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222 results on '"Benini E"'

53. Educazione e generazioni

Author

Barbato, B, Benini, E, Beambilla, L, De Leo, A, Micelli, M, Pozzo, M, Rizzo M, Tramma, S, Brambilla, B, Benini, A, BENINI, ANNALISA, POZZO, MATILDE MAIA, Barbato, B, Benini, E, Beambilla, L, De Leo, A, Micelli, M, Pozzo, M, Rizzo M, Tramma, S, Brambilla, B, Benini, A, BENINI, ANNALISA, and POZZO, MATILDE MAIA

Published
2014

62. The Giada Experiment for the Rosetta Mission

Author

Palumbo, P., Palomba, E., Bussoletti, E., Colangeli, L., Epifani, E., Vito Mennella, Rotundi, A., Vergara, S., Fulle, M., Angrilli, F., Benini, E., Celli, S., Debei, S., and Saggin, B.

Published
1997

65. Synthesis, Characterization, and Biological Evaluation of Neutral Nitrido Technetium(V) Mixed Ligand Complexes Containing Dithiolates and Aminodiphosphines. A Novel System for Linking Technetium to Biomolecules

Author

Bolzati, C., Benini, E., Cazzola, E., Jung, C., Tisato, F., Refosco, F., Pietzsch, H.-J., Spies, H., Uccelli, L., Duatti, A., Bolzati, C., Benini, E., Cazzola, E., Jung, C., Tisato, F., Refosco, F., Pietzsch, H.-J., Spies, H., Uccelli, L., and Duatti, A.

Abstract

A new biomolecule labeling method that utilizes the [99mTc(N)(PNP)]2+m metal fragment is presented. Thus, a series of nitrido mixed-ligand M(V) complexes (M = 99mTc, 99gTc, Re), [M(N)(Ln)(PNP)], where Ln is the dianionic form of a dithiolate or substituted-dithiolate ligand and PNP is an aminodiphosphine, is described. 99mTc complexes can be prepared using either a two-step or a three-step procedure starting from generator-eluted pertechnetate through a pre-reduced mixture of [99mTc(N)] containing species, followed by contemporary or sequential addition of the relevant dithiolate and aminodiphosphine. The reaction of 2,3-dimercapto propionic acid (H2L1) with [Tc(N)(PNP)]2+ was investigated in detail. It was found that this bidentate ligand coordinated the metal fragment through the [S-,S-] donor atom pair, to yield neutral mixed-ligand complexes [99mTc(N)(L1)(PNP)] in high specific activity. The additional carboxylic functional group was not involved in metal coordination, thus remaining available for conjugation to target-specific molecules. Dithiolates incorporating pendant functional group(s) gave rise to a 1:1 diastereoisomeric mixture of syn-[M(N)(Ln)(PNP)] and anti-[M(N)(Ln)(PNP)] derivatives, depending on the relative orientation of the dithiolate substituent(s) with respect to the terminal nitrido group, and no isomeric conversion was detected. 99mTc species had been proven to be identical with the 99gTc complexes prepared at the macroscopic level by comparison of the corresponding radiometric and UV/vis HPLC profiles. Challenge experiments with cysteine or glutathione indicated that these physiological agents had no effect on the stability of this class of mixed-ligand 99mTc-complexes. Biodistribution studies in rats of selected 99mTc-complexes showed a rapid clearance from the blood and tissues

Published
2004

72. Expression of p53, glutathione S-transferase-pi, and Bcl-2 proteins and benefit from adjuvant radiotherapy in breast cancer.

Author

Silvestrini R, Veneroni S, Benini E, Daidone MG, Luisi A, Leutner M, Maucione A, Kenda R, Zucali R, Veronesi U, Silvestrini, R, Veneroni, S, Benini, E, Daidone, M G, Luisi, A, Leutner, M, Maucione, A, Kenda, R, Zucali, R, and Veronesi, U

