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A manatee’s primary modality to detect a vessel on a possible collision course is hearing as underwater visibility is limited in many manatee habitats and their visual acuity is poor. We estimate a Florida manatee’s ability to detect the sound of an approaching boat and vocalizations in four different soundscapes in Sarasota Bay, FL. Background noise samples were collected every 5 minutes for a two-week period during winter and summer at each location (2019 or 2020). Sound levels in third octave bands (0.5, 1, 2, 4, and 8 kHz) were measured and compared to manatee auditory hearing thresholds and to sound levels of an approaching boat traveling at a slow, medium, or fast speed. Background sound levels in a wider band (1–20 kHz) were calculated to model vocal communication space at each location. We found that a manatee’s estimated ability to detect an approaching boat differs greatly among locations, with time of day, and by season, and that fast boats are predicted to be detected later than slow boats. Latency of boat noise detection is estimated to sharply increase when considering unusually loud background noise levels. We suggest that such uncommonly loud conditions (e.g. 95th percentile sound level), not just typical conditions (median sound level), are important to consider for understanding the problem of manatee-boat collisions. Additionally, background noise impacts estimated vocal communication space and may limit the ability of vocal-mediated mother-calf cohesion. Altogether, a manatee’s ability to detect acoustic signals of interest is expected to vary greatly spatially and temporally.

We present the discovery of a new double detonation progenitor system consisting of a hot subdwarf B (sdB) binary with a white dwarf companion with an P=76.34179(2) min orbital period. Spectroscopic observations are consistent with an sdB star during helium core burning residing on the extreme horizontal branch. Chimera light curves are dominated by ellipsoidal deformation of the sdB star and a weak eclipse of the companion white dwarf. Combining spectroscopic and light curve fits we find a low mass sdB star, $M_{\rm sdB}=0.383\pm0.028$ M$_\odot$ with a massive white dwarf companion, $M_{\rm WD}=0.725\pm0.026$ M$_\odot$. From the eclipses we find a blackbody temperature for the white dwarf of 26,800 K resulting in a cooling age of $\approx$25 Myrs whereas our MESA model predicts an sdB age of $\approx$170 Myrs. We conclude that the sdB formed first through stable mass transfer followed by a common envelope which led to the formation of the white dwarf companion $\approx$25 Myrs ago. Using the MESA stellar evolutionary code we find that the sdB star will start mass transfer in $\approx$6 Myrs and in $\approx$60 Myrs the white dwarf will reach a total mass of $0.92$ M$_\odot$ with a thick helium layer of $0.17$ M$_\odot$. This will lead to a detonation that will likely destroy the white dwarf in a peculiar thermonuclear supernova. PTF1 2238+7430 is only the second confirmed candidate for a double detonation thermonuclear supernova. Using both systems we estimate that at least $\approx$1% of white dwarf thermonuclear supernovae originate from sdB+WD binaries with thick helium layers, consistent with the small number of observed peculiar thermonuclear explosions., accepted for publication in ApJL, 14 pages, 5 figures, 1 table

AIMS We aimed to describe the effectiveness of Onabotulinumtoxin A (Botox) in children with neurogenic bladder (NGB) unresponsive to medical therapy to determine urodynamic parameters predictive of success. METHODS Children receiving Botox for refractory NGB, between 2008 and 2019, from a single academic center, were included in this study. Botox success was defined as improvement of incontinence and/or urodynamic parameters. RESULTS Of 34 patients who received Botox, 13 (38.2%) had a positive response from their first injection, with improvement in capacity by a median of 35% of expected capacity for age compared to only a 9% increase in those who did not respond clinically. When patients were divided into groups by baseline urodynamic parameters, high-pressure (Pdetmax > 20 cm H2 O) patients had significantly greater improvement in compliance compared with low-pressure patients (p = 0.017). Low compliance patients (

Bauer, Sacher, Habig und Fechner widmen sich in diesem Beitrag der Frage, wie konventionelle Laborpraktika durch unterschiedliche eLearning-Formate angereichert werden können. Laborpraktika stellen in naturwissenschaftlichen Fächern einen elementaren Bestandteil des Curriculums dar. Die Studierenden erlernen in diesem Lehr-Lernformat das universitäre Experimentieren. In diesem Beitrag werden zwei Beispiele vorgestellt, wie konventionelle Laborpraktika mit eLearning-Angeboten ergänzt werden können. Auf Basis der Evaluation werden die Potenziale und Grenzen der unterschiedlichen eLearning-Bestandteile vor dem Hintergrund aktueller Entwicklungen digitaler Experimentierumgebungen sowie der Lernwirksamkeit analysiert. Basierend auf den Erkenntnissen werden Empfehlungen zur Weiterentwicklung von Laborpraktika mittels Augmented bzw. Virtual Reality abgeleitet.

