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51. Tip-1 induces filopodia growth and is important for gastrulation movements during zebrafish development

Author

Jaya, Besser, Jelani T D, Leito, David L M, van der Meer, and Christoph P, Bagowski

Subjects
Homeodomain Proteins, Embryo, Nonmammalian, Recombinant Fusion Proteins, Green Fluorescent Proteins, Molecular Sequence Data, Animals, Amino Acid Sequence, Gastrula, Pseudopodia, RNA, Messenger, Cloning, Molecular, Zebrafish Proteins, Zebrafish
Abstract

Wnt signaling is essential during animal development and also plays important roles in pathological conditions. Two mayor pathways have been described: the beta-catenin-dependent canonical (or classical) pathway and the beta-catenin-independent non-canonical Wnt pathway. Recent binding studies suggest links between the small PDZ protein TIP-1 (Tax-1 interacting protein) to components of both Wnt pathways. We have cloned and characterized the zebrafish tip-1 gene. Whole mount in situ hybridization and semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) indicated that zebrafish tip-1 is present as a maternal RNA and is ubiquitously expressed during early development. After 24 h of development, tip-1 expression was high in the central nervous system (CNS) whereas only weak expression was detected in the caudal regions of the zebrafish embryo. Tip-1 knockdown using antisense morpholino oligonucleotides, as well as ectopic tip-1 expression, led to elongation defects in zebrafish embryos and larvae. Both knockdown and overexpression of tip-1 resulted in a widened goosecoid (gsc) expression domain in shield stage embryos, led to an abbreviated prechordal plate, and to reduced convergent extension movements during gastrulation. We constructed a green fluorescence protein (GFP)/TIP-1 fusion protein which, when expressed in cultured fibroblasts (ZF4-cells), induced filopodia growth. Our observations indicate a role for TIP-1 in gastrulation movements and in filopodia growth induction.

Published
2007

58. Qualidade da água em tempo de chuva no sistema de drenagem urbana da Ribeira da Póvoa

Author

David, L. M.

Abstract

Neste artigo apresentam-se e discutem-se resultados de qualidade da água obtidos, em tempo seco e chuvoso, em seis secções nos colectores e três secções nas ribeiras da bacia de drenagem da Ribeira da Póvoa. Em tempo chuvoso ocorreram descargas frequentes para as ribeiras com concentrações de cargas poluentes em muitos casos superiores às das águas residuais domésticas, devido ao arrastamento de substâncias previamente depositadas nas superfícies e nos colectores. Nos sistemas separativos, a afluência de águas residuais domésticas aos colectores pluviais, e a consequente formação de depósitos em tempo seco, poderá ter contribuído consideravelmente para o acréscimo das cargas poluentes descarregadas em tempo chuvoso. Devido a ligações indevidas de colectores pluviais aos colectores domésticos verificaram-se situações frequentes de entrada em carga nos colectores domésticos, bem como de descargas para as ribeiras através de descarregadores de tempestade instalados para o efeito. Nos sistemas unitários, concluiu-se que a existência de alguns trechos de colectores, ou de singularidades nos sistemas, com condições favoráveis à formação de depósitos terão sido responsáveis por aumentos significativos das cargas poluentes transportadas em tempo chuvoso. Assim, o funcionamento adequado dos sistemas separativos e a garantia de condições de auto-limpeza nos colectores unitários são factores de grande importância na redução das cargas poluentes descarregadas. Nas ribeiras, os aumentos de caudais em tempo chuvoso estiveram associados a acréscimos muito significativos das cargas poluentes, com concentrações da mesma ordem de grandeza e superiores até ás das águas residuais descarregadas pelos sistemas de colectores. Estes resultados podem ser explicados não só pela descarga directa de águas residuais dos sistemas de drenagem urbanos (efeito imediato das descargas), mas também pela ressuspensão de substâncias previamente depositadas no leito das ribeiras (efeito acumulado das descargas). ****** ****** ****** 16-19, Setembro, 2002 ******

Published
2002

59. Water wuality in Portuguese pseudo-separate and combined systems: a conceptual modelling approach for data comparison

Author

David, L. M.

