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Objectives. To assess changes in the spectral characteristics of the electroencephalogram (EEG) in humans during single sessions of biocontrol of hart rate variability parameters using biological feedback (BFB). Materials and Methods. A total of 150 adolescents aged 16–17 years were studied. Adolescents of the BFB group (n = 110) took part in biocontrol sessions to increase vagal influences on heart rate; the control group (n = 40) remained in the state of calm wakefulness. EEG recordings were made with the eyes closed before and after BFB training. EEG characteristics were studied in terms of changes in absolute power levels (μV2) in artifact-free trace segments in each frequency range. Results and conclusions. The ability of human subjects to change the activity of heart rate parameters determines the level of action on the functions of the central autonomic regulatory structures. This biocontrol method promotes improvements in cortical stability, with increases in α and decreases in β EEG activity. Brain activity synchronization processes during biocontrol were more clearly apparent in the right hemisphere, often involving the prefrontal areas.

An approximate approach based on application of the pattern equation method is developed for solution of the problem of scattering by a thin cylinder. Explicit formulas for integral scattering characteristics that are applicable to cylindrical scatterers of a complex shape are derived. The accuracy of these formulas is controlled by checking fulfillment of the “optical theorem.”

Pituitary tumor-transforming gene-1 (PTTG1) encodes securin, a multifunctional protein involved in development of various types of cancer. Securin participates in the regulation of sister chromatids separation and the expression of multiple genes involved in the control of the cell cycle, metabolism, and angiogenesis. In several human cell lines, we have found a novel short isoform of securin mRNA, which does not contain exons 3 and 4. After the translation of this new mRNA, a shortened protein is produced that, like the full-size form, is able to activate the transcription of cyclin D3 gene (CCND3), which controls the G1/S transition and angiogenesis factors VEGFA (vascular endothelial growth factor), and FGF2 (fibroblast growth factor 2) in HEK293 cells. However, unlike the full-size protein, the short isoform of PTTG1 does not affect the MYC gene expression because it lacks the DNA-binding domain, which is needed for its interactions with the MYC promoter. Furthermore, the short form of securin does not influence the expression of MYC transcriptional targets, such as TP53 and IL-8. Thus, we found a novel isoform of securin which is able to activate a more restricted repertoire of genes compared to the full-size protein.

During the works by calculation, there were grounded the makeup of fuel briquettes, which are produced from oil wastes, coal and biomass - rice husk. Within changing considered makeups and concentration, combustion heat values calculated, which in future will allow to compare the experimental values of the fuel characteristics of the briquettes with an assigned structural composition

The paper summarizes the experience of studying the effect of modern warming on the thawing depth of sandy and loamy soils in the permafrost zone. The definition of soil cryogenesis as a natural process and facies as the end result of soil cryogenesis as a process is given. The effect of soil cryogenesis facies on stress corrosion cracking of gas pipes of the Gazprom system in cold and warm seasons has been studied. It was found that the facies of soil cryogenesis are interconnected by the process of energy and mass exchange. It is shown that in the annual cycle, each facies formed by soil cryogenesis is the basis for the development of stress corrosion cracking of gas pipelines.

Oil production, transportation and refining are a source of the extensive environmental pollution. The study of oil pollutants allows the identification of their behavior pattern necessary in order to elaborate efficient decontamination technologies. The Absheron oil field is one of the most long-term exploited deposit in the world. Today this area is a unique place to investigate the long-lasting natural oil transformation processes. Grey-Brown Soils contaminated with crude oil 100 and 40 years ago and currently were investigated. Hexane-extractable fraction of oil compounds was examined by chemical methods, gas chromatography and mass spectrometry. In the process of natural degradation, molecular weight of oil hydrocarbons and degree of oxidation significantly increased, while the contents of carbon and hydrogen slightly decreased with time. The results obtained have shown the accumulation of high-molecular weight aromatic molecules with high degree of unsaturation in the upper layer of the soil contaminated 100 years ago. These aromatic structures exhibit the great resistance to decomposition and may be carcinogenic, thus posing a risk to human health. The contamination with oil resulted in altering soil properties. More pronounced change was observed in the soil carbon, including the soil contaminated 100 years ago. Over time after contamination, the soil alkalinity enhanced, whereas soil pH did not change. Our study evidences that oil pollutants continue to be present and the soil properties have not recovered even 100 years after pollution. Reclamation of these areas requires the application of special technologies.

