51. Protective effects of sulforaphane on type 2 diabetes-induced cardiomyopathy via AMPK-mediated activation of lipid metabolic pathways and NRF2 function.
- Author
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Sun Y, Zhou S, Guo H, Zhang J, Ma T, Zheng Y, Zhang Z, and Cai L
- Subjects
- AMP-Activated Protein Kinases genetics, AMP-Activated Protein Kinases physiology, Animals, Cardiotonic Agents therapeutic use, Diabetes Mellitus, Experimental chemically induced, Diabetes Mellitus, Experimental complications, Diabetes Mellitus, Experimental drug therapy, Diabetes Mellitus, Type 2 chemically induced, Diabetes Mellitus, Type 2 drug therapy, Diabetic Cardiomyopathies genetics, Diabetic Cardiomyopathies pathology, Isothiocyanates therapeutic use, Lipid Metabolism drug effects, Lipid Metabolism genetics, Lipidoses genetics, Lipidoses metabolism, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, NF-E2-Related Factor 2 genetics, Signal Transduction drug effects, Signal Transduction genetics, Streptozocin, Sulfoxides, Cardiotonic Agents pharmacology, Diabetes Mellitus, Type 2 complications, Diabetic Cardiomyopathies prevention & control, Isothiocyanates pharmacology, Lipidoses prevention & control, NF-E2-Related Factor 2 physiology
- Abstract
Background: AMP-activated protein kinase (AMPK), particularly AMPKα2 isoform, plays a critical role in maintaining cardiac homeostasis. It was reported that sulforaphane (SFN) prevented type 2 diabetes (T2D)-induced cardiomyopathy accompanied by the activation of AMPK; In this study, AMPK's pivotal role in SFN-mediated prevention against T2D-induced cardiomyopathy was tested using global deletion of AMPKα2 gene (AMPKα2-KO) mice., Methods and Results: T2D was established by feeding 3-month high-fat diet (HFD) to induce insulin resistance, followed by an intraperitoneal injection of streptozotocin (STZ) to induce mild hyperglycemia in both AMPKα2-KO and wild-type (WT) mice. Then both T2D and control mice were subsequently treated with or without SFN for 3 months while continually feeding HFD or normal diet. Upon completion of the 3-month treatment, five mice from each group were sacrificed as a 3-month time-point (3 M). The rest continued normal diet or HFD until terminating study at the sixth month (6 M) of diabetes. Cardiac function was examined with echocardiography before sacrifice at both 3 M and 6 M. SFN prevented T2D-induced progression of cardiac dysfunction, remodeling (hypertrophy and fibrosis), inflammation, and oxidative damage in wild-type diabetic mice, but not in AMPKα2-KO mice. Mechanistically, SFN prevented T2D-induced cardiomyopathy not only by improving AMPK-mediated lipid metabolic pathways, but also enhancing NRF2 activation via AMPK/AKT/GSK3β pathway. However, these improving effects of SFN were abolished in AMPKα2-KO diabetic mice., Conclusions: AMPK is indispensable for the SFN-induced prevention of cardiomyopathy in T2D, and the activation of NRF2 by SFN is mediated by AMPK/AKT/GSK3β signaling pathways., (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Published
- 2020
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