Abstract

Six sewer sections of different combined and pseudo-separate catchments were continuously monitored for flow and sampled at preset intervals, over dry and wet weather periods. First flushes with high TSS and COD levels were observed in the outlet sewers of a 10 ha combined catchment and of an 8 ha stormwater pseudo-separate system. Higher TSS and COD concentrations were also found during the rain events in a 22 ha combined catchment. However, in two upstream 5 ha combined catchments, only a slight increase in TSS levels and no changes in COD were observed during the rain events. These results support that most of the flushed matter is stored in the larger and flatter combined sewers. Even for small intensity rain events, important increases in TSS, VTSS and COD were observed at an interceptor sewer serving several small pseudo-separate systems (80 ha). Values of all parameters tended to decrease over successive rain events and, in a third sub event, were even smaller than the dry-weather ones. By using a simple conceptual-modelling approach, a suitable and useful relationship between precipitation and TSS loads could be established for each of the six monitored sections. However additional experimental data are needed to fully calibrate the models. If calibrated for a wide range of rain events, possibly these would allow for the evaluation of the TSS loads for longer periods. ****** 10 pág. ****** 8-13, Setembro, 2002 ******

Published
2002

61. Avaliação e comparação entre métodos de mensuração de pressão arterial sistólica em gatos hígidos conscientes.

Author

Anjos, T. M., Veado, J. C. C., Araújo, R. B., Leme, F. O. P., Diniz, S. A., Castro, M. C. N., Costa, F. V. A., Tavares, C. A. P., David, L. M., Quites, K. X., and Maia, M. Q.

Abstract

Copyright of Arquivo Brasileiro de Medicina Veterinaria e Zootecnia is the property of Universidade Federal de Minas Gerais, Escola de Veterinaria and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2016
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63. Centre for application of mathematical modelling in the field of operational water management CAMM/CROWN

Author

Matos, R. S., David, L. M., Clare, A. F., Cardoso, M. A., and Almeida, M. C.

Abstract

Este registo pertence ao Repositório Científico do LNEC Made available in DSpace on 2010-04-26T09:19:12Z (GMT). No. of bitstreams: 3 stub.txt.txt: 74 bytes, checksum: 2201e90db4d96cbf54168f6263e126b0 (MD5) license.txt: 37 bytes, checksum: 562acc9db45b6d1b48b190c6b4653ecb (MD5) stub.txt: 73 bytes, checksum: 3bc2cc4245f8fae5b15427ea15f98b20 (MD5) Previous issue date: 1994-07 Made available in DSpace on 2014-10-20T13:25:53Z (GMT). No. of bitstreams: 3 stub.txt: 73 bytes, checksum: 3bc2cc4245f8fae5b15427ea15f98b20 (MD5) license.txt: 37 bytes, checksum: 562acc9db45b6d1b48b190c6b4653ecb (MD5) stub.txt.txt: 74 bytes, checksum: 2201e90db4d96cbf54168f6263e126b0 (MD5) Previous issue date: 1994-07

Published
1994

65. Centre for application of mathematical modelling in the field of operational water management CAMM/CROWN

Author

Matos, R. S., Almeida, M. C., Cardoso, M. A., and David, L. M.