In the first part of the review the historical aspect of scientific fields of age neurophysiology has been provided, age-related features of morphologic and functional development of the brain and their reflection in his bioelectric activity have been considered. It has been shown that the morphological maturation of different brain structures reflected both in the psycho-physiological development of the child and in changes of amplitude-frequency spectrum of electroencephalogram and in the spatio-temporal organization of brain potentials. Literature data describing the developmental of electroencephalogram dynamics in children and adolescents have been presented. Age formation stages of electrogenesis of different areas of the brain, as well as specific changes in intra- and interhemispheric relationships have been shown. In the second part of the review peculiarities of the autonomic nervous system functioning in childhood and adolescence have been discussed; emphasis has been placed on the reactions of the cardiovascular system in patients with different types of vegetative tonus. These studies have demonstrated the changes of central hemodynamic and heart rate variability in adolescents according to age, sex, and living conditions. In addition, the main factors of external and internal environment that could cause the development of neurologic disorders and vegetative dysfunctions in children and adolescents have been considered.

The MAPK (RAS/BRAF/MEK/ERK) signaling pathway is a kinase cascade involved in the regulation of cell proliferation, differentiation, and survival in response to external stimuli. The V600E mutation in the BRAF gene has been detected in various tumors, resulting in a 500-fold increase in BRAF kinase activity. However, monotherapy with selective BRAF V600E inhibitors often leads to reactivation of MAPK signaling cascade and emergence of drug resistance. Therefore, new targets are being developed for the inhibition of components of the aberrantly activated cascade. It was recently discovered that resistance to BRAF V600E inhibitors may be associated with the activity of the tyrosine phosphatase SHP-2 encoded by the PTPN11 gene. In this paper, we analyzed transcriptional effects of PTPN11 gene knockdown and selective suppression of BRAF V600E in a model of thyroid follicular epithelium. We found that the siRNA-mediated knockdown of PTPN11 after vemurafenib treatment prevented an increase in the expression CCNA1 and NOTCH4 genes involved in the formation of drug resistance of tumors. On the other hand, downregulation of PTPN11 expression blocked the transcriptional activation of genes (p21, p15, p16, RB1, and IGFBP7) involved in cell cycle regulation and oncogene-induced senescence in response to BRAF V600E expression. Therefore, it can be assumed that SHP-2 participates not only in emergence of drug resistance in cancer cells, but also in oncogene-induced cell senescence.

Sodium salts of amino acids in solution have an alkaline reaction, which makes it possible to effectively bind metals into complex compounds, since in an alkaline medium, under the action of hydroxyl ions, the dissociation of amine groups is suppressed, and carboxyl groups remain dissociated, charging negatively, and behave like an anion. In our experiments, we used an amino acid-based reagent. When processing various environments (objects) contaminated with heavy metals (HM) with this reagent, HM amino acid complexes are formed. In our experiments, we used an amino acid-based reagent. With processing various environments (objects) contaminated with heavy metals (HM) with this reagent are formed HM amino acid complexes. Model experiments to assess the effect of microorganisms under aerobic conditions on the stability of amino acid complexes of heavy metals. After biodegradation of solutions with TM complexes, no more than 2-3% of heavy metals pass through the filter, which is ten times less than from untreated with the reagent solution.

Incorrect reparation of DNA double-strand breaks (DSB) leading to chromosomal rearrangements is one of oncogenesis’s primary causes. Recently published data elucidate the key role of various types of RNA in DSB formation, recognition and repair. With growing interest in RNA biology, increasing RNAs are classified as crucial at the different stages of the main pathways of DSB repair in eukaryotic cells: nonhomologous end joining (NHEJ) and homology-directed repair (HDR). Gene mutations or variation in expression levels of such RNAs can lead to local DNA repair defects, increasing the chromosome aberration frequency. Moreover, it was demonstrated that some RNAs could stimulate long-range chromosomal rearrangements. In this review, we discuss recent evidence demonstrating the role of various RNAs in DSB formation and repair. We also consider how RNA may mediate certain chromosomal rearrangements in a sequence-specific manner.

