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Objective: To investigate the effect of oral rehydration salts (ORS) combined with midodrine on serum electrolyte, expression levels of cortisol (Cor) and brain natriuretic peptide (BNP) in the treatment of vasovagal syncope (VVS). 90 VVS children admitted to department of pediatrics of our hospital from February 2019 to January 2021 were randomly divided into the matched group and research group, each group has 45 cases. Both groups were accepted with health education, the matched group was treated with midodrine, the research group was treated with midodrine combined with ORS. The clinical efficacy, negative rates, recurrence rates, HR and SBP, serum electrolyte levels, expression levels of Cor and BNP were combined, the adverse reactions during the research were recorded. The total effective rate of research group was higher than matched group (P<0.05). The negative rates of research group was higher than matched group, and the recurrence rate was lower than that in the control group (P<0.05). The HR and SBP of two group before and after the treatment had no difference (P＞0.05). The levels of serum sodium, calcium and chlorine of two groups after treatment were all increased, while phosphorus, potassium were decreased (P<0.05); as compared with matched group, the levels of serum sodium, calcium and chlorine of research group after treatment were higher, while phosphorus, potassium were lower(P<0.05). The expression levels of serum Cor and BNP of two groups after treatment were all decreased(P<0.05); as compared with matched group, the expression levels of serum Cor and BNP of research group after treatment were lower (P<0.05). There was no difference of incidence of adverse reactions of two groups during the research(P＞0.05). ORS combined with midodrine was effective in the treatment of VVS, the serum electrolyte was improved and the expression levels of serum Cor and BNP were decreased without obvious adverse reactions. [ABSTRACT FROM AUTHOR]

Objective To analyze the clinical features, diagnosis and treatment of 11 patients with livedoid vasculopathy. Methods The general data, clinical manifestations, dermoscopic findings, histopathological features and prognosis of diagnosis and treatment of 11 patients with livedoid vasculopathy were retrospectively analyzed. Results Among the 11 patients, there were 1 male and 10 females. The calf, ankle and dorsum were involved with erythema, purpura and blisters, followed by ulceration, ivory-white atrophy, scar and pigmentation after recovery. Five cases were severe in summer and mild in winter, and 6 cases were misdiagnosed as vasculitis and other diseases. Histopathological examination revealed fibrinoid necrosis and/or vascular embolism in dermal vessels. Five patients had common features of livedoid vasculopathy by dermoscopy. There was no recurrence in 6 patients who received combined treatment with oral glucocorticoid, rivaroxaban, dipyridamole, and aspirin, but 3 of 5 patients relapsed after single-drug treatment. Conclusions Livedoid vasculopathy is easy to be misdiagnosed clinically. When the ankle and dorsum of the foot are involved, the disease should be highly vigilant. Diagnosis is mainly based on histopathological changes, and dermoscopy can improve the diagnostic accuracy. Single-drug treatment is prone to recurrence, and combined drug treatment is effective. [ABSTRACT FROM AUTHOR]

Objective To investigate the genetic polymorphisms of 20 autosomal STR loci in Miao population from Wenshan and Honghe regions, and to explore their forensic values. Methods DNA was extracted with the Chelex-100 method. A total of 20 STR loci were amplified from 2 209 healthy unrelated individuals of the Miao population from Wenshan and Honghe regions, Yunnan Province using the PowerPlex®21 System kit. The PCR products were separated by capillary electrophoresis on an ABI 3130Genetic Analyzer, and alleles were genotyped by GeneMapper ID (Version 3.2) software. Forensic parameters were calculated by Modified-Powerstats software, and the Hardy-Weinberg equilibrium (HWE) test was performed. The Fst values and Nei's genetic distance were calculated by Arlequin and Phylip software, respectively. Multidimensional scaling (MDS) analysis was performed with SPSS v24.0 statistical software and the neighbor-joining (N-J) phylogenetic tree was constructed using the MEGA v6.0 software. Results A total of 265 alleles and 1 080 genotypes were observed. The allele frequency of the 20 STRs ranged from 0.000 2 to 0.5718. No deviation from Hardy-Weinberg equilibrium (P > 0.05/20 = 0.002 5) was observed. The cumulative discrimination power (CDP) and cumulative probability of exclusion (CPE) with 20 STRs were 0.999 999 999 999 999 999 999 932 674 151 and 0.999 999 935 545 335, respectively. Population comparison showed that Wenshan and Honghe Miao nationality was genetically closer to Yunnan Vietnamese. Conclusion These 23 STR loci are highly genetic polymorphic and informative in the Miao population from Wenshan and Honghe regions, Yunnan Province, which can be used for forensic identification and population genetic research. [ABSTRACT FROM AUTHOR]