BackgroundCongenital anomalies of the kidneys and urinary tract (CAKUT) constitute the most common cause of chronic kidney disease in the first three decades of life. Variants in four Forkhead box (FOX) transcription factors have been associated with CAKUT. We hypothesized that other FOX genes, if highly expressed in developing kidneys, may also represent monogenic causes of CAKUT.MethodsWe here performed whole-exome sequencing (WES) in 541 families with CAKUT and generated four lists of CAKUT candidate genes: (A) 36 FOX genes showing high expression during renal development, (B) 4 FOX genes known to cause CAKUT to validate list A, (C) 80 genes that we identified as unique potential novel CAKUT candidate genes when performing WES in 541 CAKUT families and (D) 175 genes identified from WES as multiple potential novel CAKUT candidate genes.ResultsTo prioritize potential novel CAKUT candidates in the FOX gene family, we overlapped 36 FOX genes (list A) with lists C and D of WES-derived CAKUT candidates. Intersection with list C identified a de novo FOXL2 in-frame deletion in a patient with eyelid abnormalities and ureteropelvic junction obstruction, and a homozygous FOXA2 missense variant in a patient with horseshoe kidney. Intersection with list D identified a heterozygous FOXA3 missense variant in a CAKUT family with multiple affected individuals.ConclusionsWe hereby identified FOXL2, FOXA2 and FOXA3 as novel monogenic candidate genes of CAKUT, supporting the utility of a paralog-based approach to discover mutated genes associated with human disease.

We report the discovery of SDSS J133725.26+395237.7 (hereafter SDSS J1337+3952), a double-lined white dwarf (WD+WD) binary identified in early data from the fifth generation Sloan Digital Sky Survey (SDSS-V). The double-lined nature of the system enables us to fully determine its orbital and stellar parameters with follow-up Gemini spectroscopy and Swift UVOT ultraviolet fluxes. The system is nearby ($d = 113$ pc), and consists of a $0.51\, M_\odot$ primary and a $0.32\, M_\odot$ secondary. SDSS J1337+3952 is a powerful source of gravitational waves in the millihertz regime, and will be detectable by future space-based interferometers. Due to this gravitational wave emission, the binary orbit will shrink down to the point of interaction in $\approx 220$ Myr. The inferred stellar masses indicate that SDSS J1337+3952 will likely not explode as a Type Ia supernova (SN Ia). Instead, the system will probably merge and evolve into a rapidly rotating helium star, and could produce an under-luminous thermonuclear supernova along the way. The continuing search for similar systems in SDSS-V will grow the statistical sample of double-degenerate binaries across parameter space, constraining models of binary evolution and SNe Ia., Accepted to ApJ

Recently, a class of Roche-lobe-filling binary systems consisting of hot subdwarf stars and white dwarfs with sub-hour periods has been discovered. At present, the hot subdwarf is in a shell He burning phase and is transferring some of its remaining thin H envelope to its white dwarf companion. As the evolution of the hot subdwarf continues, it is expected to detach, leaving behind a low mass C/O core white dwarf secondary with a thick He layer. Then, on a timescale of $\sim 10$ Myr, gravitational wave radiation will again bring the systems into contact. If the mass transfer is unstable and results in a merger and a catastrophic thermonuclear explosion is not triggered, it creates a remnant with a C/O-dominated envelope, but one still rich enough in He to support an R Corona Borealis-like shell burning phase. We present evolutionary calculations of this phase and discuss its potential impact on the cooling of the remnant white dwarf., 7 pages, 4 figures; Accepted to ApJ

International audience; Congenital anomalies of the kidney and urinary tract (CAKUT) constitute one of the most frequent birth defects and represent the most common cause of chronic kidney disease in the first three decades of life. Despite the discovery of dozens of monogenic causes of CA-KUT, most pathogenic pathways remain elusive. We performed whole-exome sequencing (WES) in 551 individuals with CAKUT and identified a heterozygous de novo stop-gain variant in ZMYM2 in two different families with CAKUT. Through collaboration, we identified in total 14 different heterozygous loss-of-function mutations in ZMYM2 in 15 unrelated families. Most mutations occurred de novo, indicating possible interference with reproductive function. Human disease features are replicated in X. tropicalis larvae with morpho-lino knockdowns, in which expression of truncated ZMYM2 proteins, based on individual mutations, failed to rescue renal and cranio-facial defects. Moreover, heterozygous Zmym2-deficient mice recapitulated features of CAKUT with high penetrance. The ZMYM2 protein is a component of a transcriptional corepressor complex recently linked to the silencing of developmentally regulated endoge-nous retrovirus elements. Using protein-protein interaction assays, we show that ZMYM2 interacts with additional epigenetic silencing complexes, as well as confirming that it binds to FOXP1, a transcription factor that has also been linked to CAKUT. In summary, our findings establish that loss-of-function mutations of ZMYM2, and potentially that of other proteins in its interactome, as causes of human CAKUT, offering new routes for studying the pathogenesis of the disorder.