This review demonstrates the effectiveness of short-term HRV biofeedback sessions in an evaluation of human adaptation to uncomfortable environments. A single HRV biofeedback session can be used as a test for the effectiveness of cortical-visceral connections in patients with cardiovascular disease. In addition, this method can be used as short-term assistance in adaptation to social stressful factors.

To solve the problem related to scattering by a perfectly conducting thin cylinder, approximate approach was developed based on use of Pattern Equation Method (PEM). Explicit formulas were obtained for total scattering cross-section applicable for cylindric scatterers of complex shape. It is shown that calculation accuracy controlled by calculating the balance of power flows for incident and scattered waves (checking the accomplishment of optical theorem) is quite sufficient for practice.

In conditions of experimental acute pancreatitis of 60 guinea pigs and during the treatment of 89 patients with acute pancreatitis it was shown that the use of thioctic acid significantly reduces the manifestations of ischemic symptom , the severity of free radical processes and improves the patient's outcomes. The way for minimally invasive interventions under intraoperative ultrasound navigation consists in layered implementation of minimally invasive access of 2-3 cm in length, followed by the establishment of liquid aspiration from the wound channel of necrotic detritus, while emptying and drainaging the cavity under ultrasound control. The method allows one-step sanitation and drainage of cavity formations containing purulent dense necrotic tissue. This method is efficient, technically feasible in any surgical department, economically rational, since it does not require the purchase of additional surgical equipment. This complex of therapeutic measures allowed to reduce postoperative mortality in case of necrotizing pancreatitis to 9 % during the last three years.

This paper describes a method for direct measurement of the amplitude–frequency characteristics and the Q factor of empty superconducting niobium radio frequency Tesla-type cavities. An automated measuring complex that permits recording the superconductivity effect and measuring high Q values has been developed. Measurements have been made of the Q factors of the investigated objects (the first domestic 1.3-GHz niobium cavities) at a level no lower than 0.1·109 (with a maximum value of ~1.2·1010) and a level of relative losses lower than ~130 dB (with a minimum factor of ~139.7 dB) at liquid nitrogen temperature.

The character of the electroencephalogram (EEG) changes during a once procedure of heart rate variability biofeedback was considered in adolescents 14-17 years with different autonomic nervous types living in the different geographical latitudes and climatic, ecological conditions of the European North: the Subpolar (64°30' N) and Polar (67°40' N) areas. More intensive reduction of theta EEG activity (p < 0,05-0,001) with a predominance of the dynamics in the right brain hemisphere (p < 0,05), which continues to decline after the procedure occurs in adolescents of Polar region, especially in the group with a predominance of sympathetic influences on the heart activity. A common increasing of EEG alpha activity over all areas of the cerebral cortex (p < 0,05-0,001) with some displacement gradient in the front and center brain parts revealed in adolescents from all groups. A spectral power increment of beta1 EEG activity occur primarily at the expense of the front and right center-temporal brain parts (p < 0,05-0,001) in adolescents of both areas, but the biggest changes were found in persons with a balanced (normal) autonomic nervous tone (p < 0,05).

Improvement of forage quality is one of the essential issues to enhance livestock productivity. Bahiagrass is a popular fodder plant used as pasture species and for hay production, but it lacks the nutritional quality. In greenhouse experiment, the effect of silicon (Si) (calcium silicate and amorphous silicon dioxide) and nitrogen (ammonium nitrate) supplementation on bahiagrass growth and protein content was evaluated. Both types of fertilizers significantly increased the biomass and crude protein content in leaves of bahiagrass. Higher effect was obtained when Si was applied together with nitrogen. The increases were 78 to 100% for biomass and 57 to 59% for crude protein content. Considering that improved plant Si nutrition mitigates the negative effects of biotic and abiotic stresses, the Si involvement into fertilizer management strategy in fodder crops could be efficient for maintaining productivity and quality.

