectoris pathology, Angina Pectoris therapy, Angioplasty, Balloon, Coronary, Drug-Eluting Stents, Immunologic Factors pharmacology, Sirolimus pharmacology, T-Lymphocytes, Regulatory drug effects, T-Lymphocytes, Regulatory immunology
- Abstract
Rapamycin contributes to the expansion of regulatory T cells (Tregs) in vitro. We investigated CD4(+)CD25(high)CD127(low) Treg level dynamics as well as the major parameters of cell immunity and sCD25 and highly sensitive C-reactive protein (hsCRP) concentrations in the blood of patients after coronary stenting (CS) with sirolimus (rapamycin)-eluting stents (SES; n = 43). The relation between initial Treg values and the severity of coronary atherosclerosis was observed. Treg and sCD25 levels were increased 1 month after CS versus baseline values and versus data in the control group (coronary angiography [CA], n = 20). A positive correlation between Treg and sCD25 levels was reported, whereas no relation was observed with the length of SES implanted. HsCRP level was increased during the first 7 days and returned to baseline values 1 month after CS/CA. Treg content is lower in patients with multivessel CAD. Elevated levels of Tregs and sCD25 after SES implantation might occur because of the immunomodulating effect of rapamycin., (Copyright © 2011 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.)
- Published
- 2011
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569. Molecular dynamics simulation of liquid methanol. I. Molecular modeling including C-H vibration and Fermi resonance.
- Author
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Ishiyama T, Sokolov VV, and Morita A
- Subjects
- Models, Molecular, Vibration, Methanol chemistry, Molecular Dynamics Simulation, Quantum Theory
- Abstract
A flexible and polarizable methanol model has been developed on the basis of charge response kernel (CRK) theory. The present CRK methanol model well reproduces bulk liquid and interfacial properties, including density, enthalpy of vaporization, diffusion coefficient, surface tension, and radial distribution functions. The modeling of intramolecular potential incorporates the anharmonic coupling effects pertinent to the Fermi resonance of stretching and bending overtones, with its effective quantum correction. Therefore, the present methanol model can describe the vibrational spectroscopic features of infrared, Raman, and sum frequency generation spectra of C-H or C-D stretching region of methanol or deuterated methanol on the same footing. This model allows for further detailed analysis of C-H vibrations of alkyl moieties by molecular dynamics simulation.
- Published
- 2011
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570. Molecular dynamics simulation of liquid methanol. II. Unified assignment of infrared, Raman, and sum frequency generation vibrational spectra in methyl C-H stretching region.
- Author
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Ishiyama T, Sokolov VV, and Morita A
- Subjects
- Models, Molecular, Spectrophotometry, Infrared, Spectrum Analysis, Raman, Vibration, Methanol chemistry, Molecular Dynamics Simulation
- Abstract
Vibrational spectra of methyl C-H stretching region are notoriously complicated, and thus a theoretical method of systematic assignment is strongly called for in condensed phase. Here we develop a unified analysis method of the vibrational spectra, such as infrared (IR), polarized and depolarized Raman, and ssp polarized sum frequency generation (SFG), by flexible and polarizable molecular dynamics simulation. The molecular model for methanol has been developed by charge response kernel model to allow for analyzing the methyl C-H stretching vibrations. The complicated spectral structure by the Fermi resonance has been unraveled by empirically shifting potential parameters, which provides clear information on the coupling mechanism. The analysis confirmed that for the IR, polarized Raman, and SFG spectra, two-band structure at about 2830 and 2950 cm(-1) results from the Fermi resonance splitting of the methyl C-H symmetric stretching and bending overtones. In the IR spectrum, the latter, higher-frequency band is overlapped with prominent asymmetric C-H stretching bands. In the depolarized Raman spectrum, the high frequency band at about 2980 cm(-1) is assigned to the asymmetric C-H stretching mode. In the SFG spectrum, the two bands of the splitted symmetric C-H stretching mode have negative amplitudes of imaginary nonlinear susceptibility χ(2), while the higher-frequency band is partly cancelled by positive imaginary components of asymmetric C-H stretching modes.
- Published
- 2011
- Full Text
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571. Acenaphthene-1,2-diimine chromium complexes.
- Author
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Fedushkin IL, Makarov VM, Sokolov VG, and Fukin GK
- Abstract
The reaction of (dpp-BIAN)Mg(THF)3 (1) with anhydrous CrCl3 in THF at 80 degrees C affords the mixed chromium-magnesium complex (dpp-BIAN)Cr(mu-Cl)3Mg(THF)3 (2). The treatment of 2 with 1,4-dioxane causes the elimination of MgCl2 and the formation of dimeric [(dpp-BIAN)Cr(mu-Cl)(THF)]2 (3). The reaction of (dpp-BIAN)Na2 with CrCl3 gives the chlorine-free compound [Na(THF)6][(dpp-BIAN)2Cr] (4). Paramagnetic complexes 2, 3 and 4 have been characterized by IR and electronic absorption spectroscopy. The magnetic susceptibilities of 2 and 4 have been determined in solution using Evans method. The molecular structures of 2-4 have been established by single crystal X-ray analysis.
- Published
- 2009
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572. Diethyl [2,2,2-trifluoro-1-phenyl-sulfonyl-amino-1-(trifluoro-meth-yl)eth-yl]phospho-nate.
- Author
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Wijeyesakere SJ, Nasser FA, Kampf JW, Aksinenko AY, Sokolov VB, Malygin VV, Makhaeva GF, and Richardson RJ
- Abstract
The title compound, C(13)H(16)F(6)NO(5)PS, is of inter-est with respect to inhibition of serine hydro-lases. Its structure contains a 1.8797 (13) Å P-C bond and two inter-molecular N-H⋯O=P hydrogen bonds, resulting in centrosymmetric dimers. An intra-molecular N-H⋯O=P hydrogen bond is also present.
- Published
- 2008
- Full Text
- View/download PDF
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