Abstract

Este registo pertence ao Repositório Científico do LNEC Made available in DSpace on 2010-04-26T09:25:11Z (GMT). No. of bitstreams: 3 stub.txt.txt: 74 bytes, checksum: 2201e90db4d96cbf54168f6263e126b0 (MD5) license.txt: 37 bytes, checksum: 562acc9db45b6d1b48b190c6b4653ecb (MD5) stub.txt: 73 bytes, checksum: 3bc2cc4245f8fae5b15427ea15f98b20 (MD5) Previous issue date: 1995 Made available in DSpace on 2014-10-20T13:25:15Z (GMT). No. of bitstreams: 3 stub.txt: 73 bytes, checksum: 3bc2cc4245f8fae5b15427ea15f98b20 (MD5) license.txt: 37 bytes, checksum: 562acc9db45b6d1b48b190c6b4653ecb (MD5) stub.txt.txt: 74 bytes, checksum: 2201e90db4d96cbf54168f6263e126b0 (MD5) Previous issue date: 1995 NES

Published
1993

67. O ciclo urbano da água: metodologias e instrumentos de tratamento da informação relevante ao nível de um sistema municipal. Estudo da bacia piloto de Vila d'Este. Projecto NATO PO-RIVERS - Metodologias para o desenvolvimento dos recursos hídricos em Portugal, actividade N

Author

Almeida, M. C., Cardoso, M. A., David, L. M., Bicudo, J. R., Alegre, H., and Pinheiro, I.

Abstract

Este registo pertence ao Repositório Científico do LNEC Made available in DSpace on 2010-04-26T09:26:16Z (GMT). No. of bitstreams: 3 stub.txt.txt: 74 bytes, checksum: 2201e90db4d96cbf54168f6263e126b0 (MD5) license.txt: 37 bytes, checksum: 562acc9db45b6d1b48b190c6b4653ecb (MD5) stub.txt: 73 bytes, checksum: 3bc2cc4245f8fae5b15427ea15f98b20 (MD5) Previous issue date: 1992-11 Made available in DSpace on 2014-10-20T13:25:25Z (GMT). No. of bitstreams: 3 stub.txt: 73 bytes, checksum: 3bc2cc4245f8fae5b15427ea15f98b20 (MD5) license.txt: 37 bytes, checksum: 562acc9db45b6d1b48b190c6b4653ecb (MD5) stub.txt.txt: 74 bytes, checksum: 2201e90db4d96cbf54168f6263e126b0 (MD5) Previous issue date: 1992-11

Published
1992

68. Modelação matemática da rede de drenagem do Terreiro do Paço - Relatório final

Author

David, L. M.

Abstract

Este registo pertence ao Repositório Científico do LNEC Made available in DSpace on 2008-12-19T12:36:17Z (GMT). No. of bitstreams: 2 stub.txt: 73 bytes, checksum: 3bc2cc4245f8fae5b15427ea15f98b20 (MD5) license.txt: 37 bytes, checksum: 562acc9db45b6d1b48b190c6b4653ecb (MD5) Previous issue date: 1992-02 Made available in DSpace on 2010-04-26T09:20:27Z (GMT). No. of bitstreams: 3 stub.txt.txt: 74 bytes, checksum: 2201e90db4d96cbf54168f6263e126b0 (MD5) license.txt: 37 bytes, checksum: 562acc9db45b6d1b48b190c6b4653ecb (MD5) stub.txt: 73 bytes, checksum: 3bc2cc4245f8fae5b15427ea15f98b20 (MD5) Previous issue date: 1992-02 Made available in DSpace on 2014-10-20T13:26:04Z (GMT). No. of bitstreams: 3 stub.txt: 73 bytes, checksum: 3bc2cc4245f8fae5b15427ea15f98b20 (MD5) license.txt: 37 bytes, checksum: 562acc9db45b6d1b48b190c6b4653ecb (MD5) stub.txt.txt: 74 bytes, checksum: 2201e90db4d96cbf54168f6263e126b0 (MD5) Previous issue date: 1992-02 NHS

Published
1992

69. Modelação matemática da rede de drenagem do Terreiro do Paço - Relatório preliminar

Author

David, L. M.