Performing comparison of EEG in adolescents living in the Northern and Southern regions and having different risk of Internet-addiction (IA).School adolescents of 16-17 years old, permanently living in the Northern (In the samples of Northern/Southern regions 25.5/38.2% individuals of group I; 63.6/47.3% - of group II; 10.9/14.5% - of group III were revealed. There was a tendency to increase EEG spectral power with increasing IA risk. In both regions the highest θ-band spectral power in the central zones and the left temporal zone were revealed among the representatives of group II (Thus, variants of adaptive reactions of the brain activity were revealed, which reflect specificity of the IA formation and heterochrony of the EEG pattern formation in adolescents of the Northern region.Провести сравнительную оценку электроэнцефалографических (ЭЭГ) особенностей у подростков, имеющих различный риск развития интернет-зависимости (ИЗ), с учетом их проживания в северном и южном регионах.Обследованы школьники в возрасте 16—17 лет, постоянно проживающие в северном (В выборках северного/южного регионов выявлено 25,5/38,2% лиц I группы; 63,6/47,3% — II группы; 10,9/14,5% — III группы. Отмечена тенденция к нарастанию мощности диапазонов ЭЭГ при увеличении риска ИЗ. В обоих регионах наиболее высокая мощность θ-диапазона выявлена у представителей II группы в центральных и левом височном отделах (Таким образом, выявлены варианты приспособительных реакций мозговой активности, отражающие особенности формирования ИЗ и гетерохронию возрастного становления паттерна ЭЭГ у подростков-северян.

The environmental concerns related to oil extraction, transportation and refining include the pollution with heavy metals (HMs). Currently, there is a growing interest in the study of an influence of silicon-rich materials, including such natural minerals as diatomite, zeolite, and others, on the HM behavior in the environment with regard to their exploitation in soil remediation strategy. In greenhouse pot experiment, the effects of natural zeolite on the biomass of barley plants grown in artificially cadmium (Cd)-contaminated soil and Cd uptake and translocation were studied. Zeolite applied to soil at a low rate (0.15%) mitigated the Cd phytotoxicity and provided the same plant biomass production as in uncontaminated soil without zeolite. Zeolite stimulated development of root system. The application of zeolite at a low rate enhanced the phytoextraction process–cleaning up the soil from HM at the early (booting) stage of plant development.

The work is devoted to the use of organomineral fertilizer (OMF) based on food waste for sowing three mixtures of perennial cereals and the reclamation of an illuvial-ferruginous podbur in the Far North. Under the conditions of a model experiment, it was found that when OMF is introduced in fractions of 25 and 50%, the physicochemical properties of the soil improve. There is an increase in the green mass of plants relative to the control by 2.3 times. Adding 50% of the presented fertilizer leads to inhibition of cereals. The mass of plantings at this dose of fertilizer approaches the figures appearing in the experiment with 25% OMF. The presented organic-mineral fertilizer based on food waste can be used for the rehabilitation of disturbed soils of the illuvial-ferruginous podbur as fertilizer.

Hydrocarbon soil pollution is widespread problem in many regions of the world, where oil is extracted and transported. Pollution with crude oil has numerous adverse impacts on the soil-microorganism-plant system. Although adding fertilizers has been shown to be essential to enhance remediation rate, excessive nutrients may exert toxicity for microorganisms and plants. Field test was conducted in the area previously subjected to several accidental oil spills in the central European Russia. The effects of fresh pig manure and/or conventional NPK fertilizers on plant growth, hydrocarbon content and activities of catalase, dehydrogenase, urease, sucrose, and phosphatase were evaluated depending on an application rate. Fertilizer application resulted in restoration of plant cover and reduction of hydrocarbons by 13 to 46%. Oil pollution reduced activities of catalase, dehydrogenase, sucrase and phosphatase and increased urease activity. Manure application was more effective in regard to plant growth, hydrocarbon removal and enzymatic activity in comparison with mineral fertilizers. The maximum positive effect on contaminated soil-microorganism-plant system was obtained under combined application of manure and NPK.

The experience of studying the problem of corrosive aggressiveness of soils and grounds in terms of improving the reliability of Gazprom’s pipeline systems operation is summarized and presented. Soil factors causing surface corrosion of gas pipelines in soil conditions are studied. Based on the obtained results, it was suggested to evaluate not only the corrosive aggressiveness of soils and the gas pipe lifetime, but also the corrosive resistance of pipe metal in corrosive environments. Special attention is paid to the stress corrosion cracking formation mechanism.

