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72 results on '"Devanga Ragupathi, Naveen Kumar"'

53. Methicillin-Susceptible Teicoplanin-Resistant Staphylococcus haemolyticus Isolate from a Bloodstream Infection with Novel Mutations in the tcaRAB Teicoplanin Resistance Operon

Author

Bakthavatchalam, Yamuna Devi, primary, Sudarsanam, Thambu David, additional, Babu, Priyanka, additional, Munuswamy, Elakkiya, additional, Muthuirulandi Sethuvel, Dhiviya Prabaa, additional, Devanga Ragupathi, Naveen Kumar, additional, and Veeraraghavan, Balaji, additional

Published
2017
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56. First Report on the Draft Genome Sequences of Corynebacterium diphtheriae Isolates from India

Author

Veeraraghavan, Balaji, primary, Anandan, Shalini, additional, Rajamani Sekar, Suresh Kumar, additional, Gopi, Radha, additional, Devanga Ragupathi, Naveen Kumar, additional, Ramesh, Srilekha, additional, Verghese, Valsan Philip, additional, Korulla, Sophy, additional, Mathai, Sarah, additional, Sangal, Lucky, additional, and Joshi, Sudhir, additional

Published
2016
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63. Comparative studies to evaluate relative in vitro potency of luteolin in inducing cell cycle arrest and apoptosis in HaCaT and A375 cells

Author

Rangasamy Ashok Kumar, Vazhapilly Cijo George, Sanjay Kumar, Devanga Ragupathi Naveen Kumar, and P.K. Suresh

Subjects
Keratinocytes, Cancer Research, Programmed cell death, Skin Neoplasms, Free Radicals, Epidemiology, Cell Survival, Iron, Apoptosis, DNA Fragmentation, Biology, chemistry.chemical_compound, Picrates, Cell Line, Tumor, Humans, Fragmentation (cell biology), Luteolin, Melanoma, Cell Proliferation, Cell growth, Biphenyl Compounds, Public Health, Environmental and Occupational Health, Free Radical Scavengers, Cell cycle, Molecular biology, G1 Phase Cell Cycle Checkpoints, Cell biology, HaCaT, Oncology, chemistry, Cancer cell, M Phase Cell Cycle Checkpoints
Abstract

Luteolin is a naturally occurring flavonoid present in many plants with diverse applications in pharmacology. Despite several studies elucidating its significant anti-cancer activity against various cancer cells, the mechanism of action in skin cancer is not well addressed. Hence, we investigated the effects of luteolin in HaCaT (human immortalized keratinocytes) and A375 (human melanoma) cells. The radical scavenging abilities of luteolin were determined spectrophotometrically, prior to a cytotoxic study (XTT assay). Inhibitory effects were assessed by colony formation assay. Further, the capability of luteolin to induce cell cycle arrest and apoptosis were demonstrated by flow cytometry and cellular DNA fragmentation ELISA, respectively. The results revealed that luteolin possesses considerable cytotoxicity against both HaCaT and A375 cells with IC50 values of 37.1 μM and 115.1 μM, respectively. Luteolin also inhibited colony formation and induced apoptosis in a dose and time-dependent manner by disturbing cellular integrity as evident from morphological evaluation by Wright- Giemsa staining. Accumulation of cells in G2/M (0.83-8.14%) phase for HaCaT cells and G0/G1 (60.4-72.6%) phase for A375 cells after 24 h treatment indicated cell cycle arresting potential of this flavonoid. These data suggest that luteolin inhibits cell proliferation and promotes cell cycle arrest and apoptosis in skin cancer cells with possible involvement of programmed cell death, providing a substantial basis for it to be developed into a potent chemopreventive template for skin cancer.

Published
2013

65. First Indian report on B4/H24RxC ST410 multidrug-resistant Escherichia colifrom bloodstream infection harbouring blaOXA-181and blaCTX-M-15

Author

Devanga Ragupathi, Naveen Kumar, Vasudevan, Karthick, Venkatesan, Manigandan, and Veeraraghavan, Balaji

Abstract

Escherichia coliis regarded as one of the most commonly isolated Gram-negative pathogens from bloodstream infections. Increasing antimicrobial resistance (AMR) among E. coliis a threat to disease management as well as further dissemination of AMR genes to other clinically important pathogens. Here we report the genome of a multidrug-resistant (MDR) E. coli(BA22372) from a bloodstream infection belonging to ST410 B4/H24RxC subtype from India.

Published
2020
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66. First report of blaOXA-181-mediated carbapenem resistance in Aeromonas caviaein association with pKP3-A: Threat for rapid dissemination

Author

Anandan, Shalini, Gopi, Radha, Devanga Ragupathi, Naveen Kumar, Muthuirulandi Sethuvel, Dhiviya Prabaa, Gunasekaran, Priya, Walia, Kamini, and Veeraraghavan, Balaji

Abstract

•Mechanism of carbapenem resistance in Aeromonas caviaestudied by next-generation sequencing (NGS).•NGS revealed the resistance mechanism to be blaOXA-181.•blaOXA181was found in association with Tn2013in plasmid pKP3-A, which is a threat for rapid dissemination.•An IncQ2-like plasmid was found to harbour qnrS2resulting in fluoroquinolone resistance in A. caviae.•First observation of blaOXA-181in Aeromonasspp., adding to complexity for control of spread of carbapenem resistance.