Objective To review the data of carpal tunnel release surgery in the treatment of carpal tunnel syndrome (CTS) with carpal arthroscopy, and to explore the clinical effect of this operation. Methods 47 patients with carpal tunnel syndrome admitted in our department between December 2020 and December 2021 as the object in this study. All patients were treated with carpal tunnel syndrome by single-channel carpal tunnel release surgery under carpal arthroscopy, and all patients were followed up at least for 6 months. The recovery of symptoms and function before and after surgery was compared using the Modified Boston Carpal Tunnel Syndrome Score Scale (SSS score and FSS score）. Results After follow-up investigation, the symptoms of hand anesthesia and function in 47 patients were significantly improved compared with those before surgery, and the incidence of postoperative wrist pain was small. SSS score was ( 47.53±2.2) before and ( 82.13±9.42) after surgery, and the difference was statistically significant (P < 0.05). FSS score was (10.00±1.12) before and (30.76±2.64) after surgery, and the difference was statistically significant (P < 0.05). Conclusion Carpal arthroscopy single channel carpal tunnel release in the treatment of carpal tunnel syndrome can achieve reliable clinical efficacy, can significantly relieve patients with hand anesthesia and other symptoms, can reduce complication of postoperative pain and improve hand function, is worth popularizing in clinical use. [ABSTRACT FROM AUTHOR]

Objective: To investigate the genetic polymorphism of 18 autosomal short tandem repeats (STR) loci in 2 323 healthy unrelated individuals of Minjia branch of Bai nationality in Yunnan, calculate the population genetic parameters, and establish the genetic data of Minjia branch of Bai nationality in Yunnan, so as to provide the scientific basis for the parental identification and individual identification of forensic material evidence. Methods A total of 2 323 unrelated individuals from Minjia branch of Bai nationality population were collected. DNA TyperTM 19 kit was used for direct PCR compound amplification. AB 3130XL automatic genetic analyzer was used to separate the PCR compound amplification products by capillary electrophoresis. STR typing was performed with GeneMapper ID-x 1.5 software. Forensic parameters, such as allele frequency and matching probability, were calculated by Modified Powerstats software, and the hardy-Weinberg equilibrium (HWE) test was performed. The Fst and P values were calculated using Arlequin software. Nei’ s genetic distance was calculated using Phylip software. Multi-dimensional scale (MDS) analysis was performed using SPSS software. The Neighbor-Joining (NJ) phylogenetic tree was constructed by Mega software. Results A total of 230 alleles and 1 073 genotypes were observed at 18 STR loci. Except that D13S317 loci was not in accord with Hardy-Weinberg equilibrium ( P = 0.000 8）, the allele frequencies and genotype frequencies of other loci were consistent with Hardy-Weinberg equilibrium (P > 0.05/20 = 0.002 5）. The cumulative discrimination power (CDP) and cumulative probability of exclusion (CPE) of 18 STR loci were 0.999 999 999 and 0.999 999 940 55, respectively. The results of Fst, Nei’s genetic distance and NJ phylogenetic tree indicated that the Minjia branch of Bai nationality was close to the Bai nationality in Nujiang and the Bai nationality in Dali, but far away from the Bulang nationality in Yunnan and Va nationality in Yunnan. Conclusion The 18 STR loci mentioned above show the high genetic polymorphism in the Minjia branch of Bai nationality and they can be used for the forensic identification and population genetics research [ABSTRACT FROM AUTHOR]