The results of a model experiment on the use of organomineral fertilizer (OMF) based on food waste for the sowing of three mixtures of perennial cereals on a sample of the soil type of the Far North: an illuvial -ferruginous podzol with the aim of developing a technology for the restoration of disturbed soil cover are presented. It was revealed that when OMF is added in the amount of 25 and 50%, the physicochemical properties of the soil improve; there is an increase in the green mass of plants relative to control by an average of 3.3 times. When 50% of this fertilizer is applied, signs of plant inhibition are observed on day 18, which indicates an overabundance of nutrients in the soil. The mass of plants at this concentration is close to the values obtained at 25% OMF content. Organomineral fertilizer based on food waste with the obtained characteristics can be used in the restoration of the illuvial-ferruginous podzol as a reclamation fertilizer. Keywords: organic-mineral fertilizer, perennial cereals, physicochemical properties of soils.

PTTG1 (vertebrate securin) is a separation inhibitor and regulates DNA repair and transcription. The protein is predominantly expressed in the second half of the S phase and at the G2 stage. With the onset of anaphase, securin is ubiquitinated by the APC/C complex and degraded rapidly. Increased expression of PTTG1 is associated with enhanced tumor cell growth and metastasis. Recently, we found a short securin isoform lacking the main APC/C recognition site (D-box) and the DNA-binding domain encoded by exons 3 and 4. The mRNA level of the short isoform in unsynchronized cells is 0.4-2% of the full-length one. We reported earlier on the ability of the short PTTG1 isoform to activate some of the genes controlled by the full-length protein. In this work, groups of genes, whose expression is altered by the action of the short and complete securin isoforms, were determined using RNA sequencing. Groups of genes whose mRNA levels are regulated by both protein isoforms and only one of the isoforms were identified. For a more detailed study of the effect of securin isoforms on the transcriptional program of cells, the NFYB gene, encoding the NF-Y transcription regulator subunit, was chosen. Our data showed that with overexpression of the short isoform, the level of NFYB mRNA decreased 2.4 ± 0.7 times, while the complete isoform did not significantly affect the expression of NFYB. 2.2-fold suppression of the short isoform of securin led to an increase in the expression of NFYB mRNA by 2.7 ± 0.3 times. Moreover, the mRNA expression of full-length securin increased by 2.7 ± 0.4 times. Since NFYB is associated with the PTTG1 promoter region, we suggest that the short isoform may be involved in regulation of the expression of the main isoform of securin by changing the level of this transcription factor. Since NFYB and PTTG1 are involved in the development of tumors and the formation of drug resistance, we assume that the short isoform of securin may play an important role in these processes. Thus, we showed the functional significance of the minor short isoform of securin.

Polychlorinated biphenyls (PCB) are persistent organic pollutants used worldwide up 1990s. Although their use has been heavily restricted, PCB can be found in contaminated soils and sediments. The interaction of polychlorobiphenyls (PCBs) in soils with an alkaline amino acid reagent (NaL) and humic acids was studied. According to IR spectroscopy, intermolecular interaction of PCBs with NaL reagent was revealed. Chromatography and IR spectroscopy revealed the transformation and removal of chlorine in the aromatic ring of PCB macromolecules under the influence of an amino acid reagent. In the experiment, from 32 to 77% of PCBs undergo soil degradation, depending on the concentration of the reagent and the frequency of its introduction into soil samples.

A method for producing growth stimulants using liquid-phase catalytic oxidation of rice waste was tested, with an assessment of the composition of the resulting solution, active silicon, and bioassay. Laboratory studies have shown that solutions from rice waste synthesized by liquid-phase catalytic oxidation are promising for the preparation of plant growth stimulants.

Described in the article is a clinical case report concerning symptomatic subocclusion of the internal carotid artery in a male patient with a rare variant of recurrent limb-shaking transitory ischaemic attacks (LS-TIA). The patient presented with a three-month history of episodes of involuntary jerky movements in his left hand. These episodes occurred invariably on assuming a vertical position. The findings of duplex scanning revealed subocclusion of the right internal carotid artery. The patient was subjected to stenting with the use of the system of proximal cerebral protection. The postoperative period was complicated by the development of cerebral hyperperfusion syndrome the risk of which in patients with LS-TIA is known to be elevated. Via telephone interviewing carried out at 1, 6 and 12 months after the intervention, the patient confirmed that the episodes of hand shaking did not recur. This case report highlights the importance of accurately assessing the clinical findings while selecting patients for carotid revascularization, since such ischaemic episodes are strongly suggestive of a severe lesion of the contralateral carotid artery. Once subocclusion is revealed, it is optimal to perform stenting with the use of a system of proximal protection, as a safer and more effective method of surgical revascularization in such conditions.