Abstract

Este registo pertence ao Repositório Científico do LNEC Made available in DSpace on 2008-12-19T12:36:19Z (GMT). No. of bitstreams: 2 stub.txt: 73 bytes, checksum: 3bc2cc4245f8fae5b15427ea15f98b20 (MD5) license.txt: 37 bytes, checksum: 562acc9db45b6d1b48b190c6b4653ecb (MD5) Previous issue date: 1992-02 Made available in DSpace on 2010-04-26T09:23:00Z (GMT). No. of bitstreams: 3 stub.txt.txt: 74 bytes, checksum: 2201e90db4d96cbf54168f6263e126b0 (MD5) license.txt: 37 bytes, checksum: 562acc9db45b6d1b48b190c6b4653ecb (MD5) stub.txt: 73 bytes, checksum: 3bc2cc4245f8fae5b15427ea15f98b20 (MD5) Previous issue date: 1992-02 Made available in DSpace on 2014-10-20T13:24:54Z (GMT). No. of bitstreams: 3 stub.txt: 73 bytes, checksum: 3bc2cc4245f8fae5b15427ea15f98b20 (MD5) license.txt: 37 bytes, checksum: 562acc9db45b6d1b48b190c6b4653ecb (MD5) stub.txt.txt: 74 bytes, checksum: 2201e90db4d96cbf54168f6263e126b0 (MD5) Previous issue date: 1992-02 1152 - arquivado na SE do DHA. Proc 066/1/10219. Rel 87/92-NHS NHS

Published
1992

70. O ciclo urbano da água: metodologias e instrumentos de tratamento da informação relevante ao nível concelhio. O caso de Vila nova de Gaia. Projecto NATO PO-RIVERS - Metodologias para o desenvolvimento dos recursos hídricos em Portugal, actividade N

Author

Cardoso, M. A., David, L. M., Bicudo, J. R., Matos, R. S., Almeida, M. C., and Alegre, H.

Abstract

Este registo pertence ao Repositório Científico do LNEC Made available in DSpace on 2010-04-26T09:22:04Z (GMT). No. of bitstreams: 3 stub.txt.txt: 74 bytes, checksum: 2201e90db4d96cbf54168f6263e126b0 (MD5) license.txt: 37 bytes, checksum: 562acc9db45b6d1b48b190c6b4653ecb (MD5) stub.txt: 73 bytes, checksum: 3bc2cc4245f8fae5b15427ea15f98b20 (MD5) Previous issue date: 1992 Made available in DSpace on 2014-10-20T13:24:45Z (GMT). No. of bitstreams: 3 stub.txt: 73 bytes, checksum: 3bc2cc4245f8fae5b15427ea15f98b20 (MD5) license.txt: 37 bytes, checksum: 562acc9db45b6d1b48b190c6b4653ecb (MD5) stub.txt.txt: 74 bytes, checksum: 2201e90db4d96cbf54168f6263e126b0 (MD5) Previous issue date: 1992

Published
1992

71. Canal de ensaios para artes de pesca. 4.º relatório

Author

Martins, R. B., David, L. M., Castro, M. P., and Palma, J.

Abstract

Este registo pertence ao Repositório Científico do LNEC Made available in DSpace on 2008-12-19T11:41:02Z (GMT). No. of bitstreams: 2 stub.txt: 73 bytes, checksum: 3bc2cc4245f8fae5b15427ea15f98b20 (MD5) license.txt: 37 bytes, checksum: 562acc9db45b6d1b48b190c6b4653ecb (MD5) Previous issue date: 1991-07 Made available in DSpace on 2010-04-26T13:19:48Z (GMT). No. of bitstreams: 3 stub.txt.txt: 74 bytes, checksum: 2201e90db4d96cbf54168f6263e126b0 (MD5) license.txt: 37 bytes, checksum: 562acc9db45b6d1b48b190c6b4653ecb (MD5) stub.txt: 73 bytes, checksum: 3bc2cc4245f8fae5b15427ea15f98b20 (MD5) Previous issue date: 1991-07 Made available in DSpace on 2014-10-20T09:29:54Z (GMT). No. of bitstreams: 3 stub.txt: 73 bytes, checksum: 3bc2cc4245f8fae5b15427ea15f98b20 (MD5) license.txt: 37 bytes, checksum: 562acc9db45b6d1b48b190c6b4653ecb (MD5) stub.txt.txt: 74 bytes, checksum: 2201e90db4d96cbf54168f6263e126b0 (MD5) Previous issue date: 1991-07 1114 - arquivado na SE do DHA. Proc 060/1/9597. Rel 155/91-DH/CPCE NRE