Objective To observe the changes of superficial retinal microcirculation in macular area of 62 children and adolescents with myopia. Method We selected 62 children and adolescents with myopia (123 eyes) and 50 children and adolescents with normal vision (naked-eye vision ≥1. 0) (69 eyes), and compared the spherical equivalent degree (SE degree) and axial length (AL) of them. Sixty-two children and adolescents with myopia (123 eyes) were divided as follows：SE ≥ –0. 50 D (12 eyes), –0. 50 D> SE ≥–1. 00 D (10 eyes), –1. 00 D > SE ≥–3. 00 D (70 eyes), – 3. 00 D > SE (31 eyes) according to SE degree; AL > 25. 0 mm (36 eyes), 25. 0 mm≥AL > 24. 0mm (53 eyes), AL≤ 24. 0 mm (34 eyes) according to AL; retinal vascular length density (VLD), vascular perfusion density (VPD), macular fovea non-perfusion area (FAz-A), macular fovea non-perfusion area circumference (FAZ-P), macular fovea non-perfusion morphology index (FAZ-CI) were compared among all groups, and the correlation among SE, AL, VLD, VPD, FAZA, FAZ-P and FAZ-CI was analyzed in 62 children and adolescents with myopia (123 eyes) . Results Compared with normal children and adolescents, myopic children and adolescents had lower SE degree and longer AL (both P<0. 05) . There was no difference in the superficial retinal microcirculation in macular area with different SE degrees, but with the increase of myopia, the superficial retinal microcirculation in macular area decreased (P>0. 05) . There were statistically significant differences in the FAZ-A and FAZ-P in different AL (all P<0. 05), while no statistically significant differences were found in the other parameters (all P>0. 05) . However, numerically, with the prolongation of AL, except the central VLD and VPD, the other parameters showed a decreasing trend; statistically significant differences were found in FAZ-A and FAZ-P in the group with AL > 25. 0 mm and AL≤24. 0 mm, and in FAZ-A in the group with AL > 25. 0 mm and AL≤ 24. 0 mm (all P<0. 05) . SE degree was positively correlated with inner VPD, outer VPD and overall VPD (r=0. 183, 0. 206, 0. 194, respectively; all P<0. 05) ; there was no correlation between SE degree and central VLD, inner VLD, outer VLD, whole VLD, central VPD, FAZ-A, FAZ-P, and FAZ-CI (r=–0. 037, 0. 144, 0. 154, 0. 150, –0. 006, 0. 044, 0. 016, and 0. 049, respectively; all P>0. 05) . AL was positively correlated with central VLD and central VPD (r=0. 222 and 0. 187, both P<0. 05), and negatively correlated with FAZ-A, FAZ-P, and Faz-CI (r=–0. 305, –0. 224, and–0. 188, respectively; all P<0. 05), that was, with the increase of axial length, VLD and VPD in the center gradually increased, and the non-perfusion area of macular fovea decreased; there was no correlation between AL and inner VLD, outer VLD, whole VLD, inner VPD, outer VPD and whole VPD (r=–0. 120, –0. 090, –0. 068, –0. 174, –0. 166, and–0. 161, respectively; all P>0. 05) . There was a negative correlation between AL and SE degree (r=–0. 503, P<0. 05), indicating that the myopia degree increased and the ocular axis lengthened with the decrease of spherical equivalent. Conclusion With the increase of myopia degree and the extension of eye axis, the superficial retinal microcirculation in macular area of children and adolescents with myopia is reduced in different degrees, and there is superficial retinal microcirculation disorder in macular area, so improving the microcirculation disorder may control or delay the occurrence and development of myopia. [ABSTRACT FROM AUTHOR]