The aim of this study was to determine the amplitude-frequency characteristics of the electroencephalogram (EEG) and heart rate variability (HRV) in humans, depending on the initial serum TNF-α level at a single session of heart rate variability biofeedback (HRV BF) due to increase the total power of the HRV spectrum. Among hypertensive individuals (blood pressure 140-160/80-100 mm Hg) subgroups with an optimal serum TNF-α level (below than 75 quartile - 84,4 pg/ml) and with a high serum TNF-α level (more than 84,4 pg/ml) were selected. In individuals with an optimal serum level of TNF-α, an increase in the total HRV spectral power, a decrease in the stress index, systolic blood pressure and a decrease in the EEG power in the theta range in the frontal brain regions after HRV BF session were identified. In individuals with a high serum TNF-α level the effectiveness of HRV BF was minimal against the background of continuing sympathicotonia and high theta EEG-activity.

The purpose of the work is to evaluate the features of the organization of the bioelectrical activity of the brain with different levels of serotonin in the serum of peripheral blood in young people 15-17 years old. The study involved 93 healthy girls and boys (15-17 years) of the Arkhangelsk region and the Nenets autonomous okrug. A serotonin level is determined in serum by enzyme immunoassay using a set of “Serotonin ELISA”. The electroencephalogram (EEG) power spectrum (PS) in the alpha, beta and theta frequencies ranges was recorded using an electroencephalograph “Encephalan” (Medicom, Taganrog). Age-dependent electroencephalogram (EEG) patterns is associated with the level of serotonin in peripheral blood in adolescents. On the background of a higher level of serotonin in the blood, compared to girls, boys have localized associations of theta and beta1 activity of EEG and serotonin levels, mainly in the right frontal-temporal region. In girls, the spectral power level of the EEG theta activity is more dependent on the level of serotonin in the blood, and a greater number of brain areas are involved in correlation interactions in comparison with young men (temporal regions on the left and frontal, central, parietal regions of both hemispheres of the brain).

An optimal electron-beam welding operating regime for ultra-pure sheet niobium has been developed for use in a superconducting resonator for the International Linear Collider (ILC). The formation of weld joints is studied and their microstructure and microhardness are investigated taking the required geometry of the weld seams into account. Low-temperature electrical measurements in magnetic fields up to 2 T are used to determine the critical parameters of the superconducting transition in the weld area. From the standpoint of the superconducting properties of the resonator, the slight degradation in the characteristics of sheet niobium observed in the thermally affected area (about 10% on average) is not of fundamental importance.

Ultracentrifugation on a density gradient remains the only reliable way to obtain highly pure mitochondria preparations. However, it is not readily available for any laboratory and has a serious disadvantage of providing low mitochondria yield, which can be critical when working with limited starting material. Here we describe a combined method for isolation of mitochondria for proteomic studies that includes cell disruption by sonication, differential centrifugation, and magnetic separation. Our method provides remarkable enrichment of mitochondrial proteins as compared to differential centrifugation, magnetic separation, or their combination, and it enables the strongest depletion of cytoplasmic components, as assessed by two-dimensional electrophoresis, mass spectrometry, and Western blot. It also doubles the yield of mitochondria. However, our method should not be used for functional studies as most of the isolated organelles demonstrate disturbed structure in electron microphotographs.

The mechanism for the muon catalyzed fusion reaction t + t → 4He + 2n + 11.33 MeV is investigated. The model of the cascade reaction with 5He as an intermediate state is considered, both the ground and the first exited states being taken into account. The neutron energy spectrum measured in the recent experiment is compared with the Monte-Carlo-simulated one. Varying reaction parameters, we obtain optimum values for the relative weights of the 5He ground and excited states and for the excitation energy and width of the excited state.