Published
1991

78. Combined sewer overflow emissions to bathing waters in Portugal. How to reduce in densely urbanised areas?

Author

David, L. M. and Matos, J. S.

Subjects
*WATER quality management, *COMBINED sewer overflows, *WATER quality, *URBAN watersheds, *URBAN runoff management, *MUNICIPAL water supply
Abstract

The current proposal for a revised European Directive concerning the quality of bathing water significantly increases the demands for the control of wet-weather discharges. A densely urbanised combined catchment was modelled for a 19-year long rainfall series in order to assess the current situation and to evaluate the benefits of potential solutions. Storage and advanced physical-chemical treatment of stormwater in the STP may significantly contribute for the reduction of the overflow volumes but reductions of the spill frequency under 2.5 spill days per bathing season are hardly obtained. This study reveals the severe strains that the local rainfall pattern may place on the control of the frequency of wet-weather discharges, pointing to serious technical, social and economical implications, at the local and at the national level, if the current proposal for a revised European Directive on Bathing Water is enforced. [ABSTRACT FROM AUTHOR]

Published
2005
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79. Wet-weather urban discharges: implications from adopting the revised European Directive concerning the quality of bathing water.

Author

David, L. M. and Matos, J. S.

Subjects
*WATER pollution laws, *FECAL contamination, *BEACHES, *URBAN runoff management, *COMBINED sewers, *STORMWATER infiltration, *DEPTH-area-duration (Hydrometeorology)
Abstract

Wet weather urban discharges are responsible for bathing water contamination. The proposal for a revised EU Directive concerning the quality of bathing water imposes significantly more stringent requirements for the management of bathing water quality, with particularly important repercussions on beaches subjected to short-term pollution incidents. The paper reviews the aspects from EU legislation most directly related to the problem of wet-weather discharges, placing special emphasis on the recent revision process of the Directive on bathing water quality, and evaluates the benefits of some potential solutions based on continuous modelling of a combined sewer system. Increasing the sewer system storage capacity or the STP hydraulic capacity may substantially reduce the untreated discharge volumes, but spill frequency reductions under 2 to 3 spill days per bathing season will hardly be achieved. Results show the severe strains that local rainfall patterns would place on compliance with the Commission's proposal for a revised Directive and highlight the importance of the changes introduced in the amended proposal recently approved by the Council, making it less prescriptive if adequate measures are adopted to prevent bathers' exposure to short-term pollution incidents. [ABSTRACT FROM AUTHOR]

Published
2005
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80. Misuse Made Plain: Evaluating Concerns About Neuroscience in National Security.

Author

Lowenberg, Kelly, Simon, Brenda M., Burns, Amy, Greismann, Libby, Halbleib, Jennifer M., Persad, Govind, Preston, David L. M., Rhodes, Harker, and Murphy, Emily R.

Subjects
NEUROSCIENCE equipment, NATIONAL security, MAGNETIC resonance imaging, LIE detectors & detection, ETHICS
Abstract

In this article the authors discuss the misuse of neuroscience in national security. The authors stress on the validity and reliability of neuroscientific applications, particularly the utilization of functional magnetic resonance imaging (fMRI)-based lie detection. Furthermore, the authors are critical on Jonathan Marks' neuroskepticism towards the legitimacy of neuroscientific technologies and its capability to enhance interrogation techniques.

Published
2010
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81. Modeling fecal contamination in the Aljezur coastal stream (Portugal)

Author

Rodrigues, M., Anabela Oliveira, Guerreiro, M., Fortunato, A. B., Menaia, J., David, L. M., and Cravo, A.

