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The bee venom is used for treating a wide variety of disease conditions. It has also been found to be a strong immunological agent, stimulating the body's protective mechanisms against disease. The major treatment is gene therapy or recombinant DNA vaccines involved targeting multiple antigenic components to direct and empower the immune system to protect the host from infection. Limitation of therapy to the treatment of patients suffering from various adverse reaction and contraindications are always experienced. Tertiapin is a neurotoxin from the honeybee venom. It interacts specifically with calmodulin in the presence of Ca2+. Antigenic epitopes on Tertiapin protein of Apis mellifera (honey bee) is important determinants sites for protection against disorders. As our knowledge of the immune responses to a protein antigen progressed, it became clear that the whole protein is not necessary for raising the immune response, but small segments (NCNRIIIPHMCWK, 4-16) of tertiapin protein called the antigenic determinants or the epitopes are sufficient for eliciting the desired immune response. Immunization cassettes should be capable of immunizing of broad immunity against both humoral and cellular epitope thus giving vaccines the maximum ability to deal with Tertiapin protein of Apis mellifera immune escape. We have predicted a successful immunization strategy. KeywordsTertiapin, apitherapy, Antigenic epitope, Hydrophobicity, Hydrophilicity Introduction Apitherapy, or bee therapy, is the use of products of the common honeybee for therapeutic purposes. Honeybee venom, bee pollen, raw honey, royal jelly, and propolis are the products generally considered to have medicinal effects. These products are said to be effective against a wide range of ailments, from arthritis and chronic pain to multiple sclerosis and cancer, although few scientific studies have as yet proved their benefits. Bee venom is a potent anti-inflammatory antioxidant, antifungal, antibacterial, and possesses radioprotectant actions. It has been found to exert powerful actions as an antibacterial agent, anti-inflammatory, antiarthritic, antirheumatic, in neurodegenerative disease, as a cardiotonic, an antioxidant, and as a diaphoretic and diuretic. It has also been found to be a strong immunological agent, stimulating the body's protective mechanisms against disease. The Eclectic Botanical physicians considered it to be a potent alterative.

Chemical composition, antioxidant potential and corresponding lipid preoxidation of Indian commercial beers were evaluated. The presence of polyphenolic compounds such as tannic acid, gallic acid, catechol, vanillin, caffeic acid, quercetin, p-coumaric acid and rutin was quantified using LC-MS while the organic acids including tartaric, malic, acetic, citric and succinic acids were analysed using HPLC. Beer sample B8 had the greatest concentration of phenolic and flavonoid components (0.620 ± 0.084 mg/mL and 0.379 ± 0.020 mg/mL respectively) among the beer samples studied. The DPPH radical scavenging activity was observed in the range of 68.34 ± 0.85 % to 89.90 ± 0.71 % and ABTS radical cation scavenging activity was in the range of 59.75 ± 0.20 % to 76.22 ± 0.50 %. Percent protection in lipid peroxidation was quantified to be maximum (54.45 ± 3.39 %) in sample B5. Total phenolic content positively correlates with antioxidant assays, DPPH and ABTS (r = 0.35 and r = 0.58 respectively) with p