Two sets of macrocyclic, bio-inspired, non-heme ligands are utilized for the synthesis of NiII, PdII and PtII complexes. The ligands consist of a 16-atom macrocycle, formed by four methylene bridged NHC moieties, with imidazole or benzimidazole as building blocks. The complexes exhibit a square planar coordination geometry and are characterized by NMR, ESI-MS, elemental analysis, SC-XRD and UV/Vis. For complexes incorporating benzimidazole, an evaluation of luminescence properties is performed, and is found that phosphorescence is present for the PdII derivative and there is fluorescence for the PtII derivative. Stability studies in cell culture medium are performed for subsequent MTT assays. Here, the NiII complexes show low to no activity, and PdII and PtII complexes exhibit remarkable low IC50 values in cisplatin resistant A2780cisR cells.

Carbohydrates are a large class of natural products that play key roles in a number of biological processes such as in cellular communication or disease progression. Carbohydrates are also used as vaccines and pharmaceuticals. Their synthesis through glycosylation reactions is challenging, and often stoichiometric amounts of promoters are required. Transition metal catalyzed glycosylation reactions are far less common, but can have advantages with respect to reaction conditions and selectivity. The review intends to approach the topic from the catalysis and carbohydrate perspective to encourage researchers from both the fields to perform research in the area. The article covers the basics in glycosylation and catalysis chemistry. The catalysts for the reaction can be roughly divided into two groups. In one group, the catalysts serve as Lewis acids. In the other group, the catalysts play a higher sophisticated role, are involved in all elementary steps of the mechanism and remain coordinated to the substrate throughout the whole catalytic cycle. Based on selected examples, the main trends in transition metal catalyzed glycosylation reactions are explained. Lewis acid catalysts tend to require a somewhat higher catalyst load compared to other organometallic catalysts. The reaction conditions such as the temperature and time depend in many cases on the leaving group employed. An outlook is also presented. The article is not meant to be comprehensive; it outlines the most common transition metal catalyzed processes with the intention to bring the catalysis and carbohydrate communities together and to inspire research activities in both areas.

Congenital anomalies of the kidney and urinary tract (CAKUT) are the most common cause of chronic kidney disease in the first three decades of life, and in utero obstruction to urine flow is a frequent cause of secondary upper urinary tract malformations. Here, using whole-exome sequencing, we identified three different biallelic mutations in CHRNA3, which encodes the α3 subunit of the nicotinic acetylcholine receptor, in five affected individuals from three unrelated families with functional lower urinary tract obstruction and secondary CAKUT. Four individuals from two families have additional dysautonomic features, including impaired pupillary light reflexes. Functional studies in vitro demonstrated that the mutant nicotinic acetylcholine receptors were unable to generate current following stimulation with acetylcholine. Moreover, the truncating mutations p.Thr337Asnfs(∗)81 and p.Ser340(∗) led to impaired plasma membrane localization of CHRNA3. Although the importance of acetylcholine signaling in normal bladder function has been recognized, we demonstrate for the first time that mutations in CHRNA3 can cause bladder dysfunction, urinary tract malformations, and dysautonomia. These data point to a pathophysiologic sequence by which monogenic mutations in genes that regulate bladder innervation may secondarily cause CAKUT.

Although rhenium (Re) is a rare element, it is an economically important one due to its use as a catalyst in the chemical preparation of gasoline and in technologically vital alloys. While the cytotoxicity of specific rhenium compounds has been tested against various cell lines, there is a dearth of information about the toxicity of Re-compounds, since Re is considered a nontoxic element. However, recent studies with Re-compounds as anticancer agents have demonstrated that some these compounds are effective in combating cancer, and therefore it is important to examine the toxicity of Re-compounds in the environment. In addition, information regarding speciation of Re-compounds, the assays involved in testing the toxicity of the compounds, and the available biological studies are provided. Re has been used in biological studies as a radiotracer, due in part to the ease in producing 188Re and recently 186Re. Re has some properties that are similar to technetium and is being used with increasing frequency to replace technetium (Tc) or in conjunction with Tc. This review describes the toxicity of Re materials reported in the literature. In addition, these reported toxicity studies are placed in the context of ecosystems showing ecotoxicity, as well as describing speciation and subsequently toxicity of various species in a variety of cytotoxicity assays.