Pituitary tumor-transforming gene-1 (PTTG1) encodes securin, a multifunctional protein involved in development of various types of cancer. Securin participates in the regulation of sister chromatids separation and the expression of multiple genes involved in the control of the cell cycle, metabolism, and angiogenesis. In several human cell lines, we have found a novel short isoform of securin mRNA, which does not contain exons 3 and 4. After the translation of this new mRNA, a shortened protein is produced that, like the full-size form, is able to activate the transcription of cyclin D3 gene (CCND3), which controls the G1/S transition and angiogenesis factors VEGFA (vascular endothelial growth factor), and FGF2 (fibroblast growth factor 2) in HEK293 cells. However, unlike the full-size protein, the short isoform of PTTG1 does not affect the MYC gene expression because it lacks the DNA-binding domain, which is needed for its interactions with the MYC promoter. Furthermore, the short form of securin does not influence the expression of MYC transcriptional targets, such as TP53 and IL-8. Thus, we found a novel isoform of securin which is able to activate a more restricted repertoire of genes compared to the full-size protein.

We consider the rigorous problem of excitation of a metamaterial sphere by a meridionally directed electric dipole. High Q-resonances in the low-frequency region are found and studied. The existence conditions of these resonances are established. Frequency-amplitude characteristics of the sphere and the vector scattering patterns at resonant frequencies are calculated. Approximate analytical expressions for resonant frequencies are obtained. The effect of nonresonance field enhancement, which consists in that the scattered field exceeds the primary field in a wide frequency range, is found.

The review contains a definition and the essence of biofeedback functional methods. The historical, scientific aspects and the main hypotheses are given here explaining the mechanisms of biofeedback. Different techniques and methods of biofeedback are discussed, as well as the scope of the rapidly developing technology of biofeedback. Emphasis is put on physiological mechanisms and prospects of heart rate variability biofeedback methods of development.

A precision method for measuring of ultrahigh unloaded Q factors of superconducting cavities is proposed which is based on the excitation of oscillation in the cavity by electron beam. In this measuring, the cavity is not connected to any external microwave circuit; its unloaded Q factor is determined from the loss of electron-beam power, which can be measured with high accuracy.

The polyacrylamide gel electrophoresis of chernozem humic acids (HAs) followed by ultraviolet detection (λ = 312 nm) has revealed a new highly fluorescent fraction that has the highest electrophoretic mobility and the lowest nominal molecular weight (NMW). The preparative isolation of the fraction has been performed using the multiple microfiltration of the same HA sample in a 7 M carbamide solution on a membrane with a nominal pore size of 5 kDa. Thirty ultrafiltrates with NMW 5 kDa. Fluorescence maximums at and below 490 nm have been noted only in the first four ultrafiltrates. All the ultrafiltrates have been combined into the fraction with NMW < 5 kDa, which has been successively passed through membranes of 3 and 1 kDa. Solutions of subfractions F 3–5 kDa, F 1–3 kDa, and F < 1 kDa with fluorescence maximums at 505, 488, and 465 nm, respectively, have been prepared. The F < 1 kDa subfraction with the lowest NMW had the highest fluorescence intensity. The distribution of the fluorescence maximums in the ultrafiltrates has indicated the presence of at least two groups of fluorophores and has confirmed the supramolecular organization of the extracted soil HAs.

Приводятся основные результаты работ по созданию экспериментальной базы, позволяющей безопасно проводить фундаментальные и прикладные исследования с тритийсодержащими средами в условиях неспециализированных лабораторий. Обсуждаются технические характеристики комплексов оборудования и установок, методические подходы, обеспечившие проведение исследований: явления мюонного катализа ядерных реакций синтеза; свойств лёгких экзотических ядер на границе нейтронной стабильности; явления «сверхпроницаемости» трития через металлы V группы и проницаемости водорода через конструкционные материалы, в том числе содержащие радиогенный Не; взаимодействия конструкционных материалов с изотопами водорода, включая тритий при давлении до 300 МПа.