Abstract

Background: In clinical breast cancer research, the utility of certain biomarkers as predictors of response to surgery, chemotherapy, or hormonal therapy has been studied intensively. Much less research has been done on the relevance of biologic predictors of response to radiotherapy, which represents an effective local-regional treatment for breast cancer.Purpose: The utility of biomarkers involved in DNA damage repair (p53 protein), control of programmed cell death (p53 and Bcl-2 proteins), and cellular detoxification (glutathione S-transferase-pi [GST-pi] enzyme) in predicting local breast cancer recurrence was analyzed retrospectively in two cohorts of breast cancer patients. These patients had had no detectable metastases in the axillary lymph nodes (i.e., node-negative) or in distant sites and had had similar distributions of clinicopathologic and biologic prognostic features. They had been treated by conservative surgery alone (139 case patients) or by conservative surgery followed by adjuvant radiotherapy (496 case patients) during the period from 1984 through 1990.Methods: The expression of the p53, GST-pi, and Bcl-2 proteins in the specimens of primary breast tumor obtained from these patients was determined by use of immunohistochemistry; cell proliferation activity and levels of steroid receptors were determined by use of a [3H]thymidine-labeling index assay and the dextran-coated charcoal technique, respectively. The median time of follow-up of patients was 6 years. In the analyses of patient outcomes, only local failures that presented as first events were considered.Results: After surgery alone, the risk of local recurrence at 6 years was higher for patients with tumors exhibiting elevated levels of p53 and GST-pi protein expression than for patients with low levels (hazard ratio [HR] = 3.1, 95% confidence interval [CI] = 1.3-7.7, two-sided P = .012; HR = 2.7, 95% CI = 1.1-6.4, two-sided P = .026, respectively). Weak or no observable expression of Bcl-2 protein was only suggestive of a higher frequency of local failures. Adjustment for patient age, tumor size, cell proliferation, and estrogen receptor status did not change these findings. Conversely, in the series of patients given conservative surgery followed by radiotherapy, there was no difference in local tumor recurrence between patients with tumors expressing or not expressing each of the three markers.Conclusions: Our study provides indirect evidence of a benefit from radiation therapy in preventing local breast cancer relapse, particularly among node-negative patients with tumors that express elevated levels of the p53 or GST-pi proteins or that express little or no Bcl-2 protein. [ABSTRACT FROM AUTHOR]

Published
1997
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89. A Novel Approach to the High-Specific-Activity Labeling of Small Peptides with the Technetium-99m Fragment [<SUP>99m</SUP>Tc(N)(PXP)]<SUP>2+</SUP> (PXP = Diphosphine Ligand)

Author

Boschi, A., Bolzati, C., Benini, E., Malago, E., Uccelli, L., Duatti, A., Piffanelli, A., Refosco, F., and Tisato, F.

Abstract

A new labeling approach for incorporating bioactive peptides into a technetium-99m coordination complex is described. This method exploits the chemical properties of the novel metal-nitrido fragment [99mTc(N)(PXP)]2+, composed of a terminal Tc&tbd1;N multiple bond bound to an ancillary diphosphine ligand (PXP). It will be shown that this basic, molecular building block easily forms in solution as the dichloride derivative [99mTc(N)(PXP)Cl2], and that this latter complex selectively reacts with monoanionic and dianionic, bidentate ligands (YZ) having soft, π-donor coordinating atoms to afford asymmetrical nitrido heterocomplexes of the type [99mTc(N)(PXP)(YZ)]0/+ without removal of the basic motif [99mTc(N)(PXP)]2+. The reactions of the amino acid cysteine was studied in detail. It was found that cysteine readily coordinates to the metal fragment [99mTc(N)(PXP)]2+ either through the [NH2, S-] pair of donor atoms or, alternatively, through the [O-, S-] pair, to yield the corresponding asymmetrical complexes in very high specific activity. Thus, these results were conveniently employed to devise a new, efficient procedure for labeling short peptide sequences having a terminal cysteine group available for coordination to the [99mTc(N)(PXP)]2+ fragment. Examples of the application of this novel approach to the labeling of the short peptide ligand H-Arg-Gly-Asp-Cys-OH (H21) and of the peptidomimetic derivative H-Cys-Val-2-Nal-Met-OH (H2) will be discussed.

Published
2001

94. ERICA tiltrotor airframe wake characterization

Author

Fabrizio De Gregorio, Steiling, D., Benini, E., and Ponza, R.

Subjects
WT test campaign, Drag reduction, Tiltrotor, Wake characterization, Aerodynamic optimization, CFD, S-PIV

95. The GIADA experiment for Rosetta mission to comet 46P/Wirtanen: Design and performances

Author

Bussoletti, E., Colangeli, L., Lopez Moreno, J. J., Epifani, E., Mennella, V., Palomba, E., Palumbo, P., Rotundi, A., Vergara, S., Girela, F., Herranz, M., Jeronimo, J. M., Lopez-Jimenez, A. C., Molina, A., Moreno, F., Olivares, I., Rodrigo, R., Julio F. Rodríguez-Gómez, Sanchez, J., Mc Donnell, J. A. M., Leese, M., Lamy, P., Perruchot, S., Crifo, J. F., Fulle, M., Perrin, J. M., Angrilli, F., Benini, E., Casini, L., Cherubini, G., Coradini, A., Giovane, F., Grün, E., Gustafson, B., Maag, C., and Weissmann, P. R.

Subjects
Space and Planetary Science, Aerospace Engineering

98. Quantitative YAP-(S)PET small animal scanner: preliminary results

Author

Di Domenico, G., primary, Zavattini, G., additional, Moretti, E., additional, Piffanelli, A., additional, Giganti, M., additional, Motta, A., additional, Sabba, N., additional, Uccelli, L., additional, Benini, E., additional, Duatti, A., additional, Bolzati, C., additional, Boschi, A., additional, and Del Guerra, A., additional

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