This review focuses on cytotoxic rhenium compounds in terms of IC50 values, their mode of action in biological systems and their status in clinical trials. Biological studies of different rhenium compounds ordered by the oxidation state of rhenium are presented. Numerous rhenium complexes are reported, with the greatest number of compounds containing a Re(I)(CO)3+ core. A wide range of complexes has been designed using a combination of organometallic ligands, N- or S-based ligands, peptides, multidentate ligands and oxo groups. Design concepts based on membrane permeability and lipophilicity, membrane receptor targets and specific enzyme targets are presented. The cytotoxicity parameter IC50 is shown for organometallic compounds, coordination complexes, clusters and Re(oxo) complexes. In addition, a brief summary of in vivo studies is given. A further summary of rhenium compounds subjected to clinical trials is presented to provide information about the classes of rhenium compounds that have been tested in human beings and the approaches used in these studies. Moreover, the comparability of the IC50 values among the cytotoxicity studies is critically assessed to provide the basis for a summary of the most potent rhenium compounds according to their reported IC50 values for each type of cancer. The summary of the structures for the most cytotoxic complexes allows the identification of structural similarities and basic features that could lead to their cytotoxicity and might be useful for future investigations. Finally, the information from the analysis of the rhenium compounds subjected to cellular studies is compared to data on the rhenium compounds that have been involved in in vivo evaluation and clinical trials.

Binary systems of a hot subdwarf B (sdB) star + a white dwarf (WD) with orbital periods less than 2-3 hours can come into contact due to gravitational waves and transfer mass from the sdB star to the WD before the sdB star ceases nuclear burning and contracts to become a WD. Motivated by the growing class of observed systems in this category, we study the phases of mass transfer in these systems. We find that because the residual outer hydrogen envelope accounts for a large fraction of an sdB star's radius, sdB stars can spend a significant amount of time ($\sim$10s of Myr) transferring this small amount of material at low rates ($\sim 10^{-10}$-$10^{-9}\ M_\odot\,\rm yr^{-1}$) before transitioning to a phase where the bulk of their He transfers at much faster rates ($\gtrsim 10^{-8}\ M_\odot\,\rm yr^{-1}$). These systems therefore spend a surprising amount of time with Roche-filling sdB donors at orbital periods longer than the range associated with He star models without an envelope. We predict that the envelope transfer phase should be detectable by searching for ellipsoidal modulation of Roche-filling objects with $P_{\rm orb}=30$-$100$ min and $T_{\rm eff}=20{,}000$-$30{,}000$ K, and that many ($\geq$10) such systems may be found in the Galactic plane after accounting for reddening. We also argue that many of these systems may go through a phase of He transfer that matches the signatures of AM CVn systems, and that some AM CVn systems associated with young stellar populations likely descend from this channel., 16 pages, 8 figures, accepted for publication in ApJ

Purpose Congenital anomalies of the kidneys and urinary tract (CAKUT) constitute the leading cause of chronic kidney disease in children. In total, 174 monogenic causes of isolated or syndromic CAKUT are known. However, syndromic features may be overlooked when the initial clinical diagnosis of CAKUT is made. We hypothesized that the yield of a molecular genetic diagnosis by exome sequencing (ES) can be increased by applying reverse phenotyping, by re-examining the case for signs/symptoms of the suspected clinical syndrome that results from the genetic variant detected by ES. Methods We conducted ES in an international cohort of 731 unrelated families with CAKUT. We evaluated ES data for variants in 174 genes, in which variants are known to cause isolated or syndromic CAKUT. In cases in which ES suggested a previously unreported syndromic phenotype, we conducted reverse phenotyping. Results In 83 of 731 (11.4%) families, we detected a likely CAKUT-causing genetic variant consistent with an isolated or syndromic CAKUT phenotype. In 19 of these 83 families (22.9%), reverse phenotyping yielded syndromic clinical findings, thereby strengthening the genotype–phenotype correlation. Conclusion We conclude that employing reverse phenotyping in the evaluation of syndromic CAKUT genes by ES provides an important tool to facilitate molecular genetic diagnostics in CAKUT.

In this study, a new species of Pseudogilbia Møller, SchwarzhansNielsen 2004 is described based on two male specimens (40-44 mm L

The Diels–Alder cycloaddition between bisdienes and bisdienophile incorporating the 7-oxa-bicyclo[2.2.1]heptane unit are well known to show high diastereoselectivity that can be exploited for the synthesis of molecular belts. The related bisdiene 5,6,7,8-tetramethylidene-2-bicyclo[2.2.2]octene is a valuable building block for the synthesis of photoprecursors for acenes, but it has not been employed for the synthesis of molecular belts. The present work investigates by computational means the Diels–Alder reaction between these bisdiene building blocks with syn-1,4,5,8-tetrahydro-1,4:5,8-diepoxyanthracene, which shows that the diastereoselectivity of the Diels–Alder reaction of the etheno-bridged bisdiene is lower than that of the epoxy-bridged bisdiene. The reaction of the etheno-bridged bisdiene and syn-1,4,5,8-tetrahydro-1,4:5,8-diepoxyanthracene in 2:1 ratio yields two diastereomers that differ in the orientation of the oxa and etheno bridges based on NMR and X-ray crystallography. The all-syn diastereomer can be transformed into a molecular belt by inter- and intramolecular Diels–Alder reactions with a bifunctional building block. The molecular belt could function as a synthetic intermediate en route to a [11]cyclacene photoprecursor.