Abstract

This study aims at understanding the fecal contamination behavior in a small coastal stream (Aljezur, Portugal), which has significant economic and ecological values. Like in most small coastal systems, circulation and water renewal in the Aljezur stream exhibit a strong variability due to their dependence on tides, waves, intermittent river flows, and a highly variable morphology. Hence, the problem was approached through a combination of field surveys and the development and application of a hard-coupled three-dimensional hydrodynamic and fecal contamination model. Salinity and temperature results have shown that mixing and transport in the stream are very sensitive to the river flow and wind forcing. The model is able to represent the main patterns and trends observed in Escherichia coli and fecal enterococcus concentrations along the stream, for different environmental and contamination conditions, suggesting die-off rates on the order of 0.50–0.55 day−1. Die-off rate and the representation of the sediment-associated processes were identified as the major remaining sources of uncertainty in the model. Results show that, owing to the processes that occur along the stream, fecal bacteria reach the beaches water in numbers that comply with the European Bathing Waters Directive, even during the summer periods when the upstream concentrations are larger. In particular, results suggest a direct relation between the tidal propagation upstream and the reduction of the fecal bacteria concentrations along the stream that can be relevant for the development of a strategy for the management of the system’s water safety. 841–856 pp 61 DHA/NEC Ocean Dynamics

92. Peritoneal metastases of lower gastrointestinal tract origin:a comparative study of patient outcomes following cytoreduction and intraperitoneal chemotherapy.

Author

Ung L, Chua TC, and David L M

Subjects
Adenocarcinoma drug therapy, Adenocarcinoma pathology, Adenocarcinoma surgery, Aged, Appendiceal Neoplasms drug therapy, Appendiceal Neoplasms pathology, Appendiceal Neoplasms surgery, Colonic Neoplasms drug therapy, Colonic Neoplasms pathology, Colonic Neoplasms surgery, Combined Modality Therapy, Disease-Free Survival, Female, Humans, Intestinal Neoplasms drug therapy, Intestinal Neoplasms surgery, Male, Middle Aged, Peritoneal Neoplasms drug therapy, Peritoneal Neoplasms surgery, Rectal Neoplasms drug therapy, Rectal Neoplasms pathology, Rectal Neoplasms surgery, Retrospective Studies, Survival Rate, Intestinal Neoplasms pathology, Intestinal Neoplasms therapy, Peritoneal Neoplasms secondary, Peritoneal Neoplasms therapy
Abstract

Background: Cytoreductive surgery (CS) combined with intraperitoneal chemotherapy (IPC) is a multimodal approach to the treatment of peritoneal metastases (PM) of lower gastrointestinal origin. This study examines patient outcomes and critically evaluates its patterns of recurrences relative to the site of metastatic origin., Methods: Patients treated with CS/IPC from 2000 to 2012 where PM arose from a primary tumour of the appendix, colon and rectum were identified from a prospective database for retrospective evaluation. The primary endpoints were survival (overall and disease-free), and secondary end points include patterns of recurrence and prognostic factors associated with overall outcomes., Results: Two hundred and eleven patients were followed up for a median of 23.3 months (range 1–156). Overall median survival was 46.8 months, and the 1-, 3-, 5-year survival rates were 87, 56 and 42 %, respectively. The 5-year survival of patients with appendiceal, colonic and rectal PM was 55, 33 and 20 %, respectively. Tumour origin was the only independent prognostic factor associated with overall survival (p = 0.03). Recurrences were more common in patients of colorectal origin over appendiceal origin (p\0.001) and were more likely to be of a systemic nature (p = 0.05)., Conclusion: CS/IPC provides an option for improved survival in patients with PM of lower gastrointestinal origin and appears to be most promising in patients with disease of appendiceal origin.

Published
2013
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93. Key issues for sustainable urban stormwater management.