BACKGROUND: Exercise can improve people's cognitive ability, but the specific mechanism is not clear in the cerebellum. Studies have been reported that Orexin A is related to learning and memory. OBJECTIVE: To explore the effects of two different kinds of swimming exercise interventions on the spatial learning and memory ability and the expression of Orexin A in the rat cerebellum so as to explore the potential mechanism of exercise intervention effect on learning and memory abilities. METHODS: Thirty-six male Sprague-Dawley rats were randomly divided into three groups, including normal group (n=10), moderate load exercise group (n=13) and overload exercise group (n=13). We built the animal model by swimming. The rats in the normal group were fed normally for 8 weeks. The rats in moderate load exercise group were performed by the moderate load exercise intervention for 8 weeks, while those of the overload exercise group were subjected to overload exercise intervention for 8 weeks. The weights of three groups of rats were weighed every day. The 8-day Morris water maze test was performed to test the escape latency and the number of crossing the platform in the three groups of rats. Rat’s cerebellum and blood were collected 24 hours later. The serum testosterone level, Orexin A mRNA expression in the cerebellum and immunofluorescence expression of Orexin A in the cerebellum were detected. RESULTS AND CONCLUSION: On the 1st day, the moderate load exercise group took the shortest time to navigate the platform among the three groups of rats. The average escape latency of moderate load exercise group was significantly lower than that in the normal group (P < 0.01), while there was no significant difference in the average escape latency between overload exercise group and normal group (P > 0.05). Spatial exploration experiment was designed for 1 day, and the average number of crossing the platform in the moderate load exercise group was significantly higher than that in the normal group (P < 0.05), while the average number of crossing the platform in the overload exercise group was significantly lower than that in the normal group and moderate load group (P < 0.05, P < 0.01). The results of real-time quantitative PCR indicated that the expression of Orexin A mRNA in moderate load exercise group was significantly higher than that in the normal group (P < 0.01). Compared with the normal group, the expression of Orexin A mRNA in the overload exercise group was significantly lower (P < 0.05). Immunofluorescence assay revealed that Orexin A protein was mostly distributed in the cytoplasm of cerebellar nerve cells, surrounding the nucleus. Compared with the normal group, the mean absorbance value of Orexin A in the moderate load exercise group was significantly increased (P < 0.05), while the mean absorbance value of Orexin A in the overload exercise group was not significantly lower (P > 0.05). Therefore, long-term moderate load exercise training can improve the spatial learning and memory ability of rats, and the molecular mechanism may be the high expression of Orexin A in the cerebellum. Based on the intensity of moderate-load exercise training, with the increase of exercise load, long-term overload exercise training can decrease the expression of Orexin A mRNA in the cerebellum and the spatial memory ability of rats. [ABSTRACT FROM AUTHOR]

Objective To investigate the effect of leucine-rich-repeat-containing G-protein-coupled receptor 5 (LGR5) on the proliferation and drug resistance of HeLa cells and its possible mechanism. Methods LGR5 expression was interfered using shRNA, and LGR5 knockdown HeLa cells were constructed. The effect of LGR5 on the proliferation and drug resistance of HeLa cell was evaluated by cell count, clone formation and MTT; the expressions of LGR5 and β-catenin in HeLa cells were detected by Western blot method. Results LGR5 knockdown HeLa cell line was successfully constructed; the cell growth rate and clone formation rate in shLGR5 group were markedly decreased compared to those in shCon group (P<0.01). Drug resistance of HeLa to cisplatin differed significantly between shLGR5 group and shCon group (P<0.01). Moreover, the LGR5 knockdown inhibited the expression of β-catenin in HeLa cells. Conclusion LGR5 plays an important role in cell proliferation and drug resistance of HeLa cells, and its mechanism is related to Wnt/β-catenin signaling pathway. [ABSTRACT FROM AUTHOR]

OBJECTIVE: Whether a systematic review/meta-analysis is of high quality is the premise for the reliability of clinical evidence. However, there is still no quality evaluation of systematic review/meta-analysis regarding the walking training after spinal cord injury at home and abroad. This paper aims to evaluate the systematic reviews/meta-analysis regarding the effect of different gait training methods on walking function of patients with spinal cord injury by radar plotting, so as to provide intuitive basis for clinical research. METHODS: Nine Chinese or English databases were searched by computer for collecting systematical reviews/meta-analysis regarding gait training for spinal cord injury. The retrieval time was from inception to October 2019. Literature quality was analyzed from six dimensions: publication year, design type, AMSTAR-2 methodological quality score, PRISMA publication quality score, homogeneity and publication bias risk, and the rank average score was then calculated. For the evidence level of outcome indicators, GRADE tool was used to evaluate the included systematic reviews/meta-analysis. Excel 2016 was used to draw and optimize radar plot for further analysis. RESULTS: A total of 20 eligible literatures were included. Radar plots showed that the average scores on the year of publication, design type, AMSTAR-2 methodological quality score, PRISMA publication quality score, homogeneity, and publication bias risk were 11.50, 14.25, 10.90, 10.95, 14.85, and 14.95, respectively. The average rank score of all the included literatures was 12.90. The GRADE tool evaluation indicated that 17 outcome indicators were of low quality, 40 of medium quality, and only 2 of high quality. The main problems are the low quality of methodology and research report, such as the project failed to register in the international pre-registration database and explain the study protocol, did not explain the comprehensive and systematic retrieval strategy, did not report the included research content in detail, did not carry out heterogeneity test or did not explain the results. CONCLUSION: The quality of systematic reviews/meta-analysis literature regarding gait training of spinal cord injury is not high, and the quality of methodology and research report should be strengthened to further improve the quality of literature. The research and analysis should be conducted in strict accordance with the standards proposed by AMSTAR-2 and PRISMA. Most of the systematic reviews/meta-analyses included show that gait training can improve walking function of patients with spinal cord injury. Among them, orthosis therapy with higher literature quality and higher level of evidence is more effective for patients with spinal cord injury, and orthosis therapy can be used as much as possible in clinical practice. Radar plot is an intuitive, scientific and effective graphical evaluation method, which is worthy of popularization and application in rehabilitation medicine in the future. [ABSTRACT FROM AUTHOR]