Stellar evolution and numerical hydrodynamics simulations depend critically on access to fast, accurate, thermodynamically consistent equations of state. We present Skye, a new equation of state for fully-ionized matter. Skye includes the effects of positrons, relativity, electron degeneracy, Coulomb interactions, non-linear mixing effects, and quantum corrections. Skye determines the point of Coulomb crystallization in a self-consistent manner, accounting for mixing and composition effects automatically. A defining feature of this equation of state is that it uses analytic free energy terms and provides thermodynamic quantities using automatic differentiation machinery. Because of this, Skye is easily extended to include new effects by simply writing new terms in the free energy. We also introduce a novel thermodynamic extrapolation scheme for extending analytic fits to the free energy beyond the range of the fitting data while preserving desirable properties like positive entropy and sound speed. We demonstrate Skye in action in the MESA stellar evolution software instrument by computing white dwarf cooling curves., Accepted in ApJ. 27 pages, 19 figures

Learning about the nature of science is an important aspect of becoming part of the culture of practicing physicists. While these topics have a long-standing tradition in U.S. curricula they are currently not part of German educational standards or curricula. And while there is some research concerning primary and secondary school students’ beliefs in this area, there are hardly any data about German university students. In this study we query 100 physics students in their first to fifth semester at a German university for their views of nature of science. We then compare them to a sample of physics professors at German universities from an earlier study. The students exhibit quite adequate views in some areas but could improve on their knowledge of the variability of scientific methods and the tentativeness and limited certainty of scientific knowledge—two issues they have in common with the professors. If we interpret the sample longitudinally, we see positive development in the first but worsening in the latter area.

White dwarfs with metal-polluted atmospheres have been studied widely in the context of the accretion of rocky debris from evolved planetary systems. One open question is the geometry of accretion and how material arrives and mixes in the white dwarf surface layers. Using the 3D radiation-hydrodynamics code CO$^5$BOLD, we present the first transport coefficients in degenerate star atmospheres which describe the advection-diffusion of a passive scalar across the surface-plane. We couple newly derived horizontal diffusion coefficients with previously published vertical diffusion coefficients to provide theoretical constraints on surface spreading of metals in white dwarfs. Our grid of 3D simulations probes the vast majority of the parameter space of convective white dwarfs, with pure-hydrogen atmospheres in the effective temperature range 6000-18000 K and pure-helium atmospheres in the range 12000-34000 K. Our results suggest that warm hydrogen-rich atmospheres (DA; $\gtrsim$13000 K) and helium-rich atmospheres (DB, DBA; $\gtrsim$30000 K) are unable to efficiently spread the accreted metals across their surface, regardless of the time dependence of accretion. This result may be at odds with the current non-detection of surface abundance variations at white dwarfs with debris discs. For cooler hydrogen- and helium-rich atmospheres, we predict a largely homogeneous distribution of metals across the surface within a vertical diffusion timescale. This is typically less than 0.1 per cent of disc lifetime estimates, a quantity which is revisited in this paper using the overshoot results. These results have relevance for studies of the bulk composition of evolved planetary systems and models of accretion disc physics., Comment: Accepted for publication in MNRAS. 22 pages, 17 figures

Mit dem Bologna-Prozess hat in der deutschen Hochschullandschaft ein Wandel der Lernorientierung weg von der Content- hin zu einer Outcome-Orientierung begonnen. Laborpraktika können diesem Wandel aufgrund des hohen Praxisanteils besonders gut gerecht werden. Anhand des neu konzipierten, kompetenzorientierten Paderborner Physik Praktikums 3P wird in dem Beitrag beispielhaft dargestellt, wie Lehrende in ihren Lehrveranstaltungen den veränderten Kompetenzerwartungen gerecht werden können.