Published
2017
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67. Draft genome sequence of reduced teicoplanin-susceptible and vancomycin-heteroresistant methicillin-resistant Staphylococcus aureusfrom sepsis cases

Author

Bakthavatchalam, Yamuna Devi, Veeraraghavan, Balaji, Devanga Ragupathi, Naveen Kumar, Babu, Priyanka, Munuswamy, Elakkiya, and David, Thambu

Abstract

Here we report the whole-genome shotgun sequence of six methicillin-resistant Staphylococcus aureus(MRSA) showing reduced susceptibility to both vancomycin and teicoplanin. The typical Indian community-acquired MRSA (CA-MRSA) clone ST772-MRSA-V-t657 was the most common genotype (3/6; 50%), followed by ST672-MRSA-IV (2/6; 33%) and ST22-MRSA-IV (1/6; 17%). All strains harboured a mutation in the tcaRABoperon, vraSR, graSRand/or rpoBgenes, which are frequently mutated determinants in a heteroresistant vancomycin-intermediate S. aureus(hVISA) phenotype.

Published
2017
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68. Pefloxacin as a Surrogate Marker for Fluoroquinolone Susceptibility for Salmonella typhi: Problems and Prospects.

Author

VEERARAGHAVAN, BALAJI, ANANDAN, SHALINI, MUTHUIRULANDI SETHUVEL, DHIVIYA PRABAA, and DEVANGA RAGUPATHI, NAVEEN KUMAR

Subjects
TREATMENT effectiveness, CIPROFLOXACIN, SALMONELLA typhi
Abstract

The article discusses the study on the effectiveness of pefloxacin disc diffusion with ciprofloxacin disc diffusion as prospect surrogate marker for the fluoroquinolone susceptibility of Salmonella typhi bacteria.

Published
2016
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70. Draft genome sequence of blaTEM-1-mediated cephalosporin-resistant Salmonella entericaserovar Typhi from bloodstream infection

Author

Devanga Ragupathi, Naveen Kumar, Muthuirulandi Sethuvel, Dhiviya Prabaa, Shankar, Baby Abirami, Munusamy, Elakkiya, Anandan, Shalini, and Veeraraghavan, Balaji

Abstract

Enteric fever is a major cause of concern in developing countries across the globe. The primary choice of antibiotics remains fluoroquinolones, followed by cephalosporins. Resistance to third-generation cephalosporins is rarely reported in Salmonella entericaserovar Typhi. This study reports the whole genome sequence of an S.Typhi isolate resistant to cefixime [minimum inhibitory concentration (MIC)=512μg/mL] by microbroth dilution. Interestingly, the isolate was negative for the cephalosporin resistance gene blaCTX-Mby PCR, which is a known mechanism for higher cephalosporin resistance. The isolate was further subjected to next-generation sequencing that identified blaTEM-1Band blaDHA-1genes in association with qnrB4and sul1. blaTEMis a known gene coding for β-lactam resistance. In certain cases, overexpression of blaTEMwas reported to result in cephalosporin resistance. This suggests that the high cefixime MIC would have been contributed by overexpression of blaTEM-1B. The blaTEM-1Bgene was found to be associated with a promoter Pxwith −35 and −10 regions as TTAATA and TAAAGT, respectively. The promoter regions were unique, but the −10 region was similar to that found in Pa/Pb(previously reported promoter for blaTEM) with a single nucleotide change. In addition, an IncN plasmid was identified, which is usually reported in association with the most prevalent extended-spectrum β-lactamase (ESBL), metallo- and non-metallo-carbapenemase, and plasmid-mediated quinolone resistance (PMQR) genes. Plasmids such as IncN might possibly confer resistance and enhance spread. It is imperative to continuously monitor the drug resistance profile and evolving genetic elements.

Published
2016
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72. Genetic Diversity of Clinical Bordetella Pertussis ST2 Strains in comparison with Vaccine Reference Strains of India.

Author

Sharma NC, Anandan S, Devanga Ragupathi NK, Muthuirulandi Sethuvel DP, Vasudevan K, Kumar D, Gupta SK, Sangal L, and Veeraraghavan B

Abstract

Objectives: Pertussis is a highly contagious disease of the respiratory tract caused by Bordetella pertussis , a bacterium that lives in the mouth, nose, and throat. Current study reports the highly accurate complete genomes of two clinical B. pertussis strains from India for the first time. Methods: Complete genome sequencing was performed for two B. pertussis strains using Ion Torrent PGM and Oxford nanopore sequencing method. Data was assembled de novo and the sequence annotation was performed through PATRIC and NCBI server. Downstream analyses of the isolates were performed using CGE server databases for antimicrobial resistance genes, plasmids, and sequence types. The phylogenetic analysis was performed using Roary. Results: The analysis revealed insertional elements flanked by IS 481 , which has been previously regarded as the important component for bacterial evolution. The two B. pertussis clinical strains exhibited diversity through genome degradation when compared to whole-cell vaccine reference strains of India. These isolates harboured multiple genetic virulence traits and toxin subunits, which belonged to sequence type ST2. Conclusion: The genome information of Indian clinical B. pertussis strains will serve as a baseline data to decipher more information on the genome evolution, virulence factors and their role in pathogenesis for effective vaccine strategies., Competing Interests: Competing Interests: The authors have declared that no competing interest exists., (© The author(s).)

Published
2021
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