Garlic (Allium sativum L.) is a biennial plant in the Liliaceae family. It is widely used as a condiment in cooking and daily life in the world, as well as Chinese herbal medicine. The garlic is rich in biologically active components, including allicin, polysaccharides, amino acids, and volatile oils, thereby mainly relieving fatigue and improve human immunity. Since the garlic has high water content in general, the quality is prone to deterioration during storage. Therefore, the dried garlic can inhibit the reproduction of spoilage microorganisms and chemical degradation, and further retain the original flavor of garlic, thereby extending the shelf life. At present, a commonly used technology of hot-air drying is characterized by the simple operation and large loading capacity, but the low drying efficiency and the long drying time has resulted in low quality of products after drying. In this study, a feasible pretreatment combined with the ultrahigh pressure (UHP) and ultrasound (US) was proposed to improve the drying process and quality of dried garlic slices. A low field nuclear magnetic resonance (LF-NMR) was selected to analyze the influence of pretreatments on the internal moisture migration during the drying process of garlic slices. The results found that the US or UHP during the hot air drying was beneficial to enhance the internal mass transfer of garlic slices, and thereby speed up the drying process. The US, UHP, and UHPs-US pretreatments resulted in a reduction in the drying time of garlic slices by 37.50%, 43.75%, and 62.50%, respectively, compared to the control. Six mathematical models were selected to fit the drying process of garlic slices. All three pretreatments reduced the energy consumption of drying, especially in the UHP-US, 32.31% lower than that with the untreated. The LF-NMR results showed that there were still three states of water in the garlic slices after pretreatments. Free water accounted for the largest proportion, followed by the non-flowing water, and the bound water was the least. In the drying characteristics, the free water was removed first in the drying process. All the US, UHP, and UHP-US reduced the removal time of free water. The UHP-US pretreatment took the shortest time (90 min) to remove the free water, indicating that the UHP-US was more conducive to weakening the binding of tissue structure to water for the better fluidity of water. Scanning electron microscopy (SEM) images revealed that the intercellular space of dehydrated garlic slices increased, and the cell wall was severely damaged after the UHP. The porous structure appeared in the dried garlic slices after the US and UHP-US pretreatments, where the cell wall contour became clear. The larger pore size indicated that the UHP-US was more contribute to improving the fluidity of water. The color difference of dried garlic slices was reduced after the three pretreatments, and the rehydration ratio and allicin content were significantly increased (P<0.05). Furthermore, the combined UHP-US pretreatment achieved the best quality of the dried garlic slices, with the color difference value of 16.11, while the allicin content of 2.67 mg/g, and the rehydration ratio of 2.90 g/g. The experimental findings can provide a theoretical reference for the potential technology of garlic drying with high efficiency, energy-saving, and high quality of dried garlic slices. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of calcium sensing receptor on cerebral ischemia-reperfusion (I/R)in rats. Methods: Sixty male Wistar rats, weighing 250-300 g, were randomly divided into three groups（n=20 each）using a random number table: sham operation group(group S), group I/R and calcium-sensing receptor antagonists group (group N). Animal models of middle cerebral artery occlusion/reperfusion in the later 2 groups were established by a filament method in the left external-internal carotid artery. Rats were injected with 1 mg/kg NPS-89636 in the calcium-sensing receptor antagonists group and the equal volume of dimethyl sulfoxide 10 minutes before the cerebral ischemia. Neurological function was assessed and scored at 24 h of reperfusion. The rats were sacrificed and then the brains were removed for determination of MDA content, SOD activity, apoptotic cell death was assessed by TUNEL staining， and the expression of Caspase-3 by immunohistochemical and Western blot. Results: The content of MDA, neuronal apoptosis index, expression levels of Caspase-3 positive cells and Caspase-3 protein in group I/R and N were higher than those in group S, and neurological function score and SOD activity were lower than those in group S(P<0.05); The content of MDA, neuronal apoptosis index, expression levels of Caspase-3 positive cells and Caspase-3 protein in group N were lower than those in group I/R, and neurological function score and SOD activity were higher than those in group I/R (P<0.05). Conclusion: Calcium-sensing receptor is involved in cerebral ischemia-reperfusion injury and cell apoptosis. [ABSTRACT FROM AUTHOR]