Macphail’s comparative approach to intelligence focused on associative processes, an orientation inconsistent with more multifaceted lay and scientific understandings of the term. His ultimate emphasis on associative processes indicated few differences in intelligence among vertebrates. We explore options more attuned to common definitions by considering intelligence in terms of richness of representations of the world, the interconnectivity of those representations, the ability to flexibly change those connections, and knowledge. We focus on marine mammals, represented by the amphibious pinnipeds and the aquatic cetaceans and sirenians, as animals that transitioned from a terrestrial existence to an aquatic one, experiencing major changes in ecological pressures. They adapted with morphological transformations related to streamlining the body, physiological changes in respiration and thermoregulation, and sensory/perceptual changes, including echolocation capabilities and diminished olfaction in many cetaceans, both in-air and underwater visual focus, and enhanced senses of touch in pinnipeds and sirenians. Having a terrestrial foundation on which aquatic capacities were overlaid likely affected their cognitive abilities, especially as a new reliance on sound and touch, and the need to surface to breath changed their interactions with the world. Vocal and behavioral observational learning capabilities in the wild and in laboratory experiments suggest versatility in group coordination. Empirical reports on aspects of intelligent behavior like problem-solving, spatial learning, and concept learning by various species of cetaceans and pinnipeds suggest rich cognitive abilities. The high energy demands of the brain suggest that brain-intelligence relationships might be fruitful areas for study when specific hypotheses are considered, e.g., brain mapping indicates hypertrophy of specific sensory areas in marine mammals. Modern neuroimaging techniques provide ways to study neural connectivity, and the patterns of connections between sensory, motor, and other cortical regions provide a biological framework for exploring how animals represent and flexibly use information in navigating and learning about their environment. At this stage of marine mammal research, it would still be prudent to follow Macphail’s caution that it is premature to make strong comparative statements without more empirical evidence, but an approach that includes learning more about how animals flexibly link information across multiple representations could be a productive way of comparing species by allowing them to use their specific strengths within comparative tasks.

Summary Introduction Relative Energy Deficiency in Sport (RED-S) is a clinical syndrome that includes the many complex health and performance consequences of low energy availability (EA) in athletes, when there is insufficient caloric intake to meet exercise-related energy expenditure and to support basic physiologic functions. There is a high prevalence of urinary incontinence (UI) in female athletes and it is more common in female athletes than non-athletes. The objective of this study was to determine if low EA is associated with UI in a population of adolescent and young adult female athletes and to evaluate for an association between sport categories and UI. Material and methods 1000 nulliparous female patients, ages 15–30 years, presenting to a sports medicine subspecialty clinic, provided informed consent/assent to participate in a cross-sectional study involving a comprehensive survey, anthropomorphic measurements, and medical record review. Low EA was defined as meeting ≥1 criterion: self-reported history of eating disorder/disordered eating (ED/DE), and/or a high score on the Brief Eating Disorder in Athletes Questionnaire (BEDA-Q), and/or a high score on the Eating Disorder Screen for Primary Care (ESP). UI was assessed using questions adapted from the International Consultation on Incontinence-Urinary Incontinence Short Form (ICIQ-UI-SF), questions regarding timing of UI onset/duration, and a binary question regarding UI during sport activities. A total of 36 sport types were included in the survey and sub-divided into categories. Results and discussion Of the 1000 female athletes surveyed, 165 (16.5%) reported a history of experiencing UI during athletic activities. ICIQ- UI-SF responses indicated that 14% (137/1000) of the cohort experienced slight incontinence, 4% (35/1000) moderate incontinence, and 2 athletes experienced severe incontinence. There was a significant difference between UI categories in age (p = 0.01), low EA (p Conclusions UI during athletic activities is a common problem among nulliparous adolescent and young adult female athletes, occurring in 16.5% of female athletes surveyed. UI was significantly associated with low EA across all sport categories. Sport type was significantly associated with UI, with the highest impact sport group demonstrating a higher prevalence and symptom severity compared to other sport categories. Summary Table . Characteristics of Female Athletes with Urinary Incontinence Entire cohort N = 1000 Athletes with Urinary incontinence n = 165 (16.5%) P-value Freq. (%) Freq. (%) Low energy availability 473 (47%) 99 (20.9%) Menstrual dysfunction 449 (45%) 80 (17.8%) 0.31 Sport category: High impact 49 (5%) 18 (36.7%) Ball/field 356 (36%) 46 (12.9%) Non-weightbearing endurance 60 (6%) 6 (10.0%) Aesthetic 173 (17%) 25 (14.5%) Resistance/power 106 (11%) 23 (21.7%) Running 222 (22%) 44 (19.8%) Technical 34 (3%) 3 (8.8%)

PURPOSE: Congenital anomalies of the kidney and urinary tract (CAKUT) are the most common cause of chronic kidney disease in childhood and adolescence. We aim to identify novel monogenic causes of CAKUT. METHODS: Exome sequencing was performed in 550 CAKUT-affected families. RESULTS: We discovered seven FOXC1 heterozygous likely pathogenic variants within eight CAKUT families. These variants are either never reported, or present in

