651. [Several carotenoids of vegetable determinated by high performance liquid chromatography].
- Author
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Zhao X, Pan L, Meng J, Wang Y, and et al
- Subjects
- Cryptoxanthins, Xanthophylls analysis, beta Carotene analysis, Carotenoids analysis, Chromatography, High Pressure Liquid methods, Vegetables chemistry
- Abstract
Objective: To detect alpha-carotene (alpha-C), beta-carotene (beta-C), cryptoxanthin (Cryp), lutein (Lut) and lycopene (Lyco) levels in vegetables., Methods: A HPLC method with C30 column was used to detect several carotenoids at the wave length of 450 nm. A gradient elution method with methanol, methyl-t-butyl-ether and ammonium acetate solution was used to separate the carotenoids., Results: The level of Lut, Cryp, alpha-c, beta-c and lycopene were linearly related with the peak area. The recoveries of the standard were between 94.7%-108.1%. The relative standard deviations were less than 4.62%., Conclusion: The method could be simple and credible. It could separate 5 carotenoids in vegetables simultaneously.
- Published
- 2008