Author

Barbosa AE, Fernandes JN, and David LM

Subjects
Community Participation, Decision Making, Environment, Environmental Monitoring, Public Health, Water Pollutants, Water Pollution prevention & control, Cities, Conservation of Natural Resources methods, Drainage, Sanitary methods, Waste Disposal, Fluid methods
Abstract

Since ancient times, it is understood that stormwater from constructed areas should be managed somehow. Waste and pollution transported by stormwater poses quantity and quality problems, affecting public health and the quality of the environment. Sanitation infrastructures in urbanized regions have different development levels and the perception of stormwater changed considerably during the centuries and especially in recent years. Still, there is an evident worldwide heterogeneity when analyzing the lack of studies on urban stormwater conducted in some Asian or African countries. Strategies for sustainable stormwater management are needed at different decision levels (political, regional or local scale, for instance) but all of them need information and a clear understanding of the possibilities that are at stake as well as the main consequences of each decision. A sound approach to stormwater management should be flexible, based on local characteristics, and should take into consideration temporal, spatial and administrative factors and law, among other issues. Economic or technical constraints define different decision scenarios. Best Management Practices should be seen as an opportunity for development and improvement of social, educational and environmental conditions in urbanized and surrounding areas. Therefore they require an ample perspective and the participation of different stakeholders. High-quality decision needs time and a fair overview of the problem: the purpose of this document is to contribute to sustainable stormwater management, informing on the most relevant factors that should be assessed and their interaction. A flowchart has been produced and is presented, indicating the most relevant steps, processes and information that should be taken into account in urban development., (Copyright © 2012 Elsevier Ltd. All rights reserved.)

Published
2012
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94. Zebrafish cypher is important for somite formation and heart development.

Author

van der Meer DL, Marques IJ, Leito JT, Besser J, Bakkers J, Schoonheere E, and Bagowski CP

Subjects
Alternative Splicing, Amino Acid Sequence, Animals, Animals, Genetically Modified, Heart physiology, Homeodomain Proteins genetics, LIM Domain Proteins, Molecular Sequence Data, Muscle, Skeletal embryology, Muscle, Skeletal metabolism, Myocardium metabolism, Organ Specificity, Protein Structure, Tertiary, RNA, Messenger, Stored metabolism, Zebrafish physiology, Zebrafish Proteins genetics, Heart embryology, Homeodomain Proteins metabolism, Somites physiology, Zebrafish embryology, Zebrafish Proteins metabolism
Abstract

Mammalian CYPHER (Oracle, KIA0613), a member of the PDZ-LIM family of proteins (Enigma/LMP-1, ENH, ZASP/Cypher, RIL, ALP, and CLP-36), has been associated with cardiac and muscular myopathies. Targeted deletion of Cypher in mice is neonatal lethal possibly caused by myopathies. To further investigate the role of cypher in development, we have cloned the zebrafish orthologue. We present here the gene, domain structure, and expression pattern of zebrafish cypher during development. Cypher was not present as a maternal mRNA and was absent during early development. Cypher mRNA was first detected at the 3-somite stage in adaxial somites, and as somites matured, cypher expression gradually enveloped the whole somite. Later, cypher expression was also found in the heart, in head and jaw musculature, and in the brain. We further identified 13 alternative spliced forms of cypher from zebrafish heart and skeletal muscle tissue, among them a very short form containing the PDZ domain but lacking the ZM (ZASP-like) motif and the LIM domains. Targeted gene knock-down experiments using cypher antisense morpholinos led to severe defects, including truncation of the embryo, deformation of somites, dilatation of the pericardium, and thinning of the ventricular wall. The phenotype could be rescued by a cypher form, which contains the PDZ domain and the ZM motif, but lacks all three LIM domains. These findings indicate that a PDZ domain protein is important for normal somite formation and in normal heart development. Treatment of zebrafish embryos with cyclopamine, which disrupts hedgehog signaling, abolished cypher expression in 9 somite and 15-somite stage embryos. Taken together, our data suggest that cypher may play a role downstream of sonic hedgehog, in a late stage of somite development, when slow muscle fibers differentiate and migrate from the adaxial cells.