IL2RA gene encodes the alpha subunit of a high-affinity receptor for interleukin-2 which is expressed by several distinct populations of lymphocytes involved in autoimmune processes. A large number of polymorphic alleles of the IL2RA locus are associated with the development of various autoimmune diseases. With bioinformatics analysis we the dissected the first intron of the IL2RA gene and selected several single nucleotide polymorphisms (SNPs) that may influence the regulation of the IL2RA gene in cell types relevant to autoimmune pathology. We described five enhancers containing the selected SNPs that stimulated activity of the IL2RA promoter in a cell-type specific manner, and tested the effect of specific SNP alleles on activity of the respective enhancers (E1 to E5, labeled according to the distance to the promoter). The E4 enhancer with minor T variant of rs61839660 SNP demonstrated reduced activity due to disrupted binding of MEF2A/C transcription factors (TFs). Neither rs706778 nor rs706779 SNPs, both associated with a number of autoimmune diseases, had any effect on the activity of the enhancer E2. However, rare variants of several SNPs (rs139767239, rs115133228, rs12722502, rs12722635) genetically linked to either rs706778 and/or rs706779 significantly influenced the activity of E1, E3 and E5 enhancers, presumably by disrupting EBF1, GABPA and ELF1 binding sites.

The article concerns the technique of effective and accurate measurement high quality-factor of resonant cavities of Tesla-type that are used in the ILC in superconducting regime. Presented key outcomes that have been obtained using specially developed computerized measuring system with a set of extra UHF parts.

By means of muon catalysis we study the phenomena in a pt-fusion, which have been previously investigated in the only experiment and now are at the frontier of nuclear few-body physics. The experiment is aimed at measuring the yields of the reaction products: γ-quanta, conversion muons and e + e − pairs. As a result we plan to measure the pt-fusion partial product yields (first time for e + e − pairs) with accuracy not worse than 10%, and this will enable us to obtain the nuclear reaction rates in M1 and E0 transitions in A = 4 system.

Computations of the accelerator section of the International Linear Collider (ILC), which consists of superconducting niobium cavities, are performed for conditions of the maximum energy transfer to electrons that travel along the cavity axis. A mathematical model and software packages are created for the computation of the electric characteristics and profile of a single-cell cavity. A computer-based synthesis of the cavity shape that yields the required electric characteristics is performed. The promising design variants of a single-cell cavity, with which a quality factor of 1010 is provided at a working frequency of 1.3 GHz, are found to optimize the construction and manufacture of a single-cell cavity. The electric characteristics of a chain of single-cell cavities are computed.

An electro-optical trap for spatially indirect dipolar excitons has been implemented in a GaAs/AlAs Schottky diode with a 400-A-wide single quantum well. In the presence of a bias voltage applied to a gate, the trap for excitons appears upon ring illumination of the structure by a continuous-wave or pulsed laser generating hot electron-hole pairs in the quantum well. A barrier for excitons collected inside the illuminated ring appears because of the screening of the applied electric field by nonequilibrium carriers directly in the excitation region. Excitons are collected inside the ring owing to the ambipolar drift of carriers and dipole-dipole exciton repulsion in the optical pump region. For dipolar excitons thus collected in the center of the ring electrooptical trap, a significant narrowing of the luminescence line that accompanies an increase in the density of excitations indicates the collective behavior of dipolar excitons.

A search for the muon-catalyzed fusion reaction d + d → 4He + γ in the ddμ muonic molecule was performed using the experimental installation TRITON with BGO detectors for γ-quanta. A high-pressure target filled with deuterium was exposed to the negative muon beam of the JINR Phasotron to detect γ-quanta with the energy 23.8 MeV. An experimental estimation for the yield of radiative deuteron capture from the ddμ state J = 1 was obtained at the level of ηγ ≤ 8 × 10−7 per fusion.

A methodical system has been developed to solve the problem of isolating informative signals against the background of noise which is based on a morphological analysis. It is shown that the morphological method of analyzing infrasonic signals is an effective instrument to isolate infrasonic signals, to analyze the features of their forms, and to estimate their relative delays. The advantage that the morphological method has over the correlation method in analyzing short signal fragments recorded by infrasonic microphones spaced at distances that significantly exceed the signal wavelengths is shown.

The phase diagram of Bose-Einstein condensates of dipolar excitons accumulated in lateral electrostatic traps is determined. Spatially periodic luminescence structures of the exciton Bose condensate and their critical dependence on pump level and temperature are observed. Coherence in the dipolar exciton Bose-Einstein condensates is studied by direct measurement of the 1-st and 2-nd order correlators with variations in pump level and temperature. These results are indicative of large-scale coherence of the exciton Bose condensate.