The synthesis and characterization of a novel macrocyclic Au(III) N-heterocyclic carbene (NHC) imidazolyl complex, a novel macrocyclic tetra-NHC benzimidazole ligand, and the corresponding Ag(I) and Au(I) complexes are presented. Single-crystal X-ray diffraction analysis of the Au(I) benzimidazolyl complex 3 reveals an unusual structure, differing from the respective Au(I) imidazolyl complex 4. Both complexes have a Au4L2 composition; however, 3 has two C–Au(I)–C units acting as a connection between the two ligands with two Au(I) atoms being linearly coordinated inside the cavity of the macrocyclic ligand. In the case of complex 4, the structure shows a box-type coordination with all four Au(I) atoms being located between the two ligands. Stability studies in cell culture medium are performed for subsequent MTT assays and they show an unprecedented proton-to-deuterium exchange of the methylene bridge of the Au(III) imidazolyl complex. In MTT assays, the tetranuclear acyclic Au(I) complex 5 displays the lowest IC50 values in MCF-7, PC3, and A2780cisR cells with a selective cytotoxicity for MCF-7 and A2780cisR cells.

Human studies have shown loss of diversity of the gut microbiome following hematopoietic stem cell transplantation (HSCT) in association with significant gut injury caused by the preparative regimen. Prolonged antibiotic use worsens loss of microbiome diversity and increases risk of complications such as graft-versus-host disease (GVHD). Our data support the hypothesis that loss of intestinal commensals that produce short-chain fatty acids (SCFAs) may increase dysbiosis. Here, we report an extensive longitudinal examination of changes in the luminal SCFAs in children undergoing allogeneic HSCT, and the relationship of those changes to the microbiota and antibiotic exposure. We found significant and progressive alterations in butyrate, and in additional SCFAs in stool in the first 14 days after transplant, a finding not observed in published mouse studies. SCFA levels were lower in children receiving antibiotics with activity against anaerobic organisms. Moreover, day 14 post-HSCT butyrate and propionate levels are lower in children who went on to develop GVHD, although our disease population was small. These data provide insight into the mechanism of prior observations that loss of diversity and increased antibiotic use are associated with GVHD following HSCT. Our findings offer potential modifiable targets to reduce risk of GVHD and improve survival after HSCT.

Aims: To introduce the standard procedure of cystometry and interpretation of the results in children. Methods: The literature on cystometry in children in PubMed for the last 20 years was reviewed. The updated knowledge regarding indication, preparation, technique, and interpretation of cystometry in children were summarized. Results: Filling cystometry is the core content of a paediatric urodynamic study. In this section, the technique for performing cystometry is introduced in details. Emphasis is placed on correctly setting up the equipment according to ICS and ICCS guidelines, using appropriate terminology, providing indications for its performance with specific considerations for children, and proper interpretation of results. Conclusions: Cystometry can be used in children including newborn to evaluate lower urinary tract dysfunction.

After activation by chloride abstraction utilizing NaBArF as an activator (BArF = tetrakis(3,5-bis(trifluoromethyl)phenyl)borate), a complex of the general formula [RuCl2(PHOX)2] was utilized as a catalyst in propargylic substitution reactions, where PHOX is a phosphinooxazoline ligand. Oxygen and nitrogen-centered nucleophiles could be employed in the substitution of a propargylic acetate to obtain the corresponding propargylic substitution products in 87% to 9% isolated yields (45 °C, 16 h reaction time, toluene solvent, 1–2 mol% catalyst loading, 1–2 mol% activator).

A new ruthenium 2,6-diacetylpyridine complex was synthesized and applied in the atom-economic synthesis of enol esters through Markovnikov-directed addition of carboxylic acids to terminal alkynes. The ruthenium complex [RuCl(dap)(PPh3)2]+BArF− was synthesized from [RuCl2(PPh3)2] and the corresponding ligand 2,6-diacetylpyridine (dap). The complex was characterized structurally. The new ruthenium complex was utilized under ambient conditions as a catalyst in the Markovnikov addition of carboxylic acids to terminal alkynes to afford the corresponding enol esters in 93% to 52% isolated yields (85 °C, 16 h reaction time, 1 mol% catalyst loading).

To investigate the correlation between urethral instability (URI) and overactive bladder (OAB) in children. We retrospectively investigated 126 children with OAB and 36 children without OAB using synchro-cystourethrometry. The prevalence of detrusor overactivity (DO) and URI, and the diagnostic sensitivity of DO alone and DO combined with URI, was compared. The OAB children with URI voluntarily received transcutaneous electrical pudendal nerve stimulation with anisodamine (stimulation group, SG) or anisodamine alone (non-stimulation group, NSG). The effectiveness of treatment was evaluated. Average voided volume (AVV), maximum voided volume (MVV), and number of voids per day (NV) were collected and analyzed. In OAB children, the prevalence of DO and URI was 51.6 and 32.5%, respectively. The prevalence of URI was 5.6% in controls. The prevalence of URI was significantly higher in OAB children. The diagnostic sensitivity and Youden index of DO combined with URI were higher than DO alone. In SG, 45.7% of children were cured, with a ≥ 50% improvement rate of 82.9%, while no child was cured, with a ≥ 50% improvement rate of 36.8% in NSG. A significant increase in AVV and MVV together, with a decrease in NV, was seen in SG. There was a significant difference in visual analogue scale values between SG and NSG (P