A mixed extractant composed of P204 and Cyanex272 was used to extract Co，Mn and Fe in laterite nickel leaching solution. The optimal extraction agent ratio, pH value of aqueous phase, extraction equilibration time, extraction temperature, and the most suitable acid concentration for back extraction were studied systematically. The results show that the optimum synergistic extraction is obtained under the conditions： the volume ratio of P204 : Cyanex272 : TBP : sulfonated kerosene is 5 : 15 : 5 : 75, pH value of the solution to be treated is 4. 5, the extraction temperature is 60 °C, and extraction time is 8 min. In the process of stripping, Co can be stripped effectively by sulfuric acid at a concentration o£ 50 g/L，Mn can be stripped effectively by sulfuric acid at a concentration of 110 g/L，and Fe can be stripped effectively by HC1 at a concentration of 7 mol/L. [ABSTRACT FROM AUTHOR]

In order to understand the electrochemical behavior of ascorbic acid organic acid detergent for chemical decontamination of nuclear power plant and improve its decontamination effect？ the main corrosion product (Fe3O4) in the pipelines of nuclear power plant was made into a chemically modified electrode by the fixing action of Nafion solution in this paper. The electrochemical behavior of Fe3O4i in ascorbic acid was studied by three-electrode system. The effects of scanning speed ？ solution concentration and reaction type were also investigated. At the same time, tests such as cyclic voltammetry, polarization curves and AC impedance were also performed. The electrochemical behavior of Fe3O4i in ascorbic acid was analyzed and a series of electrochemical data were obtained. [ABSTRACT FROM AUTHOR]

Molecular biology experiment training is essential for establishing the research ability. However, current teaching methods mainly focus on the practical ability and neglect thinking ability. In this study, we discussed the application of PBL teaching model in the biology experiment teaching by confirming that the idea "mutated β-catenin promotes colorectal cancer cell proliferation". Compared with the traditional method, in which DNA isolation, PCR, plasmid construction and purification, DNA electrophoresis, gel extraction, protein isolation, western blot and transfection are taught separately, PBL teaching model combines these different techniques to solve a scientific problem. The PBL teaching method delivered as much information as traditional method. In addition, the PBL method stressed the connection among these different techniques and their putative implication in research. With the PBL teaching method, the learning passion was greatly motivated. In summary, PBL teaching method significantly improves efficacy of teaching, which would provide some clues for biology experiment teaching reform. [ABSTRACT FROM AUTHOR]

Micro-organisms are the main drivers of water biogeochemical cycle and the major player in energy metabolism, which are pivotal for maintaining diversity and stability of the water ecological systems. Aerobic anoxygenic phototrophic bacteria (AAPB), the important functional groups widespread in the water, can acquire energy from light and further affect the hydrochemical composition. The importance of AAPB is well recognized and in-depth studied. In order to study the relationship between the hydrochemical characteristics and the AAPB feedback effect in front of the Wulixia Reservoir dam, water samples from different layers were collected. Based on the stable carbon isotope of dissolved organic carbon and particulate organic carbon isotope composition, the distribution pattern of AAPB were measured by real-time PCR technology. Results show that the hydrochemical type of the front of reservoir dam water system was HCO3--Ca2+-Mg2+ type and poor-moderate eutrophication state during the sampling period. The results of dissolved oxygen, stable carbon isotope and the C/N showed that the main source of organic carbon was produced by microorganisms. The ratio of AAPB to total planktonic bacteria in the Wulixia Reservoir water was 1.33%-1.60%, and the variation degree of AAPB abundance was greater than that of the total planktonic bacteria abundance, which means that the AAPB feedback are more sensitive to the hydrochemical characteristics compared with that of total planktonic bacteria. The canonical correspondence analysis can reveal the relationship between hydrochemical composition and AAPB. CCA result showed that AAPB and total planktonic bacteria were strongly influenced by turbidity, which makes it possible to apply marine microbiological pump theory in karst reservoirs. Hydrochemical characteristics feedback on AAPB will improve understanding of the metabolic system of CO2-H2O-carbonate and micro-organisms. [ABSTRACT FROM AUTHOR]