Published
2006
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95. Wet weather water quality modelling of a Portuguese urban catchment: difficulties and benefits.

Author

David LM and Matos RS

Subjects
Cities, Geologic Sediments, Portugal, Sewage, Water Movements, Models, Theoretical, Water Pollutants analysis, Water Supply, Weather
Abstract

This paper discusses the use of water quality deterministic modelling together with an integrated approach to assess the impact of urban stormwater discharges into ephemeral watercourses, based on the study of a Portuguese catchment. The description of the main aspects, difficulties and benefits found during data collection and model calibration and verification is presented, and the associated uncertainties and errors discussed. Experimental results showed a strong short- and long-term impact of sewer discharges on rivers, and confirmed deposition, resuspension and transport of pollutants as important processes for the water quality. However, the resuspension of riverbed sediment pollutants during storms was probably more significant than the direct impact of the urban discharges. The HydroWorks model was used since it allows for the calculation of pollutant build-up on catchment surfaces and in gully pots, their wash-off, and the deposition and erosion of sediments in sewers. However, it uses several constants, which could not be independently calibrated, increasing the uncertainty already associated with the data. River flows have quite different magnitude from the sewer system overflows, which, together with the difficulties in evaluating river flow rates, makes the integrated modelling approach rather complex and costly.

Published
2002

97. In my hands...

Author

David LM

Subjects
Humans, Outcome and Process Assessment, Health Care, Attitude of Health Personnel, Dermatology instrumentation, Laser Therapy instrumentation
Published
1999

99. Laser resurfacing: a safe and predictable method of skin resurfacing.

Author

Lent WM and David LM

Subjects
Acne Vulgaris complications, Adult, Aged, Aged, 80 and over, Cicatrix etiology, Cicatrix surgery, Female, Humans, Male, Middle Aged, Skin Aging, Dermatologic Surgical Procedures, Face surgery, Laser Therapy adverse effects, Laser Therapy methods
Abstract

Skin resurfacing with lasers for skin restoration has been the most exciting advance in cosmetic surgery in the past 2 years. This method has effectively replaced chemical peel and dermabrasion as modes for skin rejuvenation, yet surprisingly, very little has been published on its effectiveness. Our aim is to delineate clear indications, methods and results of laser skin resurfacing. Laser resurfacing has evolved over the past 13 years and our current method has emerged from this experience. This method has been employed on 258 consecutive patients treated over the past 2 years. A total of 201 patients were treated for photodamaged skin, 53 for facial acne scarring and four for post-surgical scars. Each of these conditions has in common the pathological finding of contour irregularity of the involved abnormal skin. The CO2 Ultrapulse laser's advantage is the predictable ability to vaporize the upper layer of elevated skin, tighten collagen bundles and induce a local inflammatory reaction with epithelial proliferation. This paper presents a clear, concise, and conservatively oriented technique, including pre- and post-operative care of laser skin resurfacing. The technique presented is conceived to correct precisely the underlying pathological condition and can be used in conjunction with other cosmetic procedures. A total of 200 patients underwent full face resurfacing, 41 periorbital, 13 perioral and four for facial scars. Of the patients, 78% were female and 22% were male. The results of each patient were judged by an independent observer. Some 92% were found to have either an excellent or very good result; 2% were judged to be satisfactory; and 6% were from outside the region or were lost to follow-up. Minor complications included 12% transient post-inflammatory hyperpigmentation, 3% acute acne and 1% herpes type 1. There were no major complications, including hypertrophic scarring. A total of 15% required some type of secondary touch-up resurfacing. Our method of laser skin resurfacing offers a consistent method for dramatic improvement of facial skin quality for those patients with photodamage and acne scarring, while minimizing risk.

Published
1999
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