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Introduction: The recent development of extensively antibiotic resistant bacteria has necessitated the search for novel antibacterial therapies. An examination of aromatic plants and traditional medicines is an attractive option for drug discovery. Terminalia sericea Burch. ex DC. is a southern African native species, which has been reported to have antibacterial activity, although the effects of storage on the activity is yet to be determined. Materials and Methods: The ability of T. sericea leaf extracts to inhibit the growth of a panel of bacterial triggers of autoimmune diseases was quantified by disc diffusion and liquid dilution MIC assays. The potency of extracts prepared using fresh and stored leaves was compared. Toxicity was examined using the Artemia franciscana nauplii bioassay. Results: The T. sericea leaf methanolic and aqueous extracts displayed noteworthy growth inhibitory activity towards all bacterial pathogens tested. However, a substantial decrease in activity was seen when extracts were prepared using leaves that had been stored for two years, indicating that the leaves become less useful therapeutically over time. All extracts were non-toxic in the Artemia nauplii bioassay following 24hr exposure. Conclusion: The T. sericea leaf extracts had noteworthy antibacterial activity, although the potency decreased substantially following storage of the leaf material. [ABSTRACT FROM AUTHOR]

Introduction: Terminalia arjuna (Roxb.) Wight and Arn. is a southern Asian plant commonly used in Ayurveda to treat multiple conditions, including for the treatment of inflammation, and as a general antimicrobial agent. Despite this, few studies have tested this species for the ability to block the growth of bacterial triggers of some autoimmune inflammatory diseases. Furthermore, the ability of T. arjuna to potentiate the activity of conventional antibiotics is yet to be investigated. Materials and Methods: The minimum inhibitory concentration of the extracts and extract-antibiotic combinations was determined by disc diffusion and liquid dilution MIC methods. Fractional inhibitory concentration values were calculated to evaluate the combinational effect of combinations of extracts and conventional antibiotics. When synergistic interactions were detected, isobologram analysis was used to determine the ideal ratios for synergy. Toxicity was evaluated by Artemia nauplii mortality and HDF cell viability assays. Results: Methanolc, aqueous and ethyl acetate T. arjuna extracts were good inhibitors of P. mirabilis and K. pneumoniae growth when tested alone. The methanolic and aqueous extracts also synergised the inhibitory activity of ciprofloxacin against P. mirabilis and K. pneumoniae when used in combination. Additionally, numerous additive combinational effects were noted. T. arjuna chloroform extract was also a moderate inhibitor of a multi-drug resistant P. aeruginosa strain when tested alone, but did not potentiate the activity of any of the conventional antibiotics tested. All extracts and extract-antibiotic combinations were nontoxic in the Artemia nauplii mortality and HDF proliferation assays, indicating their suitability for therapeutic use. Conclusion: T. arjuna extracts have potential as inhibitors of bacterial triggers of selected autoimmune inflammatory diseases. Furthermore, T. arjuna extracts potentiate the activity of ciprofloxacin against P. mirabilis and K. pneumoniae and therefore may be beneficial in drug design against these bacteria. [ABSTRACT FROM AUTHOR]