Item does not contain fulltext BACKGROUND: Congenital anomalies of the kidney and urinary tract (CAKUT) are the most prevalent cause of kidney disease in the first three decades of life. Previous gene panel studies showed monogenic causation in up to 12% of patients with CAKUT. METHODS: We applied whole-exome sequencing to analyze the genotypes of individuals from 232 families with CAKUT, evaluating for mutations in single genes known to cause human CAKUT and genes known to cause CAKUT in mice. In consanguineous or multiplex families, we additionally performed a search for novel monogenic causes of CAKUT. RESULTS: In 29 families (13%), we detected a causative mutation in a known gene for isolated or syndromic CAKUT that sufficiently explained the patient's CAKUT phenotype. In three families (1%), we detected a mutation in a gene reported to cause a phenocopy of CAKUT. In 15 of 155 families with isolated CAKUT, we detected deleterious mutations in syndromic CAKUT genes. Our additional search for novel monogenic causes of CAKUT in consanguineous and multiplex families revealed a potential single, novel monogenic CAKUT gene in 19 of 232 families (8%). CONCLUSIONS: We identified monogenic mutations in a known human CAKUT gene or CAKUT phenocopy gene as the cause of disease in 14% of the CAKUT families in this study. Whole-exome sequencing provides an etiologic diagnosis in a high fraction of patients with CAKUT and will provide a new basis for the mechanistic understanding of CAKUT. 01 september 2018

BACKGROUND: We present a consensus view from the International Children's Continence Society (ICCS) on the evaluation and management of bladder bowel dysfunction (BBD) in children with urinary tract infection (UTI). The statement aims to highlight the importance of BBD in the development and recurrence of childhood UTI and its management to reduce its associated morbidity and sequelae.METHODS: A systematic literature search was done on PubMed, Embase, and Scopus databases until August 15, 2016. Relevant publications concerning BBD and its relationship with UTI among children were reviewed and aggregated for statements of recommendation. Discussion by the ICCS Board and a multi-disciplinary core group of authors resulted in a document available on its website for all ICCS members to review. Insights and feedback were considered with consensus and agreement reached to finalize this position statement.RESULTS: BBD in children with UTI is summarized. Details regarding epidemiology, pathophysiology, and recommendations for general and family practitioners and pediatricians relating to the evaluation and management of this condition are presented.CONCLUSIONS: This document serves as the position statement from ICCS, based on literature review and expert opinion providing our current understanding of BBD in children with UTI.

The synthesis, characterization and catalytic activity of new ruthenium complexes of fluorinated triarylphosphines is described. The new ruthenium complexes [RuCl(ind)(PPh3){P(p-C6H4CF3)3}] and [RuCl(ind)(PPh3){P(3,5-C6H3(CF3)2)3}] were synthesized in 57% and 24% isolated yield, respectively, by thermal ligand exchange of [RuCl(ind)(PPh3)2], where ind = indenyl ligand η5-C9H7−. The electronic and steric properties of the new complexes were studied through analysis of the X-ray structures and through cyclic voltammetry. The new complexes [RuCl(ind)(PPh3){P(p-C6H4CF3)3}] and [RuCl(ind)(PPh3){P(3,5-C6H3(CF3)2)3}] and the known complex [RuCl(ind)(PPh3)2}] differed only slightly in their steric properties, as seen from comparison of bond lengths and angles associated with the ruthenium center. As determined by cyclic voltammetry, the redox potentials of [RuCl(ind)(PPh3){P(p-C6H4CF3)3}] and [RuCl(ind)(PPh3){P(3,5-C6H3(CF3)2)3}] are +0.173 and + 0.370 V vs. Cp2Fe0/+, respectively, which are substantially higher than that of [RuCl(ind)(PPh3)2] (−0.023 V). After activation through chloride abstraction, the new complexes are catalytically active in the etherification of propargylic alcohols (8–24 h at 90 °C in toluene, 1–2 mol% catalyst loading, 29–61% isolated yields). As demonstrated by a comparative study for a test reaction, the three precursor complexes [RuCl(ind)(PPh3){P(p-C6H4CF3)3}], [RuCl(ind)(PPh3){P(3,5-C6H3(CF3)2)3}] and [RuCl(ind)(PPh3)2}] differed only slightly in catalytic activity.

The near constant use of technology today has led to widespread changes in the way literacy is imagined, used, and theorized. Since college students spend a significant amount of time using and bei...