Objective To explore the expression of CX43 protein in endometrial polyps (EP). Methods We randomly selected 79 paraffin specimens of EP diagnosed at the Department of Obstetrics and Gynecology of the Second Affiliated Hospital, Xi'an Jiaotong University, from January 2011 to May 2013 after dilation and curettage of endometrial tissues by histopathological examination. They included 44 cases of EP tissues (18 cases in proliferational phase, 15 cases in secretory phase, and 11 cases in postmenopausal phase) and 35 cases of normal endometrial tissues as the control group (12 cases in proliferational phase, 12 cases in secretory phase, and 11 cases in postmenopausal phase). CX43 protein expression was analyzed in premenopausal and postmenopausal EP by immunohistochemical staining. Results The positive expression of CX43 protein in both control group and EP tissues was found in endometrial glandular epithelial cells and stroma cells surrounding the glands, principally in glandular epithelial cells. The positive rate of CX43 protein in the EP significantly decreased compared with control group in corresponding phase (P<0.05). The positive rate of CX43 protein in the proliferational and secretory stages was similar, but it decreased in postmenopausal stage compared to premenopausal phase of EP, but without significant difference (P>0.05). The positive expression of CX43 protein in EP had no correlation with hypertension and the number of polyps. Conclusion CX43 protein expression in endometrial polyps is reduced, which may play an important role in the development of EP. [ABSTRACT FROM AUTHOR]

Objective: To study the effect of autonomic nerve denervation on long-term efficaey of CPVA in atrial fibrillation patients. Methods: 104 consecutive paroxysmal AF(PAF) patients with symptomatic and drug refractory AF were enrolled in this study.All patients underwent CPVA. During follow-up,24 hour Holter monitoring was performed before and after procedure. Then the patients accepted Holter examination every 3 months. Free of atrial tachyarrhythmias(>30sec) during follow-up was regarded as no recurrence.Autonomic nerve denervation was assessed by heart rate variability(HRV) from Holter. Results: 104 patients finished the study.According to the outcome, the average operation time is(1.35 ±0.65), the overall cumulative success rate was 77.9 %, the time of follow-up is(24.27±11.13)-month. All patients were divided into success group(81 cases) and recurrence group(23 cases). After CPVA HRV decreased signiflcantly in each group. Furthermore,the characters of SDNN, r MSSD, PNN50, TF, VLF, LF and HF of success group were significantly lower than those of recurrence group(P<0.05). Conclusions: CPVA is an effective therapy for PAF, CPVA could induce autonomic nerve denervation, which may contribute to the long-term success of AF ablation. [ABSTRACT FROM AUTHOR]

Objective:To investigate the effects of valproic acid(VPA) and bortezomib(BZ) on cell proliferation and apoptosis in RPMI8226 cells. Methods: The drug groups were set as follows: control group; VPA alone group(1.0 mmol/L); BZ alone group A(10.0nmol/L); BZ alone group B(20.0 nmol/L); BZ alone group C(35.0 nmol/L); combination group A(VPA 1.0 mmol/L+BZ 10.0 nmol/L);combination group B(VPA 1.0 mmol/L+BZ 20.0 nmol/L); combination group C(VPA 1.0 mmol/L+BZ 35.0 nmol/L). The cell proliferation was tested by MTT and the apoptotic ratio was observed by flow cytometry. Results: Both valproic and bortezomib alone can inhibit the cell proliferation and induce the apoptosis in RPMI8226 cells; the cell death ratio was increased by 75.1%, and the apoptotic ratio was increased by 68.9%; when the cells were treated by valproic and bortezomib together, the effect was more obvious.Conclusions: The effect of valproic and bortezomib used together was significantly enhanced on promoting cell apoptosis and inhibiting the cell proliferation of RPMI8226 cells, suggesting that valproic plays a role. [ABSTRACT FROM AUTHOR]