51. Integrated analysis of chromosome copy number variation and gene expression in cervical carcinoma
- Author
-
Tang Tao, Chung Tony Kwok Hung, Choy Kwong Wai, Song Yi, Wong Hoi Kin, Deng Yan, Liu Gui Qin, Yang Tao, Han Linxiao, Sui Yi, and Wang Chi Chiu
- Subjects
0301 basic medicine ,Oncology ,medicine.medical_specialty ,Microarray ,cervical cancer ,Biology ,03 medical and health sciences ,0302 clinical medicine ,chromosome copy number variation ,Internal medicine ,Gene expression ,Cervical carcinoma ,medicine ,Copy-number variation ,Gene ,Cervical cancer ,Genetics ,cell cycle pathways ,Chromosome ,medicine.disease ,030104 developmental biology ,030220 oncology & carcinogenesis ,gene expression ,Human genome ,Research Paper ,cluster analysis - Abstract
// Deng Yan 1, 2, * , Song Yi 1, * , Wang Chi Chiu 1, 2 , Liu Gui Qin 3 , Wong Hoi Kin 1 , Chung Tony Kwok Hung 1 , Han Linxiao 4 , Choy Kwong Wai 1, 2 , Sui Yi 5 , Yang Tao 6 and Tang Tao 1, 2 1 Department of Obstetrics & Gynaecology, The Chinese University of Hong Kong, Hong Kong, China 2 CUHK Shenzhen Research Institute, Shenzhen, China 3 Shenzhen Laboratory of Ophthalmology, Shenzhen Eye Hospital, Affiliated Shenzhen Eye Hospital of Shenzhen University, Shenzhen, China 4 Dongguan Third People’s Hospital, Dongguan, China 5 Department of Nutrition, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China 6 Center for Medical Research and Innovation, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, China * These authors have contributed equally to this work Correspondence to: Tang Tao, email: tangtao@cuhk.edu.hk Yang Tao, email: mryangtao2005@yahoo.com Sui Yi, email: suiyisy@hotmail.com Keywords: cervical cancer; chromosome copy number variation; gene expression; cluster analysis; cell cycle pathways Received: April 19, 2017 Accepted: September 21, 2017 Published: November 11, 2017 ABSTRACT Objective: This study was conducted to explore chromosomal copy number variations (CNV) and transcript expression and to examine pathways in cervical pathogenesis using genome-wide high resolution microarrays. Methods: Genome-wide chromosomal CNVs were investigated in 6 cervical cancer cell lines by Human Genome CGH Microarray Kit (4x44K). Gene expression profiles in cervical cancer cell lines, primary cervical carcinoma and normal cervical epithelium tissues were also studied using the Whole Human Genome Microarray Kit (4x44K). Results: Fifty common chromosomal CNVs were identified in the cervical cancer cell lines. Correlation analysis revealed that gene up-regulation or down-regulation is significantly correlated with genomic amplification ( P =0.009) or deletion ( P =0.006) events. Expression profiles were identified through cluster analysis. Gene annotation analysis pinpointed cell cycle pathways was significantly ( P =1.15E-08) affected in cervical cancer. Common CNVs were associated with cervical cancer. Conclusion: Chromosomal CNVs may contribute to their transcript expression in cervical cancer.